Rabbit anti h3k9me3

J-Lat A2 cells were fixed with 1% formaldehyde for 10 min at room temperature, and ChIP assays were performed using the ChIP-IT Express Enzymatic kit (Active Motif 53009) according to the manufacturer’s protocol. For each ChIP reaction, 2 μg of the following antibodies were used: control rabbit IgG (Bethyl P120–101), rabbit anti-H3 (Invitrogen PA5–31954), rabbit anti-H4 (Invitrogen 720166), rabbit anti-H3K9me3 (Active Motif 39765), rabbit anti-H3K27me3 (Active Motif 39155), rabbit anti-Ac-H3 (Millipore 06–599), rabbit anti-Ac-H4 (Millipore 06–598), rabbit-anti-H4K5,8,12Ac (Invitrogen pa5–40083), mouse-anti-H4K16Ac (Invitrogen MA5–27794), rabbit anti-Brd4 (Thermo Fisher 702448), mouse anti-FLAG (Sigma F1804), rabbit anti-KAT8 (Active Motif 61245), rabbit anti-BRG1 (Cell Signaling 49360), and rabbit anti-ARID1A (Cell Signaling 12354). qPCR was performed with the primers listed in Table S1. Signals obtained by qPCR were normalized to those of input DNA, and the averages from triplicate qPCR reactions were used to generate standard deviation depicted by error bars. Two-tailed Student’s t-tests were conducted, and the different significance levels were marked by 1–3 asterisks (*: p < 0.05, **: p < 0.01, and ***: p < 0.001).