The collected serum samples underwent laboratory analyses, which included collection of the following: complete metabolic panel (CMP), complete blood count (CBC), inflammatory markers (c reactive protein [CRP], immunoglobulins A [IgA], G [IgG], and M [IgM]), and cytokine assays. ELISA was used to assess plasma levels of the cytokine IL-22 (BMS2047, Invitrogen) and high-sensitivity ELISA was used to measure plasma IL-17 (BMS2017HS, Invitrogen) per manufacturer’s instructions; plasma was diluted 1:2 for both respective assays. Results were read on a Spectra Max Plus 384 plate reader and analyzed using SoftMax Pro software (Molecular Devices, San Jose, CA). Other cytokines mentioned were measured using Luminex platforms.
Bms2047
Manufactured by Thermo Fisher Scientific
The BMS2047 is a compact and portable benchtop centrifuge designed for a variety of laboratory applications. It features a maximum speed of 6,000 rpm and can accommodate rotor sizes up to 12 x 1.5/2.0 mL tubes. The centrifuge is equipped with a brushless motor and digital speed control for precise speed regulation. It also includes a safety lid interlock and automatic rotor recognition for user convenience and safety.
Automatically generated - may contain errors
2 protocols using bms2047
1
Evaluating Plasma Cytokine Profiles
2
Cytokine Expression Profiling in Vitiligo
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ELISA was performed to measure the protein expression levels of cellular cytokines (IFN-γ, IL-17A and IL-22) in the peripheral blood of patients with VIT and of healthy individuals, or from CD4+T cells or melanocyte supernatant. The PBMCs and supernatant were separated by centrifugation at 2,000 × g for 15 min at 4°C and stored in aliquots at 80°C. The expression levels of inflammatory factors (IFN-γ, IL-17A and IL-22) in CD4+T cells or melanocyte supernatant were determined using their corresponding ELISA kits (cat. nos. KHC4021 for IFN-γ, BMS2017 for IL-17A and BMS2047 for IL-22; Invitrogen; Thermo Fisher Scientific, Inc.). OD values were detected and measured using a Crocodile 4-in-one assay miniWorkstation (Titertek-Berthold).
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