Rabbit anti sma

Manufactured by Abcam

Sourced in United States, China

Rabbit anti-SMA is a primary antibody that recognizes the alpha-smooth muscle actin (SMA) protein. SMA is a cytoskeletal protein found in smooth muscle cells and is commonly used as a marker for these cell types.

Automatically generated - may contain errors

Lab products found in correlation

Enhanced ecl substrate, by Thermo Fisher Scientific (2 mentions) Donkey anti goat conjugated to fitc, by Abcam (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (2 mentions) Rabbit anti aqp4, by Merck Group (1 mentions) Mouse anti glut1, by Abcam (1 mentions) Rabbit anti gfap, by Agilent Technologies (1 mentions) Bromodeoxyuridine (brdu), by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Brdu antibody, by Roche (1 mentions) Vimentin, by Merck Group (1 mentions) Donkey antimouse alexafluor 488, by Abcam (1 mentions) Conjugated vimentin cy3, by Merck Group (1 mentions) Alexa fluor 594 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) Rabbit anti connexin 43 cx 43, by Abcam (1 mentions) Mouse anti α actinin, by Abcam (1 mentions) Alexa fluor 660 phalloidin, by Thermo Fisher Scientific (1 mentions) Potassium chloride (kcl), by Merck Group (1 mentions) Fluoview 1000 laser scanning confocal microscope, by Olympus (1 mentions) Imaging system, by Bio-Rad (1 mentions) Polyvinylidene fluoride (pvdf), by Merck Group (1 mentions)

10 protocols using rabbit anti sma

Immunolabeling of Brain Tissue Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Free-floating brain sections were permeabilized and blocked for 45 min at 4 °C in a solution of 1% BSA, 0.5% Triton X-100 and 5% normal donkey serum in PBS. Primary antibodies (rabbit anti-AQP4, 1:500, MerckMillipore; rabbit anti-GFAP, 1:500, Agilent Technologies; mouse anti-GLUT1, 1:250, Abcam, rabbit anti-SMA, 1:500, AbCam) were added in PBS and incubated overnight at 4 °C on a rocking table. After 3x10 min washes in PBS at room temperature secondary antibodies (Alexa-Fluor 488- and 568-conjugated secondary antibodies, 1:1000) in PBS were added for 90 minutes at 4 °C on a rocking table. Slices were then washed in PBS with DAPI (1:1000) and/or tomato lectin (Lycoperiscon esculentum, 1:20, SigmaAldrich) for 20 minutes, washed again and mounted.

Bèchet N.B., Shanbhag N.C, & Lundgaard I. (2021). Glymphatic pathways in the gyrencephalic brain. Journal of Cerebral Blood Flow & Metabolism, 41(9), 2264-2279.

+ Open protocol

+ Expand

Multimodal Tissue Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cryo-preserved methanol/acetone fixed tissue was stained for proliferating cells using a BrdU antibody (Roche Diagnostics, Mannheim, Germany). Prior to sacrifice, BrdU (100 mg/kg, Sigma-Aldrich, Taufkirchen, Germany) was injected intraperitoneally. Depending on the proliferation rate of the tumors, BrdU circulation time was 16 h for H-, 8 h for HI-, and 4 h for AT1-tumors. To detect hypoxic areas in tumors, 60 mg/kg pimonidazole (Hypoxyprobe™−1, NPI, Inc., Burlington, MA, USA) was injected intravenously 1 h before sacrificing. Cell nuclei were counterstained with DAPI (Invitrogen Molecular Probes, Eugene, USA). Vascular structures (mouse anti-rat CD31-antibody (Chemicon-Merck-Millipore, Billerica, MA, USA) and pericytes (rabbit anti-SMA (Abcam, Cambridge, UK) were double stained and visualized using fluorochrom-labelled antibodies (see Supplement).

Glowa C., Karger C.P., Brons S., Zhao D., Mason R.P., Huber P.E., Debus J, & Peschke P. (2016). Carbon ion radiotherapy decreases the impact of tumor heterogeneity on radiation response in experimental prostate tumors. Cancer letters, 378(2), 97-103.

