Poly l lysine p8920

Manufactured by Merck Group

Sourced in Japan, Sao Tome and Principe

Poly-l-lysine (P8920) is a water-soluble, cationic polymer that is widely used in various research applications. It is composed of the amino acid lysine in its L-enantiomeric form. Poly-l-lysine has the ability to adhere to a variety of surfaces, making it a useful tool in cell culture, microscopy, and other laboratory techniques.

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Lab products found in correlation

Live dead baclight bacterial viability kit for microscopy, by Thermo Fisher Scientific (1 mentions) Bz 8000, by Keyence (1 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) β mercaptoethanol, by Thermo Fisher Scientific (1 mentions) Acrylamide bis solution, by Bio-Rad (1 mentions) Alexa fluor 488 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Bio-Rad (1 mentions) Peroxidase conjugated anti rabbit igg, by GE Healthcare (1 mentions) Alexa fluor 488 conjugated lectin pna, by Thermo Fisher Scientific (1 mentions) Peroxidase conjugated anti mouse igg, by Jackson ImmunoResearch (1 mentions)

2 protocols using poly l lysine p8920

Bacterial Live/Dead Staining Enumeration

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Living and dead cells were enumerated microscopically after live/dead staining (LIVE/DEAD BacLight Bacterial Viability Kit for microscopy; Thermo Fisher Scientific), as described previously (29 (link)). Briefly, cells on slant medium were suspended in 0.85% NaCl, collected by centrifugation at 10,000×g for 15 min, and then rinsed twice as described. The pellet was resuspended in 0.85% NaCl and Live/Dead reagent was added to 1 mL of the diluted cell suspension. After 15 min, the cell suspension was filtered onto a polycarbonate black filter (0.2 μm pore size and 25 mm in diameter; Advantec Toyo Kaisha, Tokyo, Japan) treated with 0.01% poly-l-lysine (P8920; Sigma-Aldrich). The cell number was enumerated under a fluorescence microscope (BZ-8000; KEYENCE, Osaka, Japan).

Ogawa T., Hattori S., Kamezaki K., Kato H., Yoshida N, & Katayama Y. (2017). Isotopic Fractionation of Sulfur in Carbonyl Sulfide by Carbonyl Sulfide Hydrolase of Thiobacillus thioparus THI115. Microbes and Environments, 32(4), 367-375.

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Immunofluorescence Analysis of TSSK3 in Cells

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Reagents were obtained from various sources and details are provided below, under the subsections pertaining to each experimental procedure. Bovine serum albumin (A-7906, for blocking non-specific binding), Tween-20 (P-7949), Immobilon-P PVDF membrane (Millipore) and poly-L-lysine (P-8920) were purchased from Sigma-Aldrich (St. Louis, MO). Paraformaldehyde (16% solution, EM Grade), and β-mercaptoethanol were purchased from Fisher Scientific (Waltham, MA). Triton X-100 and 30% acrylamide/Bis solution were obtained from BioRad. Peroxidase-conjugated anti-mouse IgG (Jackson Immunoresearch, West Grove, PA) and peroxidase-conjugated anti-rabbit IgG (GE Healthcare, Pittsburg, PA) were used. AlexaFluor488-conjugated PNA lectin, AlexaFluor488-conjugated anti-rabbit IgG, Hoechst 333452 and gentamicin (10 mg/ml) were purchased from Invitrogen (ThermoFisher Scientific, Grand Island, NY). Anti-TSSK3 rabbit polyclonal antibodies AP7246a and AP17810b were obtained from Abcepta (San Diego, CA). A rabbit polyclonal antibody against alpha/beta-tubulin was obtained from Cell Signaling Technology (Danvers, MA).

Nayyab S., Gervasi M.G., Tourzani D.A., Caraballo D.A., Jha K.N., Teves M.E., Cui W., Georg G.I., Visconti P.E, & Salicioni A.M. (2021). TSSK3, a novel target for male contraception, is required for spermiogenesis.. Molecular reproduction and development, 88(11), 718-730.

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