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Cd 63 antibody

Manufactured by GeneTex
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Sourced in China

The CD-63 antibody is a laboratory reagent used to detect the presence of the CD63 cell surface protein in biological samples. CD63 is a member of the tetraspanin family and is commonly used as a marker for exosomes and other extracellular vesicles. The CD-63 antibody can be utilized in various immunoassay techniques to identify and quantify CD63-positive structures.

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Lab products found in correlation

P erk, by Cell Signaling Technology (1 mentions) Total erk, by Cell Signaling Technology (1 mentions) Kinase buffer, by Cell Signaling Technology (1 mentions) Total exosome rna and protein isolation kit, by Thermo Fisher Scientific (1 mentions) Flag tag (flag), by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) β tubulin, by Cell Signaling Technology (1 mentions) Sc 108080, by Santa Cruz Biotechnology (1 mentions) Claudin 5, by Cell Signaling Technology (1 mentions) U73122, by Bio-Techne (1 mentions) Pfgfr2, by Cell Signaling Technology (1 mentions) Neb 5 alpha competent e coli high efficiency, by New England Biolabs (1 mentions) Adenosine triphosphate (atp), by Cell Signaling Technology (1 mentions) U0126, by Cell Signaling Technology (1 mentions) Anti gfp, by Cell Signaling Technology (1 mentions) Metafectene pro, by Biontex (1 mentions) Multiskan go, by Thermo Fisher Scientific (1 mentions) Jsm it300lv, by JEOL (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Ab6728, by Abcam (1 mentions)

2 protocols using cd 63 antibody

1

Detailed Molecular Signaling Pathway Protocol

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FGF9 (catalogue # 273-F9-025) was purchased from R&D Systems. METAFECTENE® PRO (#T040) was purchased from Biontex. TRPA1 antibodies were purchased from Alomone (#ACC-037) and Santa-Cruz (#sc-166469). Total FGFR2 antibody (#sc-122) was purchased from Santa-Cruz. pFGFR2 (#3471), pERK (#4370), Total ERK (#9102) β-actin (#4970), Flag (#8146), Ki-67 (#9449), β-tubulin (#2146), Zona Occludin-1 (#5406), Claudin-5 (#4933), IgG antibody (#2729), anti-GFP (#2956) antibodies along with U0126 (#9903) were purchased from Cell Signalling. U73122 (#1268) was purchased from TOCRIS. NEB 5-alpha competent E.Coli (high efficiency) (#C2987H) cells purchased from New England Biolabs, were used for transformation. TRPA1 inhibitor HC030031 (#2896) was purchased from Tocris bioscience. ATP (#9804) and kinase buffer (#9802) were purchased from Cell Signalling. Occludin (H-279) (sc-5562) antibody, validated shRNAs18 (link), 55 (link) (sc-29218-V, sc-108080, and sc-29452-V), and DMA (sc-202459) were purchased from Santa Cruz Biotechnology, Inc. Strep antibody (# ab76949) was bought from abcam. CD-63 antibody (#GTX41877) from GeneTex was utilized as an exosomal marker (exosomal proteins were extracted using the Total Exosome RNA and Protein Isolation kit (#4478545, Invitrogen). All antibodies were used in 1:1000 dilutions for western blots.
Berrout J., Kyriakopoulou E., Moparthi L., Hogea A.S., Berrout L., Ivan C., Lorger M., Boyle J., Peers C., Muench S., Gomez J.E., Hu X., Hurst C., Hall T., Umamaheswaran S., Wesley L., Gagea M., Shires M., Manfield I., Knowles M.A., Davies S., Suhling K., Gonzalez Y.T., Carragher N., Macleod K., Abbott N.J., Calin G.A., Gamper N., Zygmunt P.M, & Timsah Z. (2017). TRPA1–FGFR2 binding event is a regulatory oncogenic driver modulated by miRNA-142-3p. Nature Communications, 8, 947.
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2

Characterization of Extracellular Vesicles

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The proteins of EVs were detected using a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, Massachusetts, America) according to the manufacturer's instructions. The optical density (OD) of proteins was measured at 562 nm by a microplate reader (Multiskan GO, Thermo Fisher Scientific). Marker proteins and vascular endothelial growth factor (VEGF) in EVs were detected using western blotting with the following antibodies: CD9 antibody (bs-2489 R, Bioss, Beijing, China), CD81 antibody (GTX31381, GeneTex, Southern California, America), VEGF antibody (AF5131, Affinity, Jiangsu, China), CD63 antibody (GTX41877, GeneTex) and β-actin (1:50,000; cat. No. AC026; ABclonal, China). The secondary antibody was a rabbit polyclonal secondary antibody to mouse IgG-H&L (ab6728, Abcam, Shanghai, China). The morphology of EVs was observed using a transmission electron microscope (JSM-IT300LV, JEOL, Beijing, China) with an EVs electron microscope analysis kit (Weihui Biology, Beijing, China). Finally, the diameter, distribution and number of EVs were analyzed by nanoparticle tracking analysis with a Nanosight NS300 (Beijing, China).
Yinhua Zhao M.M., Yanyu Shi M.M, & Lin H. (2023). Extracellular vesicles from hypoxia-pretreated adipose-derived stem cells regulate hypoxia/reoxygenation-induced human dermal microvascular endothelial apoptosis and autophagy in vitro. Heliyon, 9(2), e13315.
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