Genomic DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). FOLR1 sequence was subsequently amplified via PCR using Q5 Hot Start High-Fidelity 2X Mastermix (New England Biolabs, Ipswich, MA, USA) and FOLR1 flanking primer pair (forward primer sequence: 5′-AGGGAGGGGTGGTGTCTAAT-3′; reverse primer sequence: 5′-CCTTTGGGCCTGCTTCCTTA-3′ (Metabion, Planegg, Germany). PCR product was afterward cleaned via the NucleoSpin Gel and PCR Clean-Up Kit (Qiagen). The purified PCR product was sent to Eurofins genomics for sequencing.
Nucleospin gel and pcr clean up kit
Manufactured by Qiagen
Sourced in China
The NucleoSpin Gel and PCR Clean-Up Kit is a laboratory product designed to purify DNA fragments from agarose gels and remove primers, nucleotides, salts, and other contaminants from PCR reactions. The kit utilizes a silica-membrane technology to efficiently capture and purify DNA samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using nucleospin gel and pcr clean up kit
1
FOLR1 Gene Amplification and Sequencing
2
High-throughput Small RNA Sequencing
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Small RNA library preparation for high-throughput sequencing was carried out using a NEBNext Small RNA Library Prep Set for Illumina (New England BioLabs, Inc., Ipswich, MA, USA).Thereafter, the small RNA library was purified using a NucleoSpin Gel and PCR Clean-up kit (Qiagen, Shanghai, China). The resulting purified library was analyzed for purity on an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA) and then subjected to single-ended, strand-specific sequencing on an Illumina HiSeq X10 platform.
3
Small RNA Sequencing Library Preparation
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Small RNA library preparation for high throughput sequencing was performed using the protocols and reagents from the NEBNext Small RNA Library Prep Set for Illumina (New England BioLabs, Inc., Ipswich, MA, USA). The small RNA library prepared from PDCs was fractionated by DNA length on a 2% TAE agarose gel for the excision of the 150-bp band. The small RNA library was purified using NucleoSpin Gel and PCR Clean-Up kit (Qiagen, Shanghai, China). The purified library was analyzed for purity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA) and then subjected to single-ended strand-specific sequencing on the Illumina HiSeq X10 platform (Illumina, Inc., San Diego, CA, USA).
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