H&E staining was performed as previously described (Guo et al., 2016 (link)). In brief, after deparaffinization and rehydration, 5-μm sections were stained with hematoxylin solution (G1120, Solarbio, Beijing, China) for 5 min at room temperature and washed twice with tap water (2 min/wash). The slides were then stained with eosin solution (G1120, Solarbio, Beijing, China) for 3 min at room temperature and washed twice with tap water (2 min/wash). Next, the slides were dehydrated in increasing concentrations of alcohol (75%, 85%, 95%, and 100% alcohol) for 2–3 s and rinsed in 100% alcohol for 1 min. The slides were then made transparent using xylene, sealed with glycerol gelatin aqueous slide mounting medium (S2150, Solarbio, Beijing, China), and mounted and photographed using an Olympus microscope (Olympus Corporation, Tokyo, Japan).
G1120
Manufactured by Solarbio
Sourced in China
G1120 is a high-precision electronic balance designed for laboratory use. It can accurately measure the weight of samples up to 120 grams with a readability of 0.1 milligram. The balance features a stainless steel weighing platform and a backlit LCD display for easy reading.
Automatically generated - may contain errors
Lab products found in correlation
G1340, by Solarbio (7 mentions)
G1346, by Solarbio (5 mentions)
Dm1000 led, by Leica (3 mentions)
Da1010, by Solarbio (3 mentions)
G8590, by Solarbio (2 mentions)
G1436, by Solarbio (2 mentions)
G1371, by Solarbio (2 mentions)
Masson s trichrome staining, by Solarbio (2 mentions)
Safranin o fast green, by Solarbio (2 mentions)
G1281, by Solarbio (2 mentions)
Masson s trichrome, by Solarbio (2 mentions)
Pk10006, by Solarbio (2 mentions)
Ass3403, by Abcepta (2 mentions)
W11261, by Thermo Fisher Scientific (2 mentions)
G1472, by Solarbio (2 mentions)
Dm3000, by Leica (2 mentions)
He staining kit, by Solarbio (2 mentions)
S2150, by Solarbio (1 mentions)
P6148, by Merck Group (1 mentions)
Diaphot tmd, by Nikon (1 mentions)
94 protocols using g1120
1
Hematoxylin and Eosin Staining Protocol
2
Histopathological Analysis of Lung, Liver, and Heart
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Lung, liver and heart tissues fixed with 4% paraformaldehyde (P6148, Sigma) were cut and dehydrated. These tissues were then waxed into wax blocks. The blocks were cut into 5 μm slices by slicer and then dewaxed, rehydrated and stained for 3 min with haematoxylin (G1120, Solarbio, Beijing, China). Following, the lung, liver and heart sections were differentiated with 1% hydrochloric acid alcohol for 20 s and were stained for 1 min with eosin (G1120, Solarbio). After washing, these tissues were dehydrated with ethanol solution of different concentrations (80%, 90%, 95%, 100%) at room temperature. After soaking in xylene for 10 min, the sections were sealed with neutral gum. The pathological alterations were observed and photographed by an optical microscope (DIAPHOT-TMD, Nikon, Osaka, Japan). The scoring standard of inflammatory infiltration was almost invisible marking 0, mild inflammatory infiltration marking 1, moderate inflammatory infiltration marking 3, and severe inflammatory infiltration marking 5.
3
Paraffin-embedded Tissue Staining Protocol
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After the paraffin sections were prepared, they were heated in a constant temperature oven at 60 °C for 4 h. Then, the sections were placed in xylene I and II for 10 min and placed in alcohol solutions with different concentrations, including 100%, 95%, 90%, 80%, and 70%, for 5 min each time, and rinsed in distilled water for 1 min. The sections were stained with the hematoxylin (Solarbio, #G1120, Beijing, China) staining solution for 2 min, differentiated with the differentiation solution (1% of hydrochloric acid), washed in distilled water again for 2 min, and then dyed with 0.5% of the eosin staining solution (Solarbio, #G1120, Beijing, China) for 1 min. Finally, in order to complete the dehydration, the tissue sections were successively placed in 70%, 80%, 90%, and 100% gradient ethanol solution. The slides were placed in xylene I and II for 5 min and were sealed with neutral resin (Solarbio, # G8590, Beijing, China). Samples were observed and photographed on a fully automated tissue in situ multi-label landscape quantification analyzer (Vectra Polaris, PerkinElmer).
