Veleta 2k 2k ccd camera

Testis and cauda epididymis samples were prepared as previously described (Shimada et al., 2019 (link)). The ultrathin sections were observed using a JEM-1400 plus electron microscope (JEOL) at 80 kV with a CCD Veleta 2K×2K camera (Olympus).

The sections of testes and cauda epididymis were prepared as previously described (Shimada et al., 2019 (link)). The prepared sections were observed using a JEM-1400 plus electron microscope (JEOL, Tokyo, Japan) at 80 ​kV with a CCD Veleta 2 ​K ​× ​2 ​K camera (Olympus).

The epididymis dissected from mice (at age of 9–12 weeks) was immersed overnight in cacodylate buffer (pH 7.4) containing 2.5% glutaraldehyde, washed with PBS, fixed with 1% OsO₄ for 1 h on ice, washed with PBS, and dehydrated with a graded series of ethanol solutions (50, 70, 90, 95, 99.5, and 100%) before exposure first to ethanol:propylenoxide (1:1, v/v) and then to 100% propylenoxide. The tissue was then exposed consecutively for 30 min to propylenoxide:epoxy resin (1:1, v/v), for 30 min to propylenoxide:epoxy resin (1:2, v/v), overnight to pure epoxy resin, and for 1 h to DMP-30. It was then embedded in epoxy resin for 2 days at 65 °C, cut at a thickness of 80 nm with the use of a Leica EM UC7 device, and stained with 2% uranyl acetate for 5 min before examination with a JEM-1400 Plus electron microscope (JEOL) at 80 kV and image capture with a CCD Veleta 2 K × 2 K camera (Olympus).