Human hepatocarcinoma cell lines (HuH-7, HepG2, SNU-449, SNU-387) were purchased from the Shanghai Institute for Biological Science (Shanghai, China). HepG2 and HuH-7 cells were cultured in Dulbecco's Minimal Essential Medium (DMEM; Gibco; Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco), 100 U/ml penicillin, and 100 mg/ml streptomycin. SNU-449 and SNU-387 cells were cultured in RPMI 1640 Medium (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin. Cells were maintained at 37°C in 5% CO2 and 95% air.
Snu 387
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SNU-387 is a laboratory centrifuge designed for general-purpose applications. It features a compact, benchtop design and can accommodate a variety of rotor types to accommodate different sample volumes and tube sizes. The centrifuge operates at adjustable speeds up to a maximum of 6,000 rpm to facilitate efficient separation of samples.
Automatically generated - may contain errors
Lab products found in correlation
Snu449, by Thermo Fisher Scientific (13 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (12 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (11 mentions)
Snu 387, by American Type Culture Collection (10 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (10 mentions)
Hep3b, by Thermo Fisher Scientific (10 mentions)
Snu 449, by American Type Culture Collection (7 mentions)
Hepg2, by Thermo Fisher Scientific (7 mentions)
Hep3b, by American Type Culture Collection (6 mentions)
Rpmi 1640, by Thermo Fisher Scientific (6 mentions)
Hepg2, by American Type Culture Collection (4 mentions)
Snu387, by National Collection of Authenticated Cell Cultures (4 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Mhcc97h, by Thermo Fisher Scientific (3 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (3 mentions)
Thle 2, by Thermo Fisher Scientific (3 mentions)
Dmem medium, by Thermo Fisher Scientific (3 mentions)
Snu 182, by Thermo Fisher Scientific (3 mentions)
Hep3b, by National Collection of Authenticated Cell Cultures (3 mentions)
Smmc 7721, by Thermo Fisher Scientific (2 mentions)
23 protocols using snu 387
1
Culturing Human Liver Cancer Cell Lines
2
Demethylation Assay for HCC Cells
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Human HCC cell lines (Bel-7402, HepG2, Huh7, MHCC-97H, MHCC-97L, SMMC-7721, SNU-387, and SNU-449) and immortalized normal liver cell line LO2 were purchased from American Type Culture Collection and Cell Resource Center of Shanghai Institutes. HepG2, Huh7, MHCC-97H, MHCC-97L, and LO2 were cultured in DMEM medium (Gibco) supplemented with 10% FBS (BI). Bel-7402, SMMC-7721, SNU-387, and SNU-449 were cultured in RPMI1640 medium (Gibco) supplemented with 10% FBS (BI). For demethylation treatment, HCC cells were treated with 10μM decitabine (Selleck, S1200) for 3 days and harvested for the following experiments.
3
Culturing Human Liver Cancer Cell Lines
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Different types of the human HCC cell lines (Huh-7, well-differentiated, epithelial phenotypic and mutation in p53; LM-3, high-metastatic, epithelial phenotypic and mutation in p53; SNU-387, poor-differentiated, mesenchymal phenotypic and mutation in p53; SNU-449, poor-differentiated, mesenchymal phenotypic and mutation in p53) were purchased from Shanghai Institute for Biological Science (Shanghai, China). Huh-7 and LM-3 cells were cultured in Dulbecco’s minimal essential medium (DMEM; Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin. SNU-387 and SNU-449 cells were cultured in RPMI 1640 Medium (1640; Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin. For normoxia, cells were maintained at 37 °C in 5% CO2 and 95% air; for hypoxia, cells were maintained at atmosphere containing 0.2% O2 and 5% CO2 in a hypoxia incubator.
4
Culturing Human Hepatocellular Carcinoma Cell Lines
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Human HCC cell lines HEP3B and SNU387 were purchased from ATCC. Human HCC cell lines Huh7 and MHCC-97H were purchased from the National Collection of Authenticated Cell Cultures (https://www.cellbank.org.cn/ ). HEP3B cells were cultured in Minimum Essential Media (MEM, Gibco, 11095080) Supplementaryemented with 1% Non-Essential Amino Acids (NEAA, Sigma Aldrich, M7145) and 10% fetal bovine serum (FBS, Gibco, 12662029). Huh7, MHCC-97H, and SNU387 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, 11995065) with 10% fetal bovine serum (FBS, Gibco, 12662029). All cells were cultured in a humidified incubator containing 5% CO2 at 37 °C.
