Borane ammonia complex

Manufactured by Merck Group

Sourced in United States, Sao Tome and Principe

Borane-ammonia complex is a stable, white, crystalline compound. It is commonly used as a reducing agent in organic synthesis. The compound consists of a borane (BH3) molecule coordinated to an ammonia (NH3) molecule, forming a stable complex.

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Lab products found in correlation

Sodium hydroxide beads, by Merck Group (11 mentions) Iodomethane, by Merck Group (10 mentions) Pngase f, by New England Biolabs (9 mentions) Hplc grade water, by Mallinckrodt (8 mentions) Hplc grade methanol, by Thermo Fisher Scientific (7 mentions) Formic acid, by Merck Group (7 mentions) Acetic acid, by Thermo Fisher Scientific (6 mentions) Isopropanol, by Thermo Fisher Scientific (6 mentions) Dimethyl sulfoxide (dmso), by Merck Group (6 mentions) Sodium hydroxide (naoh), by Mallinckrodt (5 mentions) Acetonitrile, by Thermo Fisher Scientific (4 mentions) Acetonitrile, by Avantor (4 mentions) G7 reaction buffer, by New England Biolabs (3 mentions) Glycoprotein denaturing buffer, by New England Biolabs (3 mentions) Ms grade formic acid, by Merck Group (3 mentions) Microspin columns, by Harvard Apparatus (3 mentions) Np 40, by New England Biolabs (2 mentions) Fetal bovine serum (fbs), by American Type Culture Collection (2 mentions) Mda mb 231, by American Type Culture Collection (2 mentions) Pooled human blood serum, by Merck Group (2 mentions)

19 protocols using borane ammonia complex

Breast Cancer Cell Line Cultivation

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Breast cancer cell line MDA-MB-231, MDA-MB-361, HTB131, HTB22 and CRL-1620 were purchased from American Type Culture Collection (ATCC). Breast cancer cell line MDA-MB-231BR was a generous gift from Dr. Paul Lockman (Texas Tech University Health Sciences Center, School of Pharmacy, Amarillo, TX). RPMI-1640 Medium, Eagle’s Minimum Essential Medium (EMEM), Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal Bovine Serum (FBS) and Penicillin-Streptomycin Solution (100X) were purchased from ATCC. Corning Trypsin EDTA 1 × (0.25% Trypsin/2.21 mM EDTA) was purchased from Fisher. Cell culturing plates and flasks were purchased from Corning. Mammalian total RNA Extraction Kit was purchased from Sigma-Aldrich. Borane-ammonia complex, dimethyl sulfoxide (DMSO), sodium hydroxide beads, iodomethane, and formic acid (FA) were obtained from Sigma-Aldrich (St. Louis, MO). PNGase F and digestion buffers, including 10 × G7 reaction buffer (0.5 M sodium phosphate), RXN buffer, 10× glycoprotein denaturing buffer (5% SDS, 0.4 M DTT), and NP-40 were acquired from New England Biolabs (Ipswich, MA). HPLC grade methanol, isopropanol, and acetic acid were procured from Fisher Scientific (Pittsburgh, PA). Acetonitrile was obtained from JT Baker (Phillipsburg, NJ). HPLC grade water and sodium hydroxide were purchased from Mallinckrodt Chemicals (Phillipsburg, NJ).

Peng W., zhu R., Zhou S., Mirzaei P, & Mechref Y. (2019). Integrated Transcriptomics, Proteomics, and Glycomics Reveals the Association between Up-regulation of Sialylated N-glycans/Integrin and Breast Cancer Brain Metastasis. Scientific Reports, 9, 17361.

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Synthesis and Characterization of Perovskite Catalysts

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LaCl₃·7H₂O (Alfa
Aesar), SrCl₂·6H₂O (Alfa Aesar), SrCO₃ (Alfa Aesar), CaCO₃ (Alfa Aesar), BaCO₃ (Alfa Aesar), Co₃O₄ (Alfa Aesar), La₂O₃ (Alfa Aesar), CaCl₂·2H₂O
(EM Science), RuCl₃·xH₂O (Sigma-Aldrich), borane ammonia complex (Sigma-Aldrich), Pt/C (E-TEK
Inc.), and KOH (VWR) were used as received without further purification.
Nanopure water (18 MΩ) was obtained from a Barnstead Nanopure
Analytical Ultrapure water system.

Badding C.K., Soucy T.L., Mondschein J.S, & Schaak R.E. (2018). Metal Ruthenate Perovskites as Heterogeneous Catalysts for the Hydrolysis of Ammonia Borane. ACS Omega, 3(3), 3501-3506.

