The fixation of PDLSCs was performed with 4% paraformaldehyde for 15–20 min, and then incubated with 0.2% Triton X-100 (Solarbio) for 10 min for permeabilization. PDLSCs were blocked with 10% goat serum for 1 h at room temperature, and then incubated with anti-SOD2 primary antibody (1:200, 24127-1-AP, Proteintech), overnight at 4°C, and then incubated with CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) (SA00013-4, Proteintech) and protected from light for 1 h at room temperature. DAPI (Solarbio) staining for the nuclei in PDLSCs was carried out. PDLSCs were visualized using the fluorescence microscope (OLYMPUS, Tokyo, Japan).
Coralite594 conjugated goat anti rabbit igg h l sa00013 4
Manufactured by Proteintech
Sourced in United Kingdom
CoraLite594-conjugated Goat Anti-Rabbit IgG (H+L) (SA00013-4) is a secondary antibody conjugated to the fluorescent dye CoraLite594. It is designed to detect and visualize rabbit primary antibodies in various immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using coralite594 conjugated goat anti rabbit igg h l sa00013 4
1
Immunofluorescence Staining of PDLSCs
2
Quantifying DNA Damage Response in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded on glass coverslips and cultured in the respective media containing PARP inhibitors for 4 days. Then, the cells were fixed in 4% paraformaldehyde for 20 min and permeabilized with Triton X-100 for 10 min at room temperature. After being blocked, the cells were incubated with the primary antibody against γ-H2AX (1:800, ab81299, Abcam, Cambridge, UK) at 4°C overnight, followed by incubation with CoraLite594-conjugated goat anti-rabbit IgG (H+L) (SA00013-4, ProteinTech) for 2 h at room temperature. The nuclei were stained using 4′,6-diamidino-2-phenylindole dihydrochloride for 5 min.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!