Log In Sign up
The largest database of trusted experimental protocols

Proteome profiler antibody array kits for human

Manufactured by R&D Systems
Visit Supplier

The Proteome Profiler Antibody Array Kits for human provide a platform for the simultaneous detection and relative quantification of multiple proteins. These array kits contain capture antibodies specific to various human proteins, allowing for the analysis of protein expression levels in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Gp130, by Cell Signaling Technology (2 mentions) P creb, by Cell Signaling Technology (2 mentions) Nf kb, by Cell Signaling Technology (2 mentions) Pnfkb, by Cell Signaling Technology (2 mentions) Pc jun, by Merck Group (2 mentions) Patf 2, by Merck Group (2 mentions) P stat3, by Cell Signaling Technology (2 mentions) Stat3, by Cell Signaling Technology (2 mentions) Hif 1α, by Cell Signaling Technology (2 mentions) Lamin b1, by Abcam (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

Proteome Profiler Antibody Array Kits for human angiogenesis factors Human Proteome Profiler Array kit Proteome Profiler™ Antibody Array kit Proteome Profiler Array Kit Proteome Profiler kit Proteome Profiler Array Proteome Profiler Human kits

2 protocols using proteome profiler antibody array kits for human

1

Reverse Western Blot Analysis of Angiogenic Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
For reverse western blot, Proteome Profiler Antibody Array Kits for human angiogenesis factors, chemokines, cytokines and phospho-kinases (R&D systems) were used, according to the manufacturer’s instructions. For western blot, 400,000 MDA-MB-231 or LECs (per well) were starved for 24 h, after which they were treated with Stattic (5–10 µM), S3I-201 (2.5–10 µM), or SP600125 (40 µM) and incubated for 60 min. After that, inducers, including TCM (30%), EGM, IL6-dep-TCM, IL6 or EGF were added. We followed the standard protocol for the rest of the procedure as described previously11 (link) applying antibodies of interest, including pStat3, HIF-1α, gp130, pNFkB, NFkB, IkBα, Stat3, pCREB, GAPDH (all from Cell Signaling), pc-Jun, pATF-2 (Sigma), CCR5, and Lamin B1 (Abcam). All the original gel images of immunoblot analyses are presented in Supplementary Fig. 25.
Lee E., Fertig E.J., Jin K., Sukumar S., Pandey N.B, & Popel A.S. (2014). Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. Nature communications, 5, 4715.
+ Open protocol
+ Expand
2

Reverse Western Blot Analysis of Angiogenic Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
For reverse western blot, Proteome Profiler Antibody Array Kits for human angiogenesis factors, chemokines, cytokines and phospho-kinases (R&D systems) were used, according to the manufacturer’s instructions. For western blot, 400,000 MDA-MB-231 or LECs (per well) were starved for 24 h, after which they were treated with Stattic (5–10 µM), S3I-201 (2.5–10 µM), or SP600125 (40 µM) and incubated for 60 min. After that, inducers, including TCM (30%), EGM, IL6-dep-TCM, IL6 or EGF were added. We followed the standard protocol for the rest of the procedure as described previously11 (link) applying antibodies of interest, including pStat3, HIF-1α, gp130, pNFkB, NFkB, IkBα, Stat3, pCREB, GAPDH (all from Cell Signaling), pc-Jun, pATF-2 (Sigma), CCR5, and Lamin B1 (Abcam). All the original gel images of immunoblot analyses are presented in Supplementary Fig. 25.
Lee E., Fertig E.J., Jin K., Sukumar S., Pandey N.B, & Popel A.S. (2014). Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. Nature communications, 5, 4715.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!