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2 protocols using α cd63

1

Antibody Characterization for Western Blot

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All antibodies used in this study are listed here, and concentrations used for specific experiments are listed in the relevant subsections as follows: α-actin (Millipore, MAB1501R), α-actin (Cytoskeleton Inc., AAN02), α-ALDH1L1 (Abcam, ab177463), α–annexin A1 (Abcam, ab214486), α-calnexin (BD Biosciences, 610523), α-CD63 (Santa Cruz Biotechnology, 5275), α-CD81 (BD Biosciences, 555675), α-FLAG (Millipore, F7425), α-FLAG-M2 (Millipore, F1804), α-GAPDH (α–glyceraldehyde-3-phosphate dehydrogenase; Cell Signaling Technology, 8884), α-GFAP (Millipore, MAB360), α-GFAP (DAKO, Z0334), α-GLAST (39 (link)), α-GLT1 (39 (link)), α-MCT4 (Proteintech, 22787-1-AP), α-NeuN (Millipore, MAB377), α-RFP (α–red fluorescent protein; Abcam, ab62341), α-S100β (DAKO, Z0311), α-WAVE2 (Cell Signaling Technology, 3659), and α-WAVE3 (Cell Signaling Technology, 2806).
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2

Western Blot Antibody Profiling for HTLV-1 Proteins

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Primary antibodies against proteins of interest used in Western blot assays were: α-Tax (Tax antibodies 169, 170, and 171 monoclonal mouse (generous gift of Dr. Scott Gitlin, University of Michigan) [35 (link), 54 (link)]; α-p19 (Santa Cruz Biotechnology, sc-1665); α-gp61/46 (NIH AIDS Reagent Program Cat. # 1578); LC3-1/LC3-II (Santa Cruz Biotechnology, Cat. # SC-398822); p62 autophagy marker (Cell Signaling Inc., Cat. # 5114S); α-CD45 (Santa Cruz Biotechnology, Cat. # SC-1123); α-CD43 (Santa Cruz Biotechnology, Cat. # SC-70682); α-ICAM-1 (Santa Cruz Biotechnology, Cat. # SC-8439); α-LFA-1 (Santa Cruz Biotechnology, Cat. # SC-374172); α-CD63 (Santa Cruz Biotechnology, Cat. # SC-365604); α-CD63 tetraspanin marker (SBI System Biosciences, Cat. # EXOAB-CD63A-1); α-histone H2B (Cell Signaling Technology, Cat. # 12,364); α-histone H2A (Santa Cruz Biotechnology, Inc., Cat. # SC-10807 and abcam, Cat. # ab18255); α-histone H3 (Santa Cruz Biotechnology, Inc., Cat. # SC-10809); α-histone H4 (Cell Signaling Technology, Cat. # 2592); α-histone H1 (GeneTex, Cat. # GTX114462); IL-8 (Santa Cruz Biotechnology, Cat. # SC-376750); IL-6 (Santa Cruz Biotechnology, Cat. # SC-28343); RANTES (Santa Cruz Biotechnology, Cat. # SC-514019); GAPDH (Santa Cruz Biotechnology, Cat. # SC-48166); and α-Actin (Abcam; ab469900).
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