Ac 33f1

Manufactured by Immunodiagnostic Systems

Sourced in United Kingdom

The AC-33F1 is a fully automated immunoassay analyzer designed for the analysis of a wide range of analytes. It features a high-throughput capacity and is capable of performing various immunoassay techniques. The AC-33F1 is a compact and efficient laboratory equipment solution.

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Lab products found in correlation

Ac 06f1, by Immunodiagnostic Systems (8 mentions) Sb tr103, by Immunodiagnostic Systems (4 mentions) Ac 12f1, by Immunodiagnostic Systems (2 mentions) Kge009, by R&D Systems (1 mentions) Milliplex map kit, by Merck Group (1 mentions) Luminex 200 analyzer, by DiaSorin (1 mentions) Ab108821, by Abcam (1 mentions) Mouse trap, by Immunodiagnostic Systems (1 mentions) Synergy ht, by Agilent Technologies (1 mentions) C2 plus, by Nikon (1 mentions) Sb tr102, by Immunodiagnostic Systems (1 mentions) Mbs2533600, by MyBioSource (1 mentions)

14 protocols using ac 33f1

Quantifying Serum Biomarkers in Mice

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Serum was collected from mice by cardiac puncture under terminal anaesthetic. Briefly, whole blood was left at room temperature for 30 min prior to centrifugation for 20 min at 12,000 rpm. Serum was aspirated and stored at −80 °C prior to analysis. Serum-free (non-corticosteroid binding globulin bound) corticosterone levels were measured using a commercially available sandwich ELISA designed to specifically detect active (but not inactive 11-DHC) steroid (cat no: KGE009, R&D systems, Abingdon, UK). Serum was analysed in accordance with the manufacturer’s instructions and data expressed as ng/mL. Serum P1NP was determined using a commercially available sandwich ELISA (cat no: AC-33F1, Immunodiagnosticsystems, Tyne & Wear, UK) in accordance with the manufacturer’s instructions and data expressed as ng/mL. Serum TRACP 5b was determined using a commercially available sandwich ELISA (cat no: SB-TR103, Immunodiagnosticsystems, Tyne & Wear, UK) in accordance with the manufacturer’s instructions and data expressed as U/µL.

Fenton C.G., Crastin A., Martin C.S., Suresh S., Montagna I., Hussain B., Naylor A.J., Jones S.W., Hansen M.S., Gorvin C.M., Price M., Filer A., Cooper M.S., Lavery G.G., Raza K, & Hardy R.S. (2022). 11β-Hydroxysteroid Dehydrogenase Type 1 within Osteoclasts Mediates the Bone Protective Properties of Therapeutic Corticosteroids in Chronic Inflammation. International Journal of Molecular Sciences, 23(13), 7334.

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Bone Turnover Biomarkers in Fasting Plasma

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Fasting plasma was collected at euthanasia via cardiac puncture to measure concentrations of C-terminal telopeptide (CTX) for osteoclast activity (catalog #AC-06F1, Immunodiagnostic Systems, Tyne and Wear, United Kingdom) and Procollagen 1 Intact N-Terminal Propeptide (P1NP) for osteoblast activity (catalog #AC-33F1, Immunodiagnostic Systems, Tyne and Wear, United Kingdom). Plasma was analyzed at the Maine Medical Center Research Institute Physiology Core. One outlier was removed from the CTX measurement (Low Glycemic) after a Grubbs' test.

Castaneda M., Smith K.M., Nixon J.C., Hernandez C.J, & Rowan S. (2021). Alterations to the gut microbiome impair bone tissue strength in aged mice. Bone Reports, 14, 101065.