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Glycoprotein Analysis by HPLC

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HPLC grade solvents, including water, acetonitrile, methanol and isopropanol alcohol, were purchased from Fisher Scientific (Pittsburgh, PA). Sodium hydroxide beads (20–40 mesh, 97%), iodomethane, acetic acid, formic acid, dimethyl sulfoxide (DMSO) and borane-ammonia complex were obtained from Sigma-Aldrich (St. Louis, MO). Empty spin columns were purchased from Harvard Apparatus (Holliston, MA). PNGase F and digestion buffer were from New England Biolabs (Ipswich, MA). The glycoprotein samples, including ribonuclease B, fetuin, α acidic glycoprotein and pooled human blood serum, were bought from Sigma-Aldrich (St. Louis, MO). The standard glycan samples were purchased from Chemily Glycoscience (Atlanta, GA).

Zhou S., Huang Y., Dong X., Peng W., Veillon L., Kitagawa D.A., Aquino A.J, & Mechref Y. (2017). Isomeric Separation of Permethylated Glycans by Porous Graphitic Carbon (PGC)-LC-MS/MS at High-Temperatures. Analytical chemistry, 89(12), 6590-6597.

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Synthesis of Pt-Bi Nanoalloy Catalysts

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Chloroplatinic acid hexahydrate (H₂PtCl₆·6H₂O, 99.9%), bismuth nitrate (Bi(NO₃)₃·5H₂O, 99.0%), ethylene glycol (EG, 99%), polyvinylpyrrolidone (PVP, K30, 99.9%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). The borane–ammonia complex (H₃N·BH₃, 90%), commercial Pt/C (Pt/C, 20 wt%), and Nafion 117 solution (5%) were purchased from Sigma Aldrich (St. Louis, MO, USA). Ultrapure water (18.2 MΩ cm) was used in the experiment.

Wu X., Wang D., Kang X., Zhang D., Yan Y., Guo G., Sun Z, & Wang X. (2022). Displacement Reaction-Assisted Synthesis of Sub-Nanometer Pt/Bi Boost Methanol-Tolerant Fuel Cells. Nanomaterials, 12(8), 1301.

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Serum Haptoglobin Analysis in Liver Diseases

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PNGase F was obtained from New England Biolabs (Ipswich, MA). The mouse anti-human haptoglobin antibody was purchased from Abcam (Cambridge, MA). Borane-ammonia complex, sodium hydroxide beads, and iodomethane were acquired from Sigma-Aldrich (St. Louis, MO). Dimethyl sulfoxide (DMSO) was bought from Mallinckrodt Chemicals (Phillipsburg, NJ). HPLC grade methanol and acetonitrile were purchased from Fisher Scientific (Fair Lawn, NJ). HPLC water was obtained from Avantor (Central Valley, PA). Spin columns were purchased from Harvard Apparatus (Holliston, MA).
Serum samples were provided by the University Hospital, Ann Arbor, Michigan according to IRB approval (10 cases of hepatocellular carcinoma (HCC) and 10 cases of liver cirrhosis.) The clinical information associated with the samples used in this study are summarized in Table 1 and Table S1.

Huang Y., Zhou S., Zhu J., Lubman D.M, & Mechref Y. (2017). LC-MS/MS Isomeric Profiling of Permethylated N-Glycans Derived from Serum Haptoglobin of Hepatocellular Carcinoma (HCC) and Cirrhotic Patients. Electrophoresis, 38(17), 2160-2167.

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Glycoprotein Profiling and Analysis

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Model glycoproteins, ribonuclease B (RNase B) from bovine pancreas, fetuin from bovine serum, porcine thyroglobulin (PTG), pooled human blood serum (HBS), borane-ammonia complex, sodium hydroxide beads, dimethyl sulfoxide (DMSO), iodomethane, trifluoro acetic acid and formic acid were purchased from Sigma-Aldrich (St. Louis, MO). Cell line MDA-MB-231 was purchased from American Type Culture Collection (ATCC, Manassas, VA), and MDA-MB-231BR was a generous gift from Dr. Paul Lockman (Texas Tech Health Sciences Centers, School of Pharmacy, Amarillo, TX). PNGase F, 10×G7 reaction buffer ((50 mM sodium phosphate buffer, pH 7.5), 10× glycoprotein denaturing buffer ((50 mM sodium phosphate buffer, pH 7.5, 5% SDS, 0.4 M dithiothreitol), and nonionic NP-40 detergent were acquired from New England Biolabs (Ipswich, MA). HPLC grade methanol, isopropanol, and acetic acid were procured from Fisher Scientific (Pittsburgh, PA). Acetonitrile (ACN) was obtained from JT Baker (Phillipsburg, NJ). HPLC grade water and sodium hydroxide were purchased from Mallinckrodt Chemicals (Phillipsburg, NJ).