4
Histological Sample Preparation and Staining
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Organs were fixed overnight in 4% of paraformaldehyde. The samples were removed and dehydrated using a gradient of ethanol and xylene. After embedding the samples in paraffin overnight, paraffin sections were prepared using a rotary microtome. The sections were kept at 65°C for 15 min, followed by a rinse in xylene for 10 min, this step was repeated in fresh xylene, and the sections were rehydrated using gradient ethanol. The sections were rinsed with hematoxylin (Solarbio, G1120) for 10 s, destained in acidic ethanol, and rinsed in tap water. The sections were stained with eosin (Solarbio, G1120) for 30 s and transferred to distilled water. The dehydration step was repeated, and the sections were mounted with sufficient neutral balsam and examined with a slice scanning machine.
5
Tissue Preparation for Histological Analysis
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The embedded tissues were cut into 3 μm, then dewaxed with xylene (2 times, 5 min each time), and hydrated with graded ethanol (ethanol, 5 min; 95% ethanol, 2 min; 80% ethanol, 2 min; 70% ethanol, 2 min). After washing with water for 2 min, the sections were stained with hematoxylin staining (G1120, Solarbio, Beijing, China) for 15 min, then differentiated with differentiation liquid for 30 s. After soaking for 15 min in water, the sections were stained with eosin (G1120, Solarbio, Beijing, China) for 50 s. After washing and soaking with water, the sections were dehydrated with graded ethanol (95% ethanol, 2 s; 95% ethanol, 2 s; 100% ethanol, 2 s; 100% ethanol, 1 min;) and purified with xylene (2 times, 1 min each time). At least, the sections were sealed with neutral gum and observed under a light microscope (DM1000 LED, Leica, Germany).
6
Histological Assessment of Mouse Brain Injury
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Four mice in each group were anesthetized by intraperitoneal injection of 10% chloral hydrate, and the heart was perfused with 20 mL of normal saline and 20 mL of 4% paraformaldehyde. The brain was completely removed, dehydrated, waxed, embedded, and serial coronal sections (8 μm) patch spared. The paraffin sections were taken, deparaffinized and hydrated, stained with hematoxylin (G1120, Solarbio, CN) for 3 min, differentiated for 10 s, soaked in tap water for 15 min, stained with eosin (G1120, Solarbio, CN) for 1 min, and the color development was stopped in tap water. Conventional dehydrated, transparent, and neutral balsam seals were applied.The hippocampus structure was observed under a light microscope, and the nucleus and the cytoplasm showed blue and red, respectively. Five non-overlapping fields on each slice were recorded by optical microscope (IX70, OLYMPUS, JPN). The degree of damage is expressed by the denatured cell index (DCI, number of denatured cells/total number of cells).
7
Cryopreservation and Sectioning of Tissue Samples
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Spare tissue samples were removed from −80°C, thawed in equilibrium at 4°C, placed in 4% paraformaldehyde solution and fixed at 4°C overnight. The next day, the fixed tissues were washed three times with PBS for 3 min each time. After the filter paper dried, the tissues were placed in 30% sucrose solution and dehydrated overnight at 4°C until the tissues sank to the bottom. The excess water was absorbed with filter paper, the appropriate angle was adjusted according to the sectioning direction, and the tissue was placed on the precooled section substrate; then, the tissues were embedded with OTC embedding agent before cryosectioning. The microtome was prechilled, and the tissues were sectioned after being completely frozen. The sections were stained using a hematoxylin eosin (HE) staining kit (G1120, Solarbio) according to the instructions.
8
Histological Staining Protocols for Tissue Analysis
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Hematoxylin and eosin (HE) staining (G1120, Solarbio, China), Safranin O-fast green (SO-FG) staining (Servicebio, China), and Sirius red staining (G1018, Servicebio, China) were carried out following the manufacturer's procedures. A Pannoramic MIDI scanner (3DHISTECH, Hungary) was used to capture images.
9
Multimodal Histological Staining Protocol
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Histological staining with hematoxylin and eosin (H&E), Nissl, and Luxol® fast blue (LFB) was conducted using commercial kits (G1120, G1436, G3240; Beijing Solarbio Science and Technology Co., Ltd., Beijing, China) in accordance with the manufacturer’s protocols. Sections were mounted with neutral balsam (G8590; Beijing Solarbio Science and Technology Co., Ltd.) and then photographed using a fluorescence microscope.
10
Histological Analysis of Tissue Samples
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Tissue samples of NSs and HSs were trimmed to remove subcutaneous adipose tissue and then fixed in 4% paraformaldehyde, embedded in paraffin, sectioned into 5-μm thickness (UC7, Leica), mounted on slides, and performed HE staining (G1120, Solarbio) and MASSON staining (G1346, Solarbio) following the instructions. Images were acquired with an inverted phase contrast microscope (HY7800, Hitachi).
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