Pen strep mix

Manufactured by Thermo Fisher Scientific

Sourced in United States

Pen/Strep mix is a sterile solution containing penicillin and streptomycin antibiotics. It is commonly used in cell culture media to prevent bacterial contamination.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Sodium pyruvate, by Merck Group (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Expi293f cells, by Thermo Fisher Scientific (2 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (2 mentions) Erlenmeyer cell culture flasks, by Corning (2 mentions) Expi293 expression medium, by Thermo Fisher Scientific (2 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Bacterial collagenase, by Merck Group (1 mentions) Caspase 9 total and cleaved, by Cell Signaling Technology (1 mentions) Caspase 7 total and cleaved, by Cell Signaling Technology (1 mentions) Mcf 7 cells atcc htb 22, by American Type Culture Collection (1 mentions) Mem eagle s medium, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase conjugated anti rabbit igg and anti mouse igg antibodies, by Merck Group (1 mentions) Cox 2, by Cell Signaling Technology (1 mentions) P ampkα, by Cell Signaling Technology (1 mentions) Caspase 8, by Cell Signaling Technology (1 mentions) Beclin 1, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Prodh pox, by Santa Cruz Biotechnology (1 mentions)

10 protocols using pen strep mix

Molecular Mechanisms of MCF-7 Cell Apoptosis

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MCF-7 cells (ATCC^® HTB-22™) were obtained from ATCC (Manassas, VA, USA). MEM Eagle’s medium, fetal bovine serum, puromycin, Pen/Strep mix, and PBS were products of Gibco (Waltham, MA, USA). Propidium iodide (PI), annexin V-CF488A conjugate, and annexin V binding buffer were provided by the Biotium Company (Fremont, CA, USA). NC-Slide A2™ was purchased from Chemometec (Allerod, Denmark). Horseradish peroxidase conjugated anti-rabbit IgG and anti-mouse IgG antibodies, bacterial collagenase, 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′,7′-dichlorofluorescin diacetate (DCFDA), indomethacin, and diclofenac were purchased from Sigma Aldrich (Saint Louis, MO, USA). Primary antibodies against COX2, p-AMPKα, mTor, caspase 8, caspase 9 total and cleaved, caspase 7 total and cleaved, BID, PARP, Beclin 1, AGT5, ATG 7, and B-actin were products of Cell Signaling Technology (Danvers, MA, USA). Primary antibodies for PRODH/POX, PPARγ, GLUD 1/2, and prolidase were products of Santa Cruz Biotechnology (Dallas, TX, USA). PYCR1, PYCRL, and PPARδ primary antibodies were obtained from Abnova (Taipei, Taiwan). Hoechst 33342 was obtained from Becton Dickinson (Franklin Lakes, NJ, USA). CRISPR All-In-One Non-Viral Vector and lipofectamine were products of Applied Biological Materials Inc. (Richmond, BC, Canada).

Kazberuk A., Chalecka M., Palka J., Bielawska K, & Surazynski A. (2022). NSAIDs Induce Proline Dehydrogenase/Proline Oxidase-Dependent and Independent Apoptosis in MCF7 Breast Cancer Cells. International Journal of Molecular Sciences, 23(7), 3813.

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SH-SY5Y Neuroblastoma Metabolic Responses

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SH-SY5Y neuroblastoma cells were grown in DMEM-F12 media (Gibco - #11320-074) supplemented with 10% FBS (Gibco - #3000008085) and 1% Pen/Strep mix (Gibco - #15140122), at 37 °C with 5% CO2. Cells were maintained in T75 flasks, and media was changed every 2 days. For the experiments, cells were subcultured in different dish formats (6/24 wells plates) and used 36 h after seeding. Sodium L-lactate (Sigma - #71718) and sodium pyruvate (Sigma - #P2256) were used in this study to investigate the role of both metabolites in cell survival.
For pharmacological treatments, inhibitors (AR-C155858 – Tocris #4960, LY294002 – Sigma #L9908, Quercetin – Sigma #00200595, Cycloheximide – Sigma #C1988 and N-acetylcysteine – Sigma #A7250) were applied 15 min before lactate or pyruvate stimulation, and all treatments were conducted in DMEM-F12 media supplemented with 10% FBS.

Tauffenberger A., Fiumelli H., Almustafa S, & Magistretti P.J. (2019). Lactate and pyruvate promote oxidative stress resistance through hormetic ROS signaling. Cell Death & Disease, 10(9), 653.

