Mts catalog no g111

IU1 (S7134), enzalutamide (S1250) and MK2206 (S1078) were obtained from Selleckchem (Houston, TX, USA). DMSO was used to dissolve these inhibitors and the inhibitors were stored at − 20 °C. USP14 (sc-76,817) siRNA was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). MTS (catalog no. G111) was obtained through Promega Corporation (Madison, WI, USA). Annexin V-FITC/PI apoptosis detection kits of Keygen Company (Nanjing, China)(KGA107) were purchased. Cell Lysis Buffer (#9803) was from Cell Signaling Technology (MA, USA) and stored at − 20 °C. Anti-GAPDH (MB001) was obtained from Bioworld Technology (St.Louis Park, MN, USA). The other antibodies were from Cell Signaling Technology (MA, USA): anti-PARP (#9542), anti-caspase 3 (#9668), anti-cleaved caspase 3 (#9661), anti-caspase 8 (#9746), anti-cleaved caspase 8 (#9496), anti-cleaved caspase 9 (#9501), anti-Bax (#5023), anti-CDK4(#12790), anti-P27(#3686), anti-caspase 9 (#9508), anti-CDK2 (#2546), anti-CyclinD1(#2922), anti-total-AKT (#9272), anti-AR (#5153), anti-USP14 (#11931), anti-Bcl-2 (#15071), anti-GSK3β (#12456), anti-p-GSK3β (Ser9)(#9323), anti-phospho-AKT (Ser473)(#4060), anti-β-catenin (#8480), anti-EGFR (#4267), anti-IGF1R (#9750).

MG132 (S2619), tamoxifen (S1238) were obtained from Selleck (Houston, TX, USA). Control siRNA (sc-37007) and USP15 siRNA (sc-76819) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). USP15-1 and USP15-2 siRNA (S0222) were from genechem (Shanghai, China). Cell-Light^TM EdU Apollo 567 In Vitro Kit (C10310-1) was obtained from RiboBio (Guangzhou, China). Annexin V-FITC/PI apoptosis detection kit (KGA107) was obtained from Keygen Company (Nanjing, China). The antibodies purchased from Cell Signaling Technology (CST, MA, USA) are listed below: anti-p21 (2947), anti-USP15 (66310), anti-ubiquitin (3936), anti-estrogen receptor α (8644), anti-phospho-Rb (8516), anti-Rb (9313), anti-Cyclin D1 (2922)(55506), anti-CDK4 (12790), anti-p27 (3686), anti-GAPDH (5174), anti-K48-ub (12805), anti-K63-ub (12930), anti-DYKDDDDK-tag (14793), anti-Myc-tag (2276). Anti-ERα (ab32063) was from Abcam (USA). Anti-USP15 (67557-1-lg) was from proteintech (Chicago, USA). Lentivirus was purchased from VigeneBio (Shandong, China) and plasmids were from Genechem (Shanghai, China). Cycloheximide (CHX) and Estrogen (E8875) were received from Sigma-Adrich (Sigma-Adrich, Louis, MO). MTS (catalog no. G111) was purchased from Promega Corporation (Madison, WI, USA). Co-IP assay kit (14311D) was purchased from Life Technologies (Carlsbad, CA).