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Abi 3730 l genetic analyzer

Manufactured by Thermo Fisher Scientific
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The ABI 3730xl Genetic Analyzer is a high-throughput DNA sequencing instrument designed for automated DNA fragment analysis. It utilizes capillary electrophoresis technology to separate and detect fluorescently labeled DNA fragments. The instrument is capable of processing multiple samples simultaneously and generating high-quality DNA sequence data.

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Lab products found in correlation

Bigdye terminator chemistry, by Thermo Fisher Scientific (2 mentions) Taq dna polymerase, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

ABI 3730 (234 citations) ABI 3730 Genetic Analyzer (214 citations) 3730 Genetic Analyzer (85 citations) 3130×l Genetic Analyzer (29 citations) ABI 3730 analyzer (12 citations) ABI 3730×l (3 citations) ABI PRISM 3730×L GENETIC ANALYZER (3 citations)

2 protocols using abi 3730 l genetic analyzer

1

Sanger Sequencing of PIK3R2 Mutations

Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed confirmation of constitutional mutations by direct
Sanger sequencing. PCR amplification was performed with 50 ng of genomic DNA
using Taq DNA polymerase (Applied Biosystems). Primers used to amplify the
coding and flanking noncoding regions of PIK3R2 were
designed using Primer 3. Double-stranded DNA sequence analysis was performed
using the Big Dye Terminator chemistry (Applied Biosystems), and reactions
were run on the ABI 3730_l Genetic Analyzer (Applied Biosystems). Sequence
chromatograms were analyzed using Mutation Surveyor software version 3.30.
Sequences were compared with normal control samples and the reference
sequences for PIK3R2.
Mirzaa G., Conti V., Timms A.E., Smyser C.D., Ahmed S., Carter M., Barnett S., Hufnagel R.B., Goldstein A., Narumi-Kishimoto Y., Olds C., Collins S., Johnston K., Deleuze J.F., Nitschké P., Friend K., Harris C., Goetsch A., Martin B., Boyle E.A., Parrini E., Mei D., Tattini L., Slavotinek A., Blair E., Barnett C., Shendure J., Chelly J., Dobyns W.B, & Guerrini R. (2015). Characterization of mutations of the phosphoinositide-3-kinase regulatory subunit, PIK3R2, in perisylvian polymicrogyria: a next generation sequencing study. The Lancet. Neurology, 14(12), 1182-1195.
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2

Sanger Sequencing of PIK3R2 Mutations

Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed confirmation of constitutional mutations by direct
Sanger sequencing. PCR amplification was performed with 50 ng of genomic DNA
using Taq DNA polymerase (Applied Biosystems). Primers used to amplify the
coding and flanking noncoding regions of PIK3R2 were
designed using Primer 3. Double-stranded DNA sequence analysis was performed
using the Big Dye Terminator chemistry (Applied Biosystems), and reactions
were run on the ABI 3730_l Genetic Analyzer (Applied Biosystems). Sequence
chromatograms were analyzed using Mutation Surveyor software version 3.30.
Sequences were compared with normal control samples and the reference
sequences for PIK3R2.
Mirzaa G., Conti V., Timms A.E., Smyser C.D., Ahmed S., Carter M., Barnett S., Hufnagel R.B., Goldstein A., Narumi-Kishimoto Y., Olds C., Collins S., Johnston K., Deleuze J.F., Nitschké P., Friend K., Harris C., Goetsch A., Martin B., Boyle E.A., Parrini E., Mei D., Tattini L., Slavotinek A., Blair E., Barnett C., Shendure J., Chelly J., Dobyns W.B, & Guerrini R. (2015). Characterization of mutations of the phosphoinositide-3-kinase regulatory subunit, PIK3R2, in perisylvian polymicrogyria: a next generation sequencing study. The Lancet. Neurology, 14(12), 1182-1195.
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