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Biospecimen Handling for HNSCC Research

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All HNSCC tumor samples were obtained from patients, with their informed consent, at Montefiore Medical Center and all procedures were executed under Institutional Review Board (IRB) guidelines of Montefiore Medical Center and the Albert Einstein College of Medicine (IRB #2018–8778). All experimental methods were performed in accordance with relevant guidelines and regulations.
Tumor samples obtained from operative biopsies or resections were collected under the guidance of the pathologist on-call to ensure that removal of the research specimen would not affect diagnosis/margin assessment. Samples were briefly washed in 100% ethanol followed by sterile PBS, before being placed into a 15-ml conical tube containing sterile Dulbecco’s Modified Eagle’s Media (DMEM) with 1% Pen/Strep mix (Gibco, cat. No. 15140–122); Gentamicin (Gibco, cat. No 15710–064) at a final concentration of 40 μg/ml; Amphotericin B (Fisher Scientific, cat. No. BP264550) at a final concentration of 0.5 μg/ml, and Nystatin (Sigma-Aldrich, cat. No. N6261) at final concentration of 0.1 mg/ml. Samples were transported on ice from the pathology department to the laboratory. Matched adjacent tumor samples were also obtained and flash frozen in liquid nitrogen to be maintained as reference tumor.

Li D., Thomas C., Shrivastava N., Gersten A., Gadsden N., Schlecht N., Kawachi N., Schiff B.A., Smith R.V., Rosenblatt G., Augustine S., Gavathiotis E., Burk R., Prystowsky M.B., Guha C., Mehta V, & Ow T.J. (2023). Establishment of a diverse head and neck squamous cancer cell bank using conditional reprogramming culture methods. Journal of medical virology, 95(2), e28388.

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Culturing Mouse Microglia N9 Cells

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N9 mouse microglia were a kind gift from Professor Dora Brites (iMed.ULisboa). Cells were cultured in RPMI 1640 medium (Gibco, Waltham, USA) supplemented with 10% FBS (Biochrom AG, Berlin, Germany) and 1% Pen-Strep Mix (Gibco). Cells were kept at 37 °C in a humidified incubator under a 5% CO₂ atmosphere. For passing, plating or collection of cells from plates, the medium was removed, and the cells were rinsed with Versene (Gibco) and incubated at 37 °C with 0.05% Trypsin-EDTA (Gibco) for 10 min, after which culture medium was added to inhibit trypsin.

Branco V., Coppo L., Aschner M, & Carvalho C. (2022). N-Acetylcysteine or Sodium Selenite Prevent the p38-Mediated Production of Proinflammatory Cytokines by Microglia during Exposure to Mercury (II). Toxics, 10(8), 433.

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Bacterial Growth in Cell Culture

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Bacterial strains used in this study are listed in Table S1 in the supplemental material. Experiments were performed in lysogeny broth (LB) or RPMI medium enriched with 10% fetal bovine serum (FBS). Culture media have been supplemented or not with penicillin-streptomycin (Pen-Strep) mix (200 U/mL; Thermo Fisher; catalog no. 15140122).

Znaidia M., de Souza-Angelo Y., Létoffé S., Staropoli I., Grzelak L., Ghigo J.M., Schwartz O, & Casartelli N. (2023). Exposure to Secreted Bacterial Factors Promotes HIV-1 Replication in CD4+ T Cells. Microbiology Spectrum, 11(2), e04313-22.

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Bacterial Growth in Cell Culture

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Cell Line Culture Protocols

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Human embryonic kidney 293T (293T), SupT1, THP-1 and Jurkat cell lines were obtained from the American Type Culture Collection. THP-1 MyD88-/- cells were obtained from InVivoGen. 293T were cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (ThermoFisher Scientific), 1% HEPES (Corning), 1% nonessential amino acids (Sigma-Aldrich), 1% sodium pyruvate (Sigma-Aldrich) and 2% PenStrep mix (ThermoFisher Scientific). THP-1, THP-1 MyD88^-/-, SupT1 and Jurkat cell lines were cultured in RPMI 1640 (ThermoFisher Scientific) supplemented with 10% HI-FBS and 1% HEPES.

Iampietro M., Younan P., Nishida A., Dutta M., Lubaki N.M., Santos R.I., Koup R.A., Katze M.G, & Bukreyev A. (2017). Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection. PLoS Pathogens, 13(5), e1006397.

