Mouse livers were isolated and fixed in 4% paraformaldehyde (PFA), then embedded in paraffin and sectioned. Sections were then deparaffinized using heat and xylene. Tissues were rehydrated using reducing concentrations of ethanol, then finally in water. Slides were stained with eosin and counterstained with hematoxylin (Thermo Fisher Scientific, Waltham, MA, USA). The sections were dehydrated using increasing concentrations of ethanol, cleared using xylene, and finally mounted using Paramount mounting medium (Thermo Fisher Scientific, Fisher Scientific, Waltham, MA, USA). Images were viewed and recorded on a Nikon Eclipse E800 Core microscope (Nikon, Melville, NY, USA). Optimal cutting temperature (OCT) compound blocks were prepared by embedding livers in OCT compound (Sakura Finetek USA, Torrance, CA, USA). Slides were fixed with 4% PFA at 4 °C for 10 min, then stained with oil Red O solution for 120 min at room temperature. Liver sections were counterstained with hematoxylin (Carolina Biological Supply Company, Burlington, NC, USA).
Eclipse e800 core microscope
Manufactured by Nikon
Sourced in United States
The Nikon Eclipse E800 Core microscope is a high-performance laboratory instrument designed for a wide range of scientific applications. It features a modular design, allowing for customization to meet specific research needs. The microscope provides superior optical performance, delivering clear and detailed images for accurate sample analysis.
Automatically generated - may contain errors
2 protocols using eclipse e800 core microscope
1
Histological Analysis of Mouse Livers
2
Histological Analyses of Mouse Liver and Adipose
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Mouse livers and epididymal adipose were isolated and fixed in 4% PFA, then embedded in paraffin, and sectioned. Sections were then deparaffinized using heat and xylene. Tissues were rehydrated using reducing concentrations of ethanol, then finally in water. Slides were stained with eosin and counterstained with hematoxylin (Thermo Fisher Scientific, Waltham, MA, USA). The sections were dehydrated using increasing concentrations of ethanol, cleared using xylene, and finally mounted using Paramount mounting medium (Thermo Fisher Scientific, Waltham, MA, USA). The images were viewed and recorded on a Nikon Eclipse E800 Core microscope (Nikon, Melville, NY, USA).
OCT blocks were prepared by embedding livers in OCT compound (Sakura Finetek USA, Torrance, CA, USA). Slides were fixed with 4% PFA at 4 °C for 10 min, then stained with oil Red O solution for 120 min. The liver sections were counterstained with hematoxylin (Carolina Biological Supply Company, Burlington, NC, USA).
OCT blocks were prepared by embedding livers in OCT compound (Sakura Finetek USA, Torrance, CA, USA). Slides were fixed with 4% PFA at 4 °C for 10 min, then stained with oil Red O solution for 120 min. The liver sections were counterstained with hematoxylin (Carolina Biological Supply Company, Burlington, NC, USA).
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