+ Open protocol

+ Expand

Cardiac Tissue Immunofluorescence Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The tissues
were fixed with 4% paraformaldehyde, permeablized by 0.25% Triton
X-100, and blocked by 5% bovine serum albumin (BSA). Immunostaining
was performed using the antibodies mouse anti-α-actinin (Abcam;
1:200), rabbit anti-SMA (Abcam, 1:200), Vimentin (Sigma, 1:200), mouse
antitype I collagen (GeneTex, 1:200), and rabbit anti-connexin 43
(Cx-43) (Abcam; 1:200) and the secondary antibodies donkey antimouse-Alexa
Fluor 488 (Abcam; 1:400) and donkey antirabbit-Alexa Fluor 594 (Life
Technologies; 1:200). Phalloidin-Alexa Fluor 660 (Invitrogen; 1:200)
was used to stain F-actin fibers. Conjugated Vimentin-Cy3 (Sigma;
1:200) was used to stain for Vimentin. Confocal microscopy images
were obtained using an Olympus FluoView 1000 laser scanning confocal
microscope (Olympus Corporation). Cardiomyocytes and fibroblasts were
quantified by the average number of α-actinin or Vimentin stained
cells divided by the total cell number based on DAPI counterstain
(n = 3). The 100 mM potassium chloride (Sigma) was
used to relax the tissue prior to fixation for sarcomere length measurement.
The respective sarcomere length in normal and fibrotic tissues stained
with sarcomeric α-actinin was measured following imaging.

Wang E.Y., Rafatian N., Zhao Y., Lee A., Lai B.F., Lu R.X., Jekic D., Davenport Huyer L., Knee-Walden E.J., Bhattacharya S., Backx P.H, & Radisic M. (2019). Biowire Model of Interstitial and Focal Cardiac Fibrosis. ACS Central Science, 5(7), 1146-1158.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Equal amounts of protein per lane (30 μg) were subjected to electrophoresis on a 12% SDS-PAGE gel. The proteins were electrotransferred onto a polyvinylidene difluoride membrane (PVDF, Millipore, Billerica, MA, United States). The membrane was blocked with 5% non-fat dry milk/0.1% Tween-20 in Tris-buffered saline for 1 h at room temperature. Thereafter, the membrane was incubated with different primary antibodies, including rabbit anti-MLCK (1:5000 dilution, Abcam), rabbit anti-SMA (1:200 dilution, Abcam) and rabbit anti-cleaved caspase-3 (1:1000 dilution, Cell Signaling Technology). Subsequently, the membrane was treated with secondary antibody for 2 h at room temperature. Immunoblots were probed using enhanced ECL substrate (Thermo, Rockford, IL, United States). The chemiluminescence level was recorded using an imaging system (Bio-Rad, Hercules, CA, United States). The results were normalized to β-actin.

Song Y., Liu P., Li Z., Shi Y., Huang J., Li S., Liu Y., Zhang Z., Wang Y., Zhu W, & Yang G.Y. (2018). The Effect of Myosin Light Chain Kinase on the Occurrence and Development of Intracranial Aneurysm. Frontiers in Cellular Neuroscience, 12, 416.

+ Open protocol

+ Expand

Penile Tissue Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cellular protein samples were prepared by homogenization of penile tissue in a lysis buffer containing 1% IGEPAL CA-630, 0.5% sodium deoxycholate, 0.1% sodium docecyl sulfate, aprotinin (10 mg/mL), leupeptin (10 mg/mL), and phosphate buffered saline. The cellular lysates from penis containing 20 mg of protein were electrophoresed in sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene fluoride membrane (Millipore Corp.). Primary antibodies were rabbit anti-⍺-SMA (1:1000; Abcam), rabbit anti- nNOS (1:400; Abcam) and rabbit anti-GAPDH (1:2000; cwBio, Lot:01225/50,404). After the hybridization of secondary antibodies, the resulting images were analyzed with ChemiImager 4000 (Alpha Innotech Corporation, San Leandro, CA, USA).