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Influenza Virus Propagation in MDCK and Expi293F Cells

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Madin-Darby canine kidney (MDCK) cells (American Type Culture Collection; CCL-34) were maintained at 37°C/5% CO₂ in minimum essential medium (MEM; Life Technologies, Carlsbad, California, U.S.A.) containing 10% fetal bovine serum (Thermo Fisher Scientific, Grand Island, New York, U.S.A.) and pen-strep mix (100 units/ml penicillin and 100 μg/ml streptomycin; Life Technologies). Expi293F cells (Thermo Fisher Scientific) were maintained at 37°C/8% CO₂ in Erlenmeyer cell culture flasks (Corning, Tewksbury, Massachusetts, U.S.A.) containing Expi293 Expression Medium (Thermo Fisher Scientific). A/California/7/2009 (H1N1)pdm09 (X-179A), A/Puerto Rico/8/1934 (H1N1), A/New Caledonia/20/1999 (H1N1), A/New York/55/2004 (H3N2), A/Victoria/210/2009 (H3N2), and mouse-adapted A/Narita/1/2009 (H1N1)pdm09 viruses were grown in 10 or 11-day-old embryonated chicken eggs. A/Narita/1/2009 (H1N1)pdm09 virus and A/Narita/1/2009 (H1N1)pdm09-derived F11 escape mutants were propagated in MDCK cells.

Sano K., Saito S., Suzuki T., Kotani O., Ainai A., van Riet E., Tabata K., Saito K., Takahashi Y., Yokoyama M., Sato H., Maruno T., Usami K., Uchiyama S., Ogawa-Goto K, & Hasegawa H. (2021). An influenza HA stalk reactive polymeric IgA antibody exhibits anti-viral function regulated by binary interaction between HA and the antibody. PLoS ONE, 16(1), e0245244.

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Culturing MDCK, hCK, and Expi293F cells

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Madin-Darby canine kidney (MDCK) cells (American Type Culture Collection; CCL-34) were maintained at 37 °C/5% CO₂ in minimum essential medium (MEM; Life Technologies, Carlsbad, CA, USA) containing 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) and pen-strep mix (100 units/mL penicillin and 100 μg/mL streptomycin; Life Technologies). Humanized MDCK (hCK) cells [47 (link)] were maintained at 37 °C/5% CO₂ in Dulbecco’s Modified Eagle’s Medium-high glucose (DMEM; Sigma-Aldrich, St. Louis, MO, USA) containing 5% fetal bovine serum, pen-strep mix (100 units/mL penicillin and 100 μg/mL streptomycin), 5 µg/mL blasticidin (InvivoGen, San Diego, CA, USA), and 2 µg/mL puromycin dihydrochloride (Life Technologies). Expi293F cells (Thermo Fisher Scientific) were maintained at 37 °C/8% CO₂ in Erlenmeyer cell culture flasks (Corning, Tewksbury, MA, USA) containing Expi293 Expression Medium (Thermo Fisher Scientific). A/Narita/1/2009 (H1N1)pdm09 virus [19 (link)] and A/Narita/1/2009 (H1N1)pdm09-derived F11 escape mutants (C1 and G6 [18 (link)]) were propagated in MDCK cells.

Kotani O., Suzuki Y., Saito S., Ainai A., Ueno A., Hemmi T., Sano K., Tabata K., Yokoyama M., Suzuki T., Hasegawa H, & Sato H. (2021). Structure-Guided Creation of an Anti-HA Stalk Antibody F11 Derivative That Neutralizes Both F11-Sensitive and -Resistant Influenza A(H1N1)pdm09 Viruses. Viruses, 13(9), 1733.

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Alzheimer's Disease Cell Line Protocols

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CHO cell lines stably overexpressing WT APP (7 W cells) (Koo and Squazzo, 1994 (link)) and stably overexpressing WT APP and WT PS1 (PS70 cells) (Xia et al., 1997 (link)) were a kind gift from Dr. Dennis Selkoe (Brigham and Women’s Hospital, Harvard Medical School, USA). PS1/PS2 double knockout mouse embryonic fibroblasts (PS1/2 dKO MEF) were a kind gift from Dr. Bart De Strooper (Katholieke Universiteit Leuven, Leuven, Belgium). These cells were authenticated using STR profiling, monitored for mycoplasma contamination every 2 months, and maintained as described previously (Uemura et al., 2010 (link)). Lipofectamine 3000 (Life technologies, Carlsbad, CA, USA) was used for transient transfection according to the manufacturer’s instructions. Primary neuronal cultures were obtained from cerebral cortex and hippocampus of mouse embryos at gestation day 14–16 (Charles River Laboratories, Wilmington, MA). The neurons were dissociated using Papain Dissociation System (Worthington Biochemical Corporation, Lakewood, NJ, USA) and were maintained for 13–15 days in vitro (DIV) in Neurobasal medium containing 2% B27 supplement, 1% GlutaMax, and 1% Pen/Strep mix (Life Technologies).

Maesako M., Horlacher J., Zoltowska K.M., Kastanenka K.V., Kara E., Svirsky S., Keller L.J., Li X., Hyman B.T., Bacskai B.J, & Berezovska O. (2017). Pathogenic PS1 phosphorylation at Ser367. eLife, 6, e19720.

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