Hp 20ss

Column chromatography was done by using silica gel (60–120 and 100–200 mesh size), HP-20, HP-20SS, Sephedex, and silver nitrate-incorporated silica gel. Merck Kieselgel (Aufoilen) 60 F254 plates were used for thin-layer chromatography (TLC). High resolution mass spectra were obtained on Agilent 6540 (Q-TOF) mass spectrometer, in the electrospray (ESMS) mode. All solvents used for high performance liquid chromatography (HPLC) analysis were obtained from Merck Chemicals (Mumbai India). Water used for extraction and isolation purposes was obtained at Central Drug House (P) Ltd., Delhi. Spectroscopic data (NMR) of isolated compounds was gathered on a ¹H NMR at 400 and 500 MHz and on a¹³C NMR at 100 and 125 MHz (Bruker Advance spectrometer). The reference point was TMS (δ_H and δ_C: 0.00 ppm). Chemical shifts (δ) were referenced internally to the residual solvent peak (CD₃OD: ¹H- 3.30, ¹³C- 49.0 ppm). In 2D NMR, all heteronuclear ¹H and ¹³C correlations were established based on gradient-enhanced inverse-detected Heteronuclear Multiple Bond Correlation (HMBC) and Heteronuclear single quantum coherence (HSQC) experiments. HP-20, HP-20SS, Sephedex, silver nitrate, dimethylsulphoxide (DMSO), and all other chemicals were purchased from Sigma–Aldrich (St. Louis, MO, United States).

All chemicals were purchased from Sigma Aldrich (St. Louis, MO, USA) and Merck KGaA (Darmstadt, Germany). All solvents required for isolation and analytical experiments were purchased from Sigma Aldrich (St. Louis, MO, USA). The isolated compounds were dissolved in deuterated DMSO-d₆ and methanol-d₄ from Cambridge Isotope Laboratories (Tewksbury, MA, USA). Ultrapure water was produced with a Hydro^® (Hydro, NC, USA) purification system. HP-20 SS, Sephadex LH-20, and C18 silica (55–105 µm, 125 Å) material were purchased from Sigma-Aldrich (St. Louis, MO, USA), GE Healthcare (Chicago, IL, USA), and Waters (Milford, MA, USA), respectively.