129SvEv mice were purchased from Taconic. C57BL/6 mice and the congenic strain B6.SJL-Ptprca Pepcb/BoyJ (B6.SJL) were purchased from the Jackson Laboratory or NCI. Ho1−/− and miR-155−/− mice were purchased from the Jackson Laboratory. Nrf2−/− mice were kindly provided by Dr. Rajasekaran and Dr. Wang at the University of Utah. Mice were bred and maintained in our specific pathogen-free animal facility according to institutional guidelines.
B6 sjl ptprc a pepc b boyj b6 sjl
Manufactured by Jackson ImmunoResearch
Sourced in Montenegro
B6.SