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2 protocols using smoothened

1

Stemness and EMT Markers in Cancer

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CD24, CD44, CD133, Nanog, Oct4, Sox2, KLF4, c‐Myc, Gli1, Gli2, Patched1, Patched2, Smoothened, Bcl‐2, Cyclin D1, E‐cadherin, N‐cadherin, Snail, Slug and Nanog antibodies were obtained from Cell Signaling Technology (Danvers, MA). Shh protein and anti‐β‐actin antibody were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA). α‐Mangostin (98% pure) was obtained from the LKT (St. Paul, MN). Accutase was purchased from Innovative Cell Technologies, Inc (San Diego, CA). Matrigel was purchased from BD Bioscience (San Jose, CA). Crystal violet was purchased from Sigma‐Aldrich (St. Louis, MO). TRIZOL was purchased from Invitrogen (Grand Island, NY). Luciferase assay kit was purchased from Promega.
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2

Stem Cell and EMT Markers

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Antibodies against CD24, CD133, c-Myc, Nanog, Oct4, Gli1, Gli2, Patched-1, Patched-2, Smoothened, Bcl2, XIAP, Cyclin D2, E-Cadherin, N-cadherin, Slug, Snail, Zeb1 and β-actin were obtained from Cell Signaling Technology (Danvers, MA). All other chemicals were purchased from Fisher Scientific (Suwanee, GA) and Sigma-Aldrich (St. Louis, MO).
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