The LV tissue was lysed in radioimmunoprecipitation assay (RIPA) lysis buffer, and the total protein was extracted and detected with a BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA). Approximately 30 μg of total protein was separated by electrophoresis on Laemmli sodium dodecyl sulfate (SDS) polyacrylamide gels. After electrophoresis, the samples were transferred to Immobilon-FL PVDF membranes (Millipore, USA). The membranes were blocked with 5% nonfat milk and then incubated with anti-IL-22, anti-IL-22R1 (both from Abcam, Cambridge, England), anti-atrial natriuretic peptide (ANP), anti-B- type natriuretic peptide (BNP), anti-β-myosin heavy chain (β-MHC, triple from Santa Cruz, Dallas, TX, USA), anti-STAT3, anti-phospho-STAT3, anti-ERK, anti-phospho-ERK, anti-c-Jun N-terminal kinase (JNK), anti-phospho-JNK, anti-P38, anti-phospho-P38, and anti- glyceraldehyde-3-phosphate dehydrogenase (GAPDH, nine above from Cell Signaling Technology, Boston, USA) antibodies at 4°C overnight. The secondary antibodies were incubated at room temperature for 1 hour. The blots were scanned using a two- color infrared imaging system (Odyssey; LI- COR Biosciences, Lincoln, NE, USA).
Anti c jun n terminal kinase
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-c-Jun N-terminal kinase is a laboratory reagent that detects the c-Jun N-terminal kinase (JNK) protein. JNK is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in cellular processes such as cell growth, differentiation, and apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Anti erk, by Cell Signaling Technology (1 mentions)
Anti phospho erk, by Cell Signaling Technology (1 mentions)
Anti p38, by Cell Signaling Technology (1 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Immobilon fl pvdf membrane, by Merck Group (1 mentions)
Anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (1 mentions)
Anti stat3, by Santa Cruz Biotechnology (1 mentions)
Anti phospho jnk, by Cell Signaling Technology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
Anti phospho stat3, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Enzyme linked immunosorbent assay elisa kits for pge2, by R&D Systems (1 mentions)
Anti phosphorylated iκbα, by Cell Signaling Technology (1 mentions)
Anti phosphorylated erk1 2, by Cell Signaling Technology (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Anti phosphorylated jnk, by Cell Signaling Technology (1 mentions)
2 protocols using anti c jun n terminal kinase
1
LV Protein Expression Analysis
2
Anti-inflammatory Mechanisms of Bioactive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DMC (Figure 1A(Fig. 1) ), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were obtained from Invitrogen-Gibco (Grand Island, NY, USA). Enzyme-linked immunosorbent assay (ELISA) kits for PGE2, TNF-α, IL-1ß, and IL-6 were purchased from R&D Systems, Inc. (St. Louis, MO, USA). Anti-iNOS (mouse), Anti-COX-2 (rabbit), Anti-IκB-α (mouse), anti-phosphorylated IκB-α (rabbit, anti-p-IκB-α), anti-c-Jun N-terminal kinase (rabbit, JNK), anti-phosphorylated JNK (rabbit, anti-p-JNK), anti-extracellular signal-regulated kinases (rabbit, ERK1/2), anti-phosphorylated ERK1/2 (rabbit, anti-p-ERK1/2), anti-p38 (rabbit), and anti-phosphorylated p38 (rabbit, anti-p-p38) mouse or rabbit antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). All other reagents were purchased from the Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).
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