P. fucata meat proteins and their hydrolysates were hydrolyzed using 6 mol L−1 HCl solution at 110 °C for 22 h according to the method of Arise et al.18 (link) An L-8900 Automatic Amino Acid Analyzer (Hitachi High-Tech Corporation, Tokyo, Japan) was employed to determine the amino acid composition. The sample was analyzed for 18 amino acids. The concentrations of the different amino acids in the hydrolysate were expressed as g/100 g.
L 8900 automatic amino acid analyzer
Manufactured by Hitachi
Sourced in Japan
The L-8900 is an automatic amino acid analyzer manufactured by Hitachi. The core function of this equipment is to analyze and quantify the amino acid composition of various samples through ion exchange chromatography.
Automatically generated - may contain errors
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55 protocols using l 8900 automatic amino acid analyzer
1
Amino Acid Analysis of P. fucata Proteins
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P. fucata meat proteins and their hydrolysates were hydrolyzed using 6 mol L−1 HCl solution at 110 °C for 22 h according to the method of Arise et al.18 (link) An L-8900 Automatic Amino Acid Analyzer (Hitachi High-Tech Corporation, Tokyo, Japan) was employed to determine the amino acid composition. The sample was analyzed for 18 amino acids. The concentrations of the different amino acids in the hydrolysate were expressed as g/100 g.
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P. fucata meat proteins and their hydrolysates were hydrolyzed using 6 mol L−1 HCl solution at 110 °C for 22 h according to the method of Arise et al.18 (link) An L-8900 Automatic Amino Acid Analyzer (Hitachi High-Tech Corporation, Tokyo, Japan) was employed to determine the amino acid composition. The sample was analyzed for 18 amino acids. The concentrations of the different amino acids in the hydrolysate were expressed as g/100 g.
2
Amino Acid Profiling in Vegetables and Pulses
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A Hitachi L-8900 Automatic Amino Acid Analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan) with a 4.6 mm (ID)×60 mm ion exchange column (Hitachi High-Technologies Corporation) was used to determine the amino acid profiles of the vegetable and pulse samples. The following analyzer settings were used for the analysis: buffer flow rate of 0.4 mL/min, reagent flow rate of 0.35 mL/min, reactor heater temperature of 135°C, column temperature of 75°C, auto-sampler temperature of 5~8°C, run time of 35.3 min (sulfur-containing amino acids) or 56.3 min (all other amino acids), sample injection volume of 20 μL, and detection wavelength of 570 nm (proline) or 440 nm (all other amino acids). Protein hydrolysate buffer set (PH-SET KANTO, Kanto Chemical Co., Inc., Tokyo, Japan) and hydrochloric acid (Wako Pure Chemical Industries, Ltd.) were used as mobilephase solvents (10 (link),11 ). A standard amino acid mixture of cysteic acid and methionine sulfone (20 μL/mL) was diluted to 100 μmol/L for amino acid quantification and calibration. An external standard was used to calculate the concentration of each amino acid. Amino acid concentrations are reported in g/100 g.
3
Amino Acid Profiling Methodology
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Amino acid contents were measured using ninhydrin reagent and separated by cation-exchange chromatography, using an L-8900 automatic amino acid analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan) and a #2622PF column (60 × 4.6 mm) following the method of Jo et al. [25 (link)]. The sample (1 g) was hydrolyzed with 10 mL of HCl (6 mol/L) at 110 ± 1 °C for 22 h, cooled to 25 °C, and then filtered. The solvent was removed at 50 °C by rotary evaporator (Zhengzhou Kaixiang instrument equipment Co., Ltd., Zhengzhou, Henan, China). The sodium citrate buffer solution was added to the dried sample, and the mixture was shaken evenly, filtered through 0.22 μm membrane and transferred to the injection bottle as sample determination solution. Samples were measured under the following conditions: AccQ-Tag Ultra C18 column, detection wavelength 260 nm, column temperature 49 °C, sample temperature 20 °C, and flow rate 0.7 mL/min.
4
Quantification of Amino Acid Biomarkers
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To validate the metabolite results, potential metabolite markers including glycine, L‐glutamic acid, and L‐aspartic acid in serum and brain were quantified using an automatic amino acid analyzer. Briefly, 200 μl of serum and 400 μl of 8% s‐sulfosalicylic acid (8 g sulfosalicylic acid dissolved in 100 ml pure water) were mixed in a 1.5‐ml Eppendorf tube. For tissue samples, 400 μl of 10% s‐sulfosalicylic acid (10 g sulfosalicylic acid dissolved in 100 ml pure water) was added to 50 mg tissue in a 2‐ml Eppendorf tube and then grind with a high‐speed tissue grinder. After being centrifuged at 14,000 rpm for 15 min at 4 °C, the supernatant was filtered through a 0.45‐μm membrane. The expression levels of glycine, l ‐glutamic acid and l ‐aspartic acid were quantified by a Hitachi L‐8900 automatic amino acid analyzer (Hitachi High‐Technologies).
