Nci h1299

Normal human lung epithelial cells BEAS-2B (iCell-h023) and NSCLC cell lines A549 (iCell-h011), NCI–H1299 (iCell-h153), NCI–H1650 (iCell-h155-001b), and NCI–H1975 (iCell-h156) were obtained from iCell (Shanghai, China). They were cultivated in Dulbecco's modification of Eagle's medium Dulbecco (DMEM) (iCell-0001), F–12K (iCell-0007), or RPMI-1640 (iCell-0002) media supplemented with 10% phosphate buffer saline (PBS) and 100 U/mL penicillin-streptomycin.

A total of 60 paired LC and adjacent normal tissues were collected from Jan 2020 to Jun 2020 at The Fourth Hospital of Hebei Medical University (Shijiazhuang, China). All experiments were approved by the Ethics Committee of The Fourth Hospital of Hebei Medical University (2018MEC160), and written informed consent was obtained from all patients. Patients diagnosed with recurrence and those who received preoperative radiotherapy, chemotherapy, or biotherapy were excluded from the study.
The human bronchial epithelial cell line (HBE) was purchased from CHI Scientific, Inc. The PLA-801D, A549, NCI-H1299, HCC827 and NCI-H1437 cell lines were purchased from iCell Bioscience Inc. The A549 cells were cultured in F-12K medium (Procell Life Science & Technology Co., Ltd.) containing 10% fetal bovine serum (FBS, Zhejiang Tianhang Biotechnology Co., Ltd.). The other cell lines were maintained in RPMI-1640 medium (Beijing Solarbio Science & Technology, Co., Ltd.) supplemented with 10% FBS. All cell lines were incubated at 37˚C in a humidified atmosphere containing 5% CO₂.