Zhou S., Hu Y., DeSantos-Garcia J.L, & Mechref Y. (2015). Quantitation of Permethylated N-Glycans through Multiple-Reaction Monitoring (MRM) LC-MS/MS. Journal of the American Society for Mass Spectrometry, 26(4), 596-603.

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Comprehensive Workflow for Glycoprotein Analysis

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HPLC grade methanol, isopropanol, and acetic acid were procured from Fisher Scientific (Pittsburgh, PA). Acetonitrile (ACN) and acetone were acquired from JT Baker (Phillipsburg, NJ). HPLC grade water and sodium hydroxide were purchased from Mallinckrodt Chemicals (Phillipsburg, NJ). Borane-ammonia complex, sodium hydroxide beads, dimethyl sulfoxide (DMSO), iodomethane, trifluoroacetic acid (TFA), and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO). PNGase F with 10×G7 reaction buffer (0.5 M sodium phosphate) was obtained from New England Biolabs (Ipswich, MA). AminoxyTMT^™ reagent was obtained from Thermo Fisher Scientific (Rockford, IL).
RNase B and fetuin from bovine serum were purchased from Sigma-Aldrich (St. Louis, MO). Human blood serum from disease free and patients with Barrett’s disease (BE), high-grade dysplasia (HGD) and esophageal adenocarcinoma (EAC) was provided by Dr. Zane Hammoud (Henry Ford Hospital, Detroit, MA). All cell lines were purchased from American Type Culture Collection (ATCC; Manassas, VA) except for the subline MDA-MB-231Br, which was provided by Dr. Paul Lockman and Dr. Quentin Smith (TTU Pharmacy School, Amarillo, TX).

Zhou S., Hu Y., Veillon L., Snovida S.I., Rogers J.C., Saba J, & Mechref Y. (2016). Quantitative LC-MS/MS Glycomic Analysis of Biological Samples Using AminoxyTMT™. Analytical chemistry, 88(15), 7515-7522.

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Borane-Ammonia Stabilizes Triethanolamine Color

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Example 1

Color Stability of a Color-Reduced Triethanolamine Composition

Triethanolamine (1000 g, Sigma Aldrich, 99% purity) having a Pt—Co Color Value of 23 is mixed with a borane-ammonia complex (100 mg, obtained from Sigma Aldrich, 95% purity). The mixture is vigorously shaken to allow the borane-ammonia complex to distribute homogenously in the Triethanolamine Over a period of 6 hours the mixture clears. A sample is taken for color analysis, and found to have a Pt—Co Color Value that was calculated to be less than 15 after 2 years at room temperature (20-25° C.).

US10662144B2. Method of reducing color in alkanolamine compositions and compositions produced thereby (2020-05-26). SABIC GLOBAL TECHNOLOGIES B.V. [NL]. Inventors: Feng Xu [SA], Flaiyh Al-Anazi [SA].

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Glycoprotein N-glycan Analysis Protocol

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Borane-ammonia complex, sodium hydroxide beads, dimethyl sulfoxide (DMSO), iodomethane, trifluoro Acetic acid, chloroform, ammonium bicarbonate, and MS-grade formic acid were obtained from Sigma-Aldrich (St. Louis, MO). Micro-spin columns were supplied by Harvard Apparatus (Holliston, MA). PNGase F (500,000 units/mL) was purchased from New England Biolabs Inc. (Ipswich, MA). Acetic acid and acetonitrile were purchased from Fisher Scientific (Pittsburgh, PA), and HPLC-grade water was acquired from Mallinckrodt Chemicals (Phillipsburg, NJ).

Wildburger N.C., Zhou S., Zacharias L.G., Kroes R.A., Moskal J.R., Schmidt M., Mirzaei P., Gumin J., Lang F.F., Mechref Y, & Nilsson C.L. (2015). Integrated Transcriptomic and Glycomic Profiling of Glioma Stem Cell Xenografts. Journal of proteome research, 14(9), 3932-3939.

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Glycoprotein Characterization by HPLC

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HPLC grade methanol, isopropanol, and acetic acid were procured from Fisher Scientific (Pittsburgh, PA). Acetonitrile was acquired from JT Baker (Phillipsburg, NJ). HPLC grade water and sodium hydroxide were purchased from Mallinckrodt Chemicals (Phillipsburg, NJ). Borane-ammonia complex, sodium hydroxide beads, dimethyl sulfoxide (DMSO), iodomethane, trifluoroacetic acid (TFA) and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO). PNGase F with 10×G7 reaction buffer (0.5 M sodium phosphate), 10× glycoprotein denaturing buffer (5% SDS, 0.4 M DTT), and NP-40 were obtained from New England Biolabs (Ipswich, MA).

Zhou S., Hu Y, & Mechref Y. (2016). High-Temperature LC-MS/MS of Permethylated Glycans Derived from Glycoproteins. Electrophoresis, 37(11), 1506-1513.

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