Xu Y., Yang Y., Zheng H., Huang C., Zhu X., Zhu Y., Guan R., Xin Z., Liu Z, & Tian Y. (2020). Intracavernous injection of size-specific stem cell spheroids for neurogenic erectile dysfunction: Efficacy and risk versus single cells. EBioMedicine, 52, 102656.

+ Open protocol

+ Expand

Clearing and Staining Duodenal Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were sacrificed and their duodenums dissected and fixed at 4%PFA for 24 h while shaking at 4 °C. Following washes with PBS for additional 24 h at 4 °C, tissue was incubated in hydrogel solution (according to manufacturer’s protocol) for 24 h at 4 °C. Following a further 3 h incubation at 37 °C, the hydrogel-embedded tissue was placed in an X-CLARITY ETC chamber (LOGOS Biosystems) for electrophoretic tissue clearing for 7 h. The cleared duodenum was immunostained with goat anti-PDGFRa (1:100), chicken anti-GFP (1:100), rat anti-c-KIT (1:200), goat anti-GFP (1:500), or rabbit anti-SMA (Abcam 1:600). Primary and secondary antibodies were incubated for 48 h at 4 °C. The stained intestine was placed en bloc on an image slide using a 0.5 mm depth adhesive silicone isolator, mounted in X-CLARITY mounting solution and imaged using confocal scanning. Z-stack projections were compiled.

Shushan M, & Shoshkes-Carmel M. (2022). Lineage Tracing of FOXL1+ Cells in the Tunica Muscularis Suggests Mutual Origin for Telocytes and Smooth Muscle Cells. Life, 12(2), 176.

+ Open protocol

+ Expand

In Vivo Assessment of EPC Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols

Our first step, in vivo, was to establish that EPCs from the construct had the ability to migrate from the Construct into the myocardium. In order to assess EPC migration, eGFP⁺ EPCs from transgenic rats were utilized to create the Construct (20 mg/ml Fibrin, 17 × 10⁶ EPCs/ml). This Construct was then implanted onto ischemic myocardium following LAD ligation. The hearts were explanted 1 week following implant, flushed with PBS, and distended with OCT embedding compound (Electron Microscopy Sciences). Visualization was performed in the peri-infarct borderzone, which was defined as one microscopic field from the infarct. Hearts treated with Construct were compared to IC. Sections were briefly washed, then fixed in 4% paraformaldehyde and then stained for anti-GFP and anti-α smooth muscle actin (SMA, pericytes). Primary antibodies used for indirect immunofluorescence were goat anti-GFP (1:200, Rockland) and rabbit anti-SMA (1:150, Abcam). Secondary antibodies included donkey anti-goat conjugated to FITC (1:200, Abcam), and donkey anti-rabbit conjugated to Alexa Fluor 594 (1:200, Abcam). Nuclei were counterstained with DAPI (Vector Laboratories). Construct was compared to Control (isolated LAD ligation) and IC.

Atluri P., Miller J.S., Emery R.J., Hung G., Trubelja A., Cohen J.E., Lloyd K., Han J., Gaffey A.C., MacArthur J.W., Chen C.S, & Woo Y.J. (2014). Tissue Engineered, Hydrogel-Based Endothelial Progenitor Cell Therapy Robustly Revascularizes Ischemic Myocardium and Preserves Ventricular Function. The Journal of thoracic and cardiovascular surgery, 148(3), 1090-1098.

