Thiol poly ethylene glycol amine sh peg nh2

Hydrogen tetrachloroaurate trihydrate (HAuCl₄·3H₂O; 99%), cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH₄; 99%), silver nitrate (AgNO₃; 99%), L-ascorbic acid (AA), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimidehydrochloride (EDC), sodium oleate (NaOL; >97%), hydrochloric acid (HCl, 37%), (3-mercaptopropyl)trimethoxysilane (MPTMS), ethylenediaminetetraacetic acid (EDTA), poly(ethylene glycol) methyl ether thiol (PEG-SH, M_w ≈ 5000), goat anti-human IgG, dithiotreitol (DTT), thiol-poly(ethylene glycol)amine (SH-PEG-NH₂), and human serum IgG were provided by Sigma–Aldrich (St. Louis, MO). Traut’s reagent, Zeba^TM spin desalting columns (7K MWCO), and Ellman’s reagent were purchased from Thermo Scientific (Rockford, IL). PEG6-NHNH2 was obtained from SensoPath Technologies (Bozeman, MT). Glass slides (7 mm × 50 mm × 0.7 mm) were from Delta Technologies (Loveland, CO).

Trisodium citrate dehydrate (C₆H₅O₇Na₃ ·2H₂O or NaCt), silver nitrate (AgNO₃), tetrachloroauric acid tetrahydrate (HAuCl₄^.4H₂O; 99.99% trace metals basis), Thiol-polyethylene glycol-amine (SH-PEG-NH₂, molecular weight 2 kDa), fetal bovine serum (FBS), phosphate buffered saline (PBS) and trypsin were purchased from Sigma Aldrich^® LLC, St. Louis, MO, USA; Roswell Park Memorial Institute (RPMI) media and Minimum Essential Medium (MEM) were purchased from Biowest^®; and PrestoBlue™ cell viability reagent (PB) were obtained from Invitrogen Co. (Scotland, UK); Annexin V-CF Blue 7-AAD apoptosis staining/detection kit (ab214663) from Abcam; DCFDA/H2DCFDA—Cellular ROS Assay Kit (D399) from Invitrogen; and QIAzol from Qiagen.

Fetal bovine serum (FBS), phosphate-buffered saline (PBS), trypsin-EDTA, antibiotic antimycotic solution, thiol-polyethylene glycol-amine (SH-PEG-NH2), molecular weight 2kDa, trisodium citrate dehydrate (C₆H₅O₇Na₃·2H₂O or NaCt), tetrachloroauric acid tetrahydrate (HAuCl₄·4H₂O, 99.99%), silver nitrate (AgNO₄), and L-ascorbic acid, ≥99% were purchased from Sigma Aldrich^® LLC (St. Louis, MO, USA). Roswell Park Memorial Institute (RPMI-1640) media and Minimum Essential Medium (MEM) were purchased from Biowest^® (Nuaillé, France). QIAzol lysis reagent was purchased from QIAGEN Inc. (Valencia, CA, USA). Norgen’s MicroScript microRNA cDNA Synthesis Kit was purchased from Norgen BioteK (Thorold, ON, Canada).