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P38 inhibitor 4

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The P38 inhibitor IV is a laboratory reagent that inhibits the activity of the p38 mitogen-activated protein kinase (MAPK) signaling pathway. It is used in research applications to study the role of the p38 MAPK pathway in cellular processes.

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Lab products found in correlation

Recombinant tgf β1, by R&D Systems (4 mentions) Anti cd3, by BioLegend (4 mentions) Anti cd28, by BioLegend (4 mentions) Anti il 4 11b11, by BioLegend (2 mentions) Mp5 20f3, by BioLegend (2 mentions) Anti il 6 mab, by BioLegend (2 mentions) Pd98059, by Merck Group (2 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (2 mentions) Anti il 4 clone, by BioLegend (2 mentions) Anti il 6 mab mp5 20f3, by BioLegend (2 mentions) Mek inhibitor pd98059, by Merck Group (2 mentions) Salirasib, by Merck Group (1 mentions) Pd184352, by Selleck Chemicals (1 mentions)

5 protocols using p38 inhibitor 4

1

Replication Assays and Western Blotting

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For replication assays, cells were seeded in 24-well plates at 1 × 105 cells/well and for western blotting in 60 mm dishes at 2 × 105 cells/dish. Plates were then treated with one of the following inhibitors; salirasib [10 μM] (Sigma), p38 inhibitor IV [10 μM] (Sigma) or PD 184352 [1 μM] (Selleck), 1 hour before irradiation at 5 Gy. Cells were infected with RT3D (MOI 5) 4 hours after irradiation. One-step growth curves and western blotting were carried out as described.
McEntee G., Kyula J.N., Mansfield D., Smith H., Wilkinson M., Gregory C., Roulstone V., Coffey M, & Harrington K.J. (2016). Enhanced cytotoxicity of reovirus and radiotherapy in melanoma cells is mediated through increased viral replication and mitochondrial apoptotic signalling. Oncotarget, 7(30), 48517-48532.
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2

Induced iTreg Cell Differentiation

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Sorted naive CD4+CD62L+Foxp3EGFP− T cells (1 × 106/ml) were cultured with plate-bound anti-CD28 (5 μg/ml, Biolegend), anti-CD3 (5 μg/ml, Biolegend), recombinant TGF β1 (5 ng/ml, R&D Systems), with or without murine recombinant IL-4 (10 ng/ml) (Perpotech), anti-IL-4 (11B11, 10 μg/ml) (Biolegend) or anti-IL-6 mAb (MP5-20F3, 10 μg/ml) (Biolegend), mitogen activated protein kinase kinase (MEK) inhibitor PD98059 (50 μM, Sigma-Aldrich) or P38 inhibitor IV (10 μM, Sigma-Aldrich). After 4 days, the induced iTreg cells were analyzed by flow cytometry for Foxp3 expression and intracellular cytokines production and/or re-sorted on the basis of EGFP fluorescence.
Massoud A.H., Charbonnier L.M., Lopez D., Pellegrini M., Phipatanakul W, & Chatila T.A. (2016). An asthma-associated IL4R variant exacerbates airway inflammation by promoting conversion of regulatory T cells to TH17-like cells. Nature medicine, 22(9), 1013-1022.
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3

Induction and Characterization of Induced T-regulatory Cells

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Sorted naïve CD4+CD62L+Foxp3YFPCre T cells (1 × 106/ml) were cultured with plate-bound anti-CD28 (5 μg/ml, Biolegend), anti-CD3 (clone: 145–2C11, 5 μg/ml, Biolegend), recombinant TGF-β1 (5 ng/ml, R&D Systems), with or without IL-4 (10 ng/ml) (Peprotech), anti-IL-4 (clone: 11B11, 10 μg/ml) (Biolegend) or anti-IL-6 mAb (MP5–20F3, 10 μg/ml) (Biolegend), mitogen activated protein kinase (MEK) inhibitor PD98059 (50 μM, Sigma-Aldrich) or P38 inhibitor IV (10 μM, Sigma-Aldrich). After 4 days, the induced Treg cells were analyzed by flow cytometry for Foxp3 expression and intracellular cytokines production and/ re-sorted on the basis of YFP expression.
Harb H., Stephen-Victor E., Crestani E., Benamar M., Massoud A., Cui Y., Charbonnier L.M., Arbag S., Baris S., Cunnigham A., Leyva-Castillo J.M., Geha R.S., Mousavi A.J., Guennewig B., Schmitz-Abe K., Sioutas C., Phipatanakul W, & Chatila T.A. (2020). A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma. Nature immunology, 21(11), 1359-1370.
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4

Induction and Characterization of Induced T-regulatory Cells

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Sorted naïve CD4+CD62L+Foxp3YFPCre T cells (1 × 106/ml) were cultured with plate-bound anti-CD28 (5 μg/ml, Biolegend), anti-CD3 (clone: 145–2C11, 5 μg/ml, Biolegend), recombinant TGF-β1 (5 ng/ml, R&D Systems), with or without IL-4 (10 ng/ml) (Peprotech), anti-IL-4 (clone: 11B11, 10 μg/ml) (Biolegend) or anti-IL-6 mAb (MP5–20F3, 10 μg/ml) (Biolegend), mitogen activated protein kinase (MEK) inhibitor PD98059 (50 μM, Sigma-Aldrich) or P38 inhibitor IV (10 μM, Sigma-Aldrich). After 4 days, the induced Treg cells were analyzed by flow cytometry for Foxp3 expression and intracellular cytokines production and/ re-sorted on the basis of YFP expression.
Harb H., Stephen-Victor E., Crestani E., Benamar M., Massoud A., Cui Y., Charbonnier L.M., Arbag S., Baris S., Cunnigham A., Leyva-Castillo J.M., Geha R.S., Mousavi A.J., Guennewig B., Schmitz-Abe K., Sioutas C., Phipatanakul W, & Chatila T.A. (2020). A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma. Nature immunology, 21(11), 1359-1370.
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5

Induced iTreg Cell Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sorted naive CD4+CD62L+Foxp3EGFP− T cells (1 × 106/ml) were cultured with plate-bound anti-CD28 (5 μg/ml, Biolegend), anti-CD3 (5 μg/ml, Biolegend), recombinant TGF β1 (5 ng/ml, R&D Systems), with or without murine recombinant IL-4 (10 ng/ml) (Perpotech), anti-IL-4 (11B11, 10 μg/ml) (Biolegend) or anti-IL-6 mAb (MP5-20F3, 10 μg/ml) (Biolegend), mitogen activated protein kinase kinase (MEK) inhibitor PD98059 (50 μM, Sigma-Aldrich) or P38 inhibitor IV (10 μM, Sigma-Aldrich). After 4 days, the induced iTreg cells were analyzed by flow cytometry for Foxp3 expression and intracellular cytokines production and/or re-sorted on the basis of EGFP fluorescence.
Massoud A.H., Charbonnier L.M., Lopez D., Pellegrini M., Phipatanakul W, & Chatila T.A. (2016). An asthma-associated IL4R variant exacerbates airway inflammation by promoting conversion of regulatory T cells to TH17-like cells. Nature medicine, 22(9), 1013-1022.
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