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Bone Turnover Biomarkers Evaluation

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All quantifications were made at the Department of Clinical Pathology of São João Hospital Centre EPE, Porto, Portugal. Circulating concentrations of N-terminal propeptide of type I procollagen (PINP; AC-33F1 from Immunodiagnostic Systems Limited, Tyne & Wear, United Kingdom), osteopontin (OPN; JP27360 from Immuno-Biological Laboratories, Co. Limited, Gunma, Japan), osteocalcin (OTN; AC-12F1 from Immunodiagnostic Systems Limited, Tyne & Wear, United Kingdom), and degradation products from C-terminal telopeptides of type I collagen (RatLapsTM, CTX-1; AC-06F1 from Immunodiagnostic Systems Limited, Tyne & Wear, United Kingdom) were evaluated by using enzyme immunoassay/enzyme-linked immunosorbent assay (EIA/ELISA), according to the instructions provided by the suppliers. Circulating concentrations of receptor activator of nuclear factor-kappaB ligand (RANKL; RRNKLMAG-31K-01, Single Plex) and osteoprotegerin (OPG; RBN1MAG-31K, two plex) were determined by a plex bead assay performed according to protocols (MILLIPLEX^® MAP kits) of Millipore Corporation (Billerica, MA, USA). Quantifications were performed using a Luminex 200 analyzer (Luminex Corporation, Austin, TX, USA).

Pereira C., Guede D., Durães C., Brandão I., Silva N., Passos E., Bernardes M., Monteiro R, & Martins M.J. (2019). Differential Modulation of Cancellous and Cortical Distal Femur by Fructose and Natural Mineral-Rich Water Consumption in Ovariectomized Female Sprague Dawley Rats. Nutrients, 11(10), 2316.

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Serum Biomarkers of Bone Metabolism

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Serum samples were obtained by cardiac puncture at sacrifice followed by centrifugation in serum separator tubes (BD Microtainer #365967, Franklin Lakes, NJ) and stored at −80°C until analysis. A mouse TRAcP-5b ELISA (MouseTrap™; #SB-TR103, Immunodiagnostic Systems, Gaithersburg, MD) was used to measure the circulating bone resorption marker tartrate-resistant acid phosphatase 5b. Additional aliquots of serum were analyzed in a rat/mouse procollagen I intact N-terminal (P1NP) enzyme immunoassay (#AC-33F1, Immunodiagnostic Systems, Gaithersburg, MD) for quantification of bone formation markers. Serum glucocorticoids were measured using a corticosterone ELISA (#ab108821, Abcam, Cambridge, MA) using a 100-fold dilution of mouse serum and provided protocols. All ELISA plates were measured with a microplate reader (BioTek Synergy HT, Winooski, VT) using Gen5 software and readings at 405 (P1NP) and 450 nm (TRAcP-5b, Corticosterone) wavelengths, as recommended for each assay. Serum data were normalized to calibrants and standards provided in respective ELISA kits.

Pierce J.L., Ding K.H., Xu J., Sharma A.K., Yu K., del Mazo Arbona N., Rodríguez-Santos Z., Bernard P.J., Bollag W.B., Johnson M.H., Hamrick M.W., Begun D.L., Shi X.M., Isales C.M, & McGee-Lawrence M.E. (2019). The glucocorticoid receptor in osteoprogenitors regulates bone mass and marrow fat. The Journal of endocrinology.

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Serum Biomarker Quantification in Mice

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Serum was collected from mice by cardiac puncture under terminal anaesthetic. Briefly, whole blood was left at room temperature for 30 min prior to centrifugation for 20 min at 12,000 rpm. Serum was aspirated and stored at − 80 °C prior to analysis. Unbound, serum-free corticosterone levels were measured using a commercially available sandwich ELISA designed to specifically detect active (but not inactive 11DHC) steroid (cat no: KGE009, R&D systems, Abingdon, UK). Serum was analysed in accordance with the manufacturer’s instructions and data expressed as nanogrammes per millilitre (ng/ml). Serum P1NP was determined using a commercially available sandwich ELISA (cat no: AC-33F1, Immunodiagnostic Systems, Tyne & Wear, UK) in accordance with the manufacturer’s instructions and data expressed as ng/ml. Serum CTX-1 was determined using a commercially available sandwich ELISA (cat no: AC-06F1, Immunodiagnostic Systems, Tyne & Wear, UK) in accordance with the manufacturer’s instructions and data expressed as units per microlitre.