+ Open protocol

+ Expand

Immunofluorescent Vascular Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sections were briefly washed three times in PBS, fixed in 4% paraformaldehyde for 10 minutes at room temperature, and blocked in 10% Fetal Bovine Serum (FBS) (Gibco) for 1 hour at 37°C. Primary antibodies were diluted 1:150 in PBS and incubated for 2 hours at 37°C. Secondary antibodies were diluted in PBS and incubated for 1 hour at 37°C. Primary antibodies included sheep anti-vWF (endothelial cells of vasculature) conjugated to FITC (Abcam) and rabbit anti-SMA (Abcam). Secondary antibodies (1:200) were donkey anti-goat conjugated to FITC (Abcam), and donkey anti-rabbit conjugated to Alexa Fluor 594 (Abcam). Nuclei were stained with DAPI (Vector Laboratories). Vasculature was quantified using ImageJ (NIH). Measurements were made 1 high power field from the infarct at 20× magnification.

+ Open protocol

+ Expand

Protein Expression Analysis of Rat Aortic SMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were lysed from rat aortic SMCs, and equal amount of protein per lane (20 μg) was separated by 12.5% SDS‐PAGE gel (Epizyme, China). Proteins were electrotransferred onto a polyvinylidene difluoride (PVDF) membrane (MilliporeSigma). The membrane was blocked with a QuickBlock blocking buffer (Beyotime Biotechnology) for 15 minutes at room temperature, followed by incubation with different primary antibodies, including rabbit anti‐MYLK (1:5000 dilution; Abcam), rabbit anti‐COL4A2 (1:500 dilution; Bioss, China), rabbit anti‐SMA (1:100 dilution; Abcam), goat anti‐ transgelin (TAGLN, 1:500 dilution; Abcam), and mouse anti‐VCL (1:1000 dilution; Millipore) overnight. The membrane was then incubated with horseradish peroxidase‐conjugated secondary antibody for 1 h at room temperature. Immunoblots were probed using enhanced ECL substrate (Thermo). The blot was detected using an imaging system (Bio‐Rad), and the chemiluminescence level was recorded. The results were normalized to GAPDH. The experiments were replicated for three times.

Liu Y., Song Y., Liu P., Li S., Shi Y., Yu G., Quan K., Fan Z., Li P., An Q, & Zhu W. (2021). Comparative bioinformatics analysis between proteomes of rabbit aneurysm model and human intracranial aneurysm with label‐free quantitative proteomics. CNS Neuroscience & Therapeutics, 27(1), 101-112.

+ Open protocol

+ Expand

Immunofluorescence Analysis of Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunofluorescence, TSC-patient-specific and unaffected control iPSCs, pNSCs, neurons, and astrocytes were fixed in 4% (w/v) paraformaldehyde at room temperature for 15 min, permeabilized for 15 min in PBS containing 0.5% (v/v) Triton X-100, blocked with 5% (w/v) BSA for 1 hr at room temperature, and incubated with primary antibodies overnight at 4°C in PBS containing 1% (w/v) BSA. Cells were then washed three times in PBS and incubated for 1 hr at room temperature with anti-rabbit or anti-mouse Alexa Fluor 488- and/or 555-conjugated secondary antibodies (Life Technologies). Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole. The primary antibodies used were as follows: rabbit anti-OCT4 (Abcam, catalog no. ab19857), rabbit anti-SOX2 (Abcam, catalog no. ab97959), mouse anti-SSEA4 (Abcam, catalog no. ab16287), mouse anti-TRA-1-81 (Abcam, catalog no. ab16289), mouse anti-AFP (Abcam, catalog no. ab3980), rabbit anti-SMA (Abcam, catalog no. ab5694), mouse anti-NESTIN (Life Technologies, catalog no. A24353), goat anti-SOX1 (Life Technologies, catalog no. A24354), rabbit anti-SOX2 (Life Technologies, catalog no. A24354), rabbit anti-PAX6 (Life Technologies, catalog no. A24354), rabbit anti-β-III-TUBULIN (TUJ1; Abcam, catalog no. ab18207), and rabbit anti-GFAP (Abcam, catalog no. ab7260).

Li Y., Cao J., Chen M., Li J., Sun Y., Zhang Y., Zhu Y., Wang L, & Zhang C. (2017). Abnormal Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Partially Mimicked Development of TSC2 Neurological Abnormalities. Stem Cell Reports, 8(4), 883-893.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!