5
Hepatocellular Carcinoma Cell Lines Cultivation
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The human HCC cell lines Hep3B, HepG2, SNU387, and SNU449 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultivated according to ATCC's instructions. Huh7 cells were purchased from the Chinese Academy of Science Cell Bank (Shanghai, China). Hep3B, HepG2, and Huh7 cells were cultured in Eagle's minimum essential medium (EMEM, Gibco, Grand Island, NY, USA); the SNU387 and SNU449 cells were cultured in RPMI 1640 medium (Gibco, Grand Island, NY, USA). All mediums were supplemented with 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA); all cells were maintained at 37°C in a humidified incubator with 5% CO2. Cisplatin was purchased from Selleck (Houston, TX, USA).
6
Cell Line Source and Culture Conditions
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THLE-2, MIHA, Hep3B and Huh7 cells were purchased from the National Collection of Authenticated Cell Cultures (https://www.cellbank.org.cn/ ). SNU-449, SNU-182, and SNU-387 were purchased from ATCC: The Global Bioresource Center (ATCC, https://www.atcc.org/ ). PLC/PRF/5 and MHCC-97H were provided by Prof. Lu Guo-dong of the School of Public Health, Guangxi Medical University. Hep3B was cultured in MEM medium (Gibco, Shanghai, China) supplemented with 10% FBS (Gibco, Shanghai, China). THLE-2 was cultured in BEGM medium (Lonza, CC3170, Hong Kong). SNU-449, SNU-182, and SNU-387 were cultured in RMPI-1640 medium (Gibco, Shanghai, China) supplemented with 10% FBS (Gibco, Shanghai, China). MIHA, LM3, Huh7, PLC5, and MHCC97-H were cultured in DMEM medium (Gibco, Shanghai, China) supplemented with 10% FBS (Gibco, Shanghai, China).
7
Cell Culture Protocol for Hepatocellular Carcinoma
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The human hepatocellular carcinoma cell lines SNU449, SNU387 and Huh7 were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences, Shanghai Institute of Cell Biology, Chinese Academy of Sciences. SNU449 and SNU387 cells were cultured in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.). Huh7 cells were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.). All culture media were supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 mg/ml streptomycin, and all cells were maintained at 37°C in a humidified incubator with 5% CO2.
8
Cell Culture of Hepatocellular Carcinoma Lines
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Two HCC cell lines, including SNU-387 and Huh7, and immortalized human hepatocytes (THLE-2) were purchased from YaJi Biological (Shanghai, China). SNU-387 cells were cultured in Roswell Park Memorial Institute (RPMI-1640; Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS). Huh7 cells were kept in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% FBS.
THLE-2 cells were maintained in Bronchial Epithelial Cell Growth Medium (BEGM; Gibco) containing 10% FBS. All cells were maintained in a thermostatic incubator at a constant temperature of 37°C with moist air and 5% CO2.
THLE-2 cells were maintained in Bronchial Epithelial Cell Growth Medium (BEGM; Gibco) containing 10% FBS. All cells were maintained in a thermostatic incubator at a constant temperature of 37°C with moist air and 5% CO2.
9
HCC Cell Line Characterization and miR-9 Analysis
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Human HCC cell lines (Hep3B, Huh7, SNU387 and SNU449) were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). Huh7 cells (epithelial phenotype HCC) were maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1% penicillin/streptomycin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). SNU387 and SNU449 cells (mesenchymal phenotype HCC) were cultured in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS and 1% penicillin/streptomycin. Hep3B cells (epithelial phenotype HCC) were cultured in minimum essential medium (MEM; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were maintained under 5% CO2 at 37°C in a humidified incubator. Cetuximab and the miR-9 mimic and inhibitor were all purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, China). The eIF-5A-2 siRNA and negative control siRNA were designed by and purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Furthermore, TargetScan (www.targetscan.org ) was to predict the associated miRNAs for eIF-5A-2. Briefly, we opened TargetScan was opened and the human species chosen. The gene symbol eIF-5A-2 was searched to obtain the predicted associated miRNAs.
10
Tissue Acquisition and Cell Culture for HCC Study
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Specimens of tumor and adjacent normal tissues were obtained from 60 HCC patients undergoing surgery at Department of Hepatobiliary Surgery, Sun Yat-sen Memorial Hospital of Sun Yat-Sen University (Guangzhou, China). The patients did not undergo any radiotherapy or chemotherapy before surgical operation. The normal and tumor tissues of 60 HCC patients were frozen immediately in liquid nitrogen and then stored at −80°C for RNA extraction. Detailed HCC patient information is listed in Table S1 . The Ethics Committee of Sun Yat-sen Memorial Hospital approved this study. All patients provided written informed consent for all treatments performed and to have their data utilized for research purposes. The human HCC cell line SNU-387 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The SNU-387 cell line was cultured in Dulbecco's Modified Eagle Medium (Gibco BRL, Grand Island, NY, USA), supplemented with 10% fetal bovine serum (Gibco BRL) and incubated with 5% CO2 at 37°C.
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