5
Amino Acid Profiling of Edible Tissues
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The FAAs were extracted according to the method of Liu et al. (2021) (link). Briefly, 5 g of an edible tissue was dissolved in 10 mL of 0.02 M dilute hydrochloric acid and homogenized for 30 s, afterward, the resulting solution was subjected to ultrasound for 5 min. Thereafter, the homogenate was centrifuged for 10 min at 5000 rpm and °C4°C, and the supernatant was maintained at °C4°C. The precipitate was treated again, following the aforementioned procedure, to obtain another supernatant, and the two obtained supernatants were combined. The processed samples were diluted to a final volume (25 mL) with Milli-Q purified water (Millipore Sigma). Subsequently, 2 mL of 5% sulfosalicylic acid was mixed with 2 mL of the diluent, and the mixed diluent was centrifuged again (1 × 104 rpm, °C4°C) for 10 min. Next, the supernatant was filtered through a 0.22-μm filter membrane-aquo system and analyzed with an L-8900 automatic amino acid analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan). All the samples were analyzed in triplicates.
6
Amino Acid Content Quantification
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The CSAE powder and CSOL were hydrolyzed with 6 mol/l HCl at 110±1°C for 22 h. Following vacuum drying, the samples were dissolved in 1 ml pH 2.2 buffer (19.6 g sodium citrate and 16.5 ml hydrochloric dissolved in 1 l of deionized water; pH 2.2). The amino acid content was quantified by an L-8900 automatic amino acid analyzer (Hitachi High-Technologies Corporation).
7
Amino Acid Composition and Protein Content Determination
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Amino acid composition was determined using an L-8900 automatic Amino Acid Analyzer (Hitachi High-Technologies Co., Japan) equipped with a 60×4.6 mm i.d., 1 μm Hitachi ion exchange resin column, according to the method reported by Liu et al. (2015) (link). Protein content was determined using automatic Kjeldahl apparatus (Kjeltec 8400, FOSS Co., Ltd, Denmark).
8
Amino Acid Hydrolysis and Analysis
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For hydrolytic amino acids, 0.1 g of each sample was placed in a 16 mm × 25 mm screw-cap tube and dissolved in 10 mL HCl (6 mol/L). The tubes were closed, placed in an electric oven at 110°C for 24 h, cooled, and then their content was diluted to 50 mL with ultrapure water. Then, 1mL of each diluted sample was removed and evaporated at 60°C until dry. The residue was then reconstituted with 2 mL of HCl (0.02 mol/L) and filtered through a 0.45-μm membrane. Subsequently, the contents of amino acids were analyzed using a Hitachi L-8900 Automatic Amino Acid analyzer (Hitachi Ltd.) fitted with a 2622SC-PH sodium citrate equilibrium ion exchange column (Hitachi Ltd.) and post column derivatization with ninhydrin, according to the manufacture’s standard procedure [32 ].
9
Amino Acid Analysis Protocol
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Each sample (0.1 g) was placed in a 2-mL tube and dissolved in HCl (0.02 mol/L, 1 mL). After closing the tubes and mixing for 15 min at room temperature, the tubes were centrifuged at 12,000 × g for 15 min and the supernatant was collected. The supernatant (0.8mL) was mixed with 10% sulfosalicylic acid (0.8 mL) by vortexing for 2 min, and then after centrifuging at 12,000 × g for 15 min, the supernatant was collected. Subsequently, the supernatants were filtered through a 0.22-μm membrane for testing. Eventually, samples were analyzed by chromatography using a Hitachi L-8900 Automatic Amino Acid analyzer (Hitachi Ltd., Tokyo, Japan) fitted with a 2622SC-PF lithium citrate equilibrated ion exchange column (Hitachi Ltd.) and post column derivatization with ninhydrin, according to the manufacturer’s standard procedure [32 ].
10
Quantitative Analysis of Free Amino Acids
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For plasma, 0.5 ml sample was collected and 2.5 ml 4% sulfosalicylic acid was added. Then, the mixture was centrifuged at 14,000 rpm for 30 min at 4°C. The resultant supernatant was carefully collected for the determination of FAA content. For liver and muscle, the samples were dried to a constant weight at 105°C. 0.5 g dry sample was weighed and ground using a mortar. 5 ml 4% sulfosalicylic acid was added and the mixture was centrifuged at 14,000 rpm for 30 min at 4°C. The resultant supernatant was carefully collected for the determination of FAA content. For the determination of FAA, the collected supernatant was passed through two Millipore micro filters (0.22 μm pore size) before assaying FAAs level. FAA contents was determined by using a Hitachi L-8900 automatic amino acid analyzer (Hitachi, Japan), equipped with an ion exchange resin column (4.6 mm × 60 mm).
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