CCK-8 (Dojindo, Japan) assay was performed to evaluate the cell viability by measuring the absorbance at 450 nm (OD450) using BioTek Gen5 Microplate Reader (BioTek Instruments, USA). A colony formation assay was performed to evaluate the colony formation ability of the specific cancer types, following the method described in our previous study (25 (link)).
Biotek gen5 microplate reader
Manufactured by Agilent Technologies
Sourced in United States
The BioTek Gen5 Microplate Reader is a versatile instrument designed for performing a variety of microplate-based assays. It is capable of measuring absorbance, fluorescence, and luminescence in 6- to 384-well microplates. The reader provides accurate and reliable data acquisition to support a range of applications, including cell-based assays, protein quantification, and enzyme activity measurements.
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Lab products found in correlation
2 protocols using biotek gen5 microplate reader
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Cell Viability and Colony Formation Assays
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Cell Viability and Clonogenic Assays
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Cell viability was evaluated using CCK-8 assay (Dojindo, Japan). A total of 3×103 cells per well were seeded in 96-well plates and incubated for 24 h before the transfection. After 24, 48, 72, or 96 h of transfection, CCK8 was added to the culture, followed by 1 h incubation. The absorbance at 450 nm (OD450) was measured using the BioTek Gen5 Microplate Reader (BioTek Instruments, USA).
For the clonogenic assay, 6-well plates were seeded with 300 cells per well and incubated for two weeks till the colonies could be observed by naked eye. The cells were then fixed with 4% methanol and stained with 0.1% crystal violet. The number of colonies was captured by an Olympus camera (Tokyo, Japan) and counted by ImageJ.
For the clonogenic assay, 6-well plates were seeded with 300 cells per well and incubated for two weeks till the colonies could be observed by naked eye. The cells were then fixed with 4% methanol and stained with 0.1% crystal violet. The number of colonies was captured by an Olympus camera (Tokyo, Japan) and counted by ImageJ.
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