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Ad 7013mzt

Manufactured by Dino-Lite

The AD-7013MZT is a digital handheld microscope from Dino-Lite. It features a CMOS sensor with 5.0 megapixel resolution and provides up to 200x magnification. The device is equipped with LED illumination and can capture still images and videos.

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6 protocols using ad 7013mzt

1

Fertilized Egg Collection Protocol

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The water of breeding tank was made by mixing rearing water and purified water by reverse osmosis, then was treated with peat moss for cultivation. It was adjusted to 24 ± 0.5 °C, 30 ppm, and pH 5.0 ± 0.5, respectively. One day prior to the collection of fertilized eggs, male and female of experimental fishes in a 2 to 1 ratio were put into a glass water tank (45X30X30cm) with a net. The fertilized egg was corrected at the bottom of glass water tank after spawning. Fertilized eggs which confirmed the formation of perivitelline space were measured for size (n = 20) under digital microscope (AD-7013MZT, Dino-Lite, Anmo, Taiwan) and used in this study as experimental samples.
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2

Cultivation and Measurement of Fertilized Fish Eggs

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The water of breeding glass water tank (45X30X30 cm) was made by mixing rearing water and purified water by reverse osmosis, then was treated with peat moss for cultivation. It was adjusted to 25 ± 0.5 °C, 80 ppm, and pH 6.0 ± 0.5, respectively. The flower clay pot was used as a spawning ground. The fertilized egg mass was isolated using stainless chopsticks being careful not to break the fertilized eggs. Fertilized eggs which confirmed the formation of perivitelline space were measured for size (n = 20) under digital microscope (AD-7013MZT, Dino-Lite, Anmo, Taiwan) and used in this study as experimental samples.
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3

Electrical and Microwave Measurements of Nanodevices

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To obtain electrical and microwave access to the nanodevice and then perform measurements, we used a custom-built probe station, where we mounted the sample with a fixed in-plane angle on top of a variable field module v.2 (VFM2)48 . VFM2 uses permanent magnets (rather than electromagnetic coils) to avoid heating during ramping of the field and the associated drift and can apply in-plane magnetic fields of more than ±0.8 Tesla (8000 G) while offering a ∼1 G field resolution.
Moreover, a microscope was needed to make precise contact between the microwave probe and the sample. Therefore, for the vision part of the sample holder, we employed a Dino-Lite AD7013MZT digital hand-held microscope camera with a 5 megapixel sensor and an adjustable polarizer with up to x240 magnification on a fine-focus adjustment stand.
A direct electric current was applied to the devices through a high-frequency bias-T, and the resulting rf oscillations were amplified by a low-noise amplifier and recorded with a high-frequency spectrum analyzer (SA) using a low-resolution bandwidth of 1 MHz. The auto-oscillations were detected electrically in the tens of devices measured, and the results were reproducible from device to device.
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4

Breeding and Egg Morphology of Aquarium Fish

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For breeding, 20 adult fish were selected and moved to a glass tank (60 × 30 × 35 cm). The water used for breeding was treated with peat moss and maintained at 25 ± 0.5 °C and pH 6.0 ± 0.5. A bundle of plastic strings (each 30 cm in length) was used as a spawning substrate. The fertilized egg was isolated using fingers, while being careful not to break the fertilized eggs. The diameter of fertilized eggs confirmed to have perivitelline space (n = 30) were measured using a digital microscope (AD-7013MZT, Dino-Lite, Anmo, Taiwan) and used for morphological analyses.
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5

Acrylic Yarn Spawning Ground

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It used the same rearing water as water of breeding tank (45X30X30 cm) and a bundle of acrylic knitting yarn was used as a spawning ground. The fertilized eggs were isolated using fingers being careful not to break the fertilized eggs from a bundle of acrylic knitting yarn. Fertilized eggs that confirmed the formation of perivitelline space were measured for size (n = 30) under digital microscope (AD-7013MZT, Dino-Lite, Anmo, Taiwan) and used in this study as experimental samples.
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6

Visualizing DMNR-Induced Skin Penetration

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To further observe the holes created of DMNR visually, Parafilm M ® , a validated skin model (39) , was used as an artificial skin simulants. The Parafilm M ® sheet was folded to get an eight-layer film (appromiximately127 μm thickness per layer). Following, SRB-loaded DMNR (375 DMNs, 600 μm) was installed on a homemade applicator and rolled forward once across the eight-layer film row by row. After insertion, the sheet was unfolded and observed using a handheld microscope (AD7013MZT, Dino-lite, Taiwan, China). The number of holes created in each layer by DMNRs were then calculated under microscope after washing off the fractured MNs in the sheet.
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