P23ey05607

Manufactured by BD

The P23EY05607 is a laboratory equipment product manufactured by BD. It is designed to perform specific functions in a laboratory setting. No further details about its core function or intended use can be provided in an unbiased and factual manner without the risk of extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

C355 gal4, by BD (2 mentions) Mgst1kg04713, by BD (2 mentions) Su p ey13245, by BD (2 mentions) Akr1bpl00034, by BD (2 mentions) Akr1bey07011, by BD (2 mentions) P23 rnai hmj24151, by BD (2 mentions) P23 rnai 2 gl01292, by BD (2 mentions) Uas dicer2, by BD (2 mentions) Actin5c gal4, by BD (1 mentions) C306 gal4, by BD (1 mentions)

2 protocols using p23ey05607

Drosophila Genetics and Immunofluorescence

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fly stocks were maintained on cornmeal/agar/yeast food at 21°C, except where noted. Before immunofluorescence staining, newly eclosed flies were fed wet yeast paste every day for 2–4 days. Unless specified, yw (BDSC 1495) was used as the control. The following stocks were obtained from the Bloomington Drosophila Stock Center: c355 GAL4 (BDSC 3750), actin-5C GAL4 (BDSC 8807), mgst1^KG04713 (BDSC 13839), Su(P)^EY13245 (BDSC 20866), p23^EY05607(BDSC 16661), akr1B^PL00034 (BDSC 19594), akr1B^EY07011 (BDSC 16777), p23 RNAi HMJ24151 (BDSC 62911), p23 RNAi-2 GL01292 (BDSC 41862), akr1B deficiency-1 DF(3L)BSC577 (BDSC 25411), akr1B deficiency-2 DF(3L)ED4475 (BDSC 8069), akr1B RNAi HMS05657 (BDSC 67838), akr1B RNAi-2 HMC05226 (BDSC 62219) and UAS Dicer 2 (BDSC 24651). The following stocks were obtained from the Exelexis Stock Center: mgst1^d10243, akr1B^d00405 and pxt^f01000 (Thibault et al., 2004 (link)). The oskar GAL4 line (second chromosome; BDSC 44241) was a generous gift from Anne Ephrussi [European Molecular Biology Laboratory; (Telley et al., 2012 (link))]. Expression of the RNAi lines were achieved by crossing to actin-5C GAL4, c355 GAL4 or oskar GAL4, maintaining fly crosses at 21°C and maintaining progeny at 29°C for 5–6 days. UAS Dicer was used in combination with c355 to enhance RNAi efficiency where noted in the figure legends.

Mellentine S.Q., Brown H.N., Ramsey A.S., Li J, & Tootle T.L. (2024). Specific prostaglandins are produced in the migratory cells and the surrounding substrate to promote Drosophila border cell migration. Frontiers in Cell and Developmental Biology, 11, 1257751.

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Drosophila Genetics: Protocols and Stock Utilization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fly stocks were maintained on cornmeal/agar/yeast food at 21°C, except where noted. Before immunofluorescence staining, newly eclosed flies were fed wet yeast paste every day for 2–4 days. Unless specified, yw (BDSC 1495) was used as the control. The following stocks were obtained from the Bloomington Drosophila Stock Center: c355 GAL4 (BDSC 3750), c306 GAL4 (BDSC 3743), mgst1^KG04713 (BDSC 13839), Su(P)^EY13245 (BDSC 20866), p23^EY05607(BDSC 16661), akr1B^PL00034 (BDSC 19594), akr1B^EY07011 (BDSC 16777), p23 RNAi HMJ24151 (BDSC 62911), p23 RNAi-2 GL01292 (BDSC 41862), akr1B RNAi HMS05657 (BDSC 67838), akr1B RNAi-2 HMC05226 (BDSC 62219) and UAS Dicer 2 (BDSC 24651). The following stocks were obtained from the Exelexis Stock Center: mgst1^d10243, akr1B^d00405 and pxt^f01000 (Thibault et al., 2004 (link)). The oskar GAL4 line (second chromosome; BDSC 44241) was a generous gift from Anne Ephrussi (European Molecular Biology Laboratory; (Telley et al., 2012 (link))). Expression of the RNAi lines were achieved by crossing to c355 GAL4 or oskar GAL4, maintaining fly crosses at 21°C and maintaining progeny at 29°C for 5–6 days. UAS Dicer was used in combination with c355 to enhance RNAi efficiency were noted in the figure legends.

Mellentine S.Q., Ramsey A.S., Li J., Brown H.N, & Tootle T.L. (2023). Specific prostaglandins are produced in the migratory cells and the surrounding substrate to promote Drosophila border cell migration. bioRxiv.

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