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Anti hsp70 spa810

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Anti-Hsp70 (SPA810) is a primary antibody that targets the heat shock protein 70 (Hsp70). Hsp70 is a highly conserved molecular chaperone involved in various cellular processes. The Anti-Hsp70 (SPA810) antibody can be used for the detection and quantification of Hsp70 in various applications, such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA).

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Lab products found in correlation

Dual luciferase kit, by Promega (2 mentions) Mitochondria gfp alexa fluor 488 or 546 labeled secondary antibodies donkey anti mouse igg h l, by Thermo Fisher Scientific (2 mentions) Anti mrps18b 16139 1 ap, by Proteintech (2 mentions) Anti rpl4 11302 1 ap, by Proteintech (2 mentions) Cleaved caspase 3 9661, by Cell Signaling Technology (2 mentions) Mitotracker, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Hoechst, by Thermo Fisher Scientific (2 mentions) β actin a5441, by Merck Group (2 mentions) Puromycin, by Merck Group (2 mentions)

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HSP70 (48 citations) Anti-HSP70 (15 citations)

2 protocols using anti hsp70 spa810

1

Regulation of Mitochondrial Ribosome Biogenesis

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HeLa cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% fetal bovine serum (FBS). HSF1+/+ and HSF1−/− MEFs were kindly provided by I. J. Benjamin (University of Utah). Cycloheximide (CHX), poly-U and puromycin were purchased from Sigma. MitoTracker, Mitochondria-GFP Alexa Fluor 488 or 546 labeled secondary antibodies [donkey anti-mouse IgG (H+L)] and Hoechst were purchased from Invitrogen. Anti-MRPL18 (HPA028774), anti-MRPL38 (HPA023054), anti-RPS20 (HPA003570), anti-RPL5 (HPA043717), anti-myc (C3956) and β-actin (A5441) monoclonal antibody was purchased from Sigma; anti-MRPS18B (16139-1-AP) and anti-RPL4 (11302-1-AP) was from Proteintech; anti-Hsp70 (SPA810) was from Enzo Life Sciences; anti-RPS6 (#2217), anti-HSP60 (#4870), anti-Caspase 3 (#9662)and cleaved Caspase 3 (#9661) antibody were from Cell Signaling. Dual luciferase kit was purchased from Promega. siRNA SMARTpools targeting human MRPL18 and control were purchased from Dharmacon. siRNA targeting human Lyn, MRPL38 and MRPS18B were purchase from Santa Cruz Biotechnology. Plasmids and siRNA transfections were performed using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instruction.
Zhang X., Gao X., Coots R.A., Conn C.S., Liu B, & Qian S.B. (2015). Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18. Nature structural & molecular biology, 22(5), 404-410.
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2

Regulation of Mitochondrial Ribosome Biogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% fetal bovine serum (FBS). HSF1+/+ and HSF1−/− MEFs were kindly provided by I. J. Benjamin (University of Utah). Cycloheximide (CHX), poly-U and puromycin were purchased from Sigma. MitoTracker, Mitochondria-GFP Alexa Fluor 488 or 546 labeled secondary antibodies [donkey anti-mouse IgG (H+L)] and Hoechst were purchased from Invitrogen. Anti-MRPL18 (HPA028774), anti-MRPL38 (HPA023054), anti-RPS20 (HPA003570), anti-RPL5 (HPA043717), anti-myc (C3956) and β-actin (A5441) monoclonal antibody was purchased from Sigma; anti-MRPS18B (16139-1-AP) and anti-RPL4 (11302-1-AP) was from Proteintech; anti-Hsp70 (SPA810) was from Enzo Life Sciences; anti-RPS6 (#2217), anti-HSP60 (#4870), anti-Caspase 3 (#9662)and cleaved Caspase 3 (#9661) antibody were from Cell Signaling. Dual luciferase kit was purchased from Promega. siRNA SMARTpools targeting human MRPL18 and control were purchased from Dharmacon. siRNA targeting human Lyn, MRPL38 and MRPS18B were purchase from Santa Cruz Biotechnology. Plasmids and siRNA transfections were performed using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instruction.
Zhang X., Gao X., Coots R.A., Conn C.S., Liu B, & Qian S.B. (2015). Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18. Nature structural & molecular biology, 22(5), 404-410.
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