Fenton C.G., Doig C.L., Fareed S., Naylor A., Morrell A.P., Addison O., Wehmeyer C., Buckley C.D., Cooper M.S., Lavery G.G., Raza K, & Hardy R.S. (2019). 11β-HSD1 plays a critical role in trabecular bone loss associated with systemic glucocorticoid therapy. Arthritis Research & Therapy, 21, 188.

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Plasma Bone Turnover Markers

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Blood was collected in heparin‐coated tubes and centrifuged (2000g; 10 minutes; RT) to collect the supernatant (plasma). N‐terminal propeptide of type I procollagen (PINP) (cat. AC‐33F1; Immunodiagnostic Systems, Boldon, UK) and C‐terminal telopeptides of type I collagen (CTX‐I) ELISAs (AC‐06F1; Immunodiagnostic Systems) were performed according to the manufacturer's instructions.

Ahmad M., Stirmlinger N., Jan I., Stifel U., Lee S., Weingandt M., Kelp U., Bockmann J., Ignatius A., Böckers T.M, & Tuckermann J. (2022). Downregulation of the Autism Spectrum Disorder Gene Shank2 Decreases Bone Mass in Male Mice. JBMR Plus, 7(2), e10711.

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Bone Remodeling Marker Quantification

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Bone remodeling markers, N-terminal propeptide (P1NP) and tartrate-resistant acid phosphatase (TRAP) were measured with an ELISA kit [cat. AC-33F1 for P1NP and cat SB-TR103 for TRAP, Immunodiagnostic Systems, Boldon Colliery, UK] according to manufacturer's instructions.

Gozes I., Bardea A., Reshef A., Zamostiano R., Zhukovsky S., Rubinraut S., Fridkin M, & Brenneman D.E. (1996). Neuroprotective strategy for Alzheimer disease: intranasal administration of a fatty neuropeptide.. Proceedings of the National Academy of Sciences of the United States of America, 93(1), 427-432.

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Bone Remodeling Marker Quantification

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Palmieri M., Joseph T.E., O'Brien C.A., Gomez-Acevedo H., Kim H.N., Manolagas S.C, & Ambrogini E. (2023). Deletion of the scavenger receptor Scarb1 in myeloid cells does not affect bone mass. Bone, 170, 116702.

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Rodent Bone Metabolism Markers

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Rodent-specific ELISAs were used to measure serum C-terminal telopeptide of collagen (CTX) as a bone resorption marker and serum procollagen type I N-terminal propeptide (PINP) as a bone formation marker (AC-06F1 and AC-33F1, Immunodiagnostic Systems, Tyne and Wear, Boldon Colliery, UK) following the manufacturer’s protocol.

Kosaka S., Nadatani Y., Higashimori A., Otani K., Fujimoto K., Nagata Y., Ominami M., Fukunaga S., Hosomi S., Kamata N., Tanaka F., Nagami Y., Taira K., Imoto S., Uematsu S., Watanabe T, & Fujiwara Y. (2021). Ovariectomy-Induced Dysbiosis May Have a Minor Effect on Bone in Mice. Microorganisms, 9(12), 2563.

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Serum Biomarkers for Bone Turnover

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Terminal serum samples were collected at sacrifice and stored at -80C. Serum N-terminal propeptide of type I procollagen (P1NP) (catalog #AC-33F1, Immunodiagnostic Systems Inc.) and C-telopeptide fragments (CTX) (catalog #AC-06F1, Immunodiagnostic Systems Inc.,) were measured as suggested by the manufacturer and previously described^{85 (link),86 (link)}.

Dudakovic A., Jerez S., Deosthale P.J., Denbeigh J.M., Paradise C.R., Gluscevic M., Zan P., Begun D.L., Camilleri E.T., Pichurin O., Khani F., Thaler R., Lian J.B., Stein G.S., Westendorf J.J., Plotkin L.I, & van Wijnen A.J. (2022). MicroRNA-101a enhances trabecular bone accrual in male mice. Scientific Reports, 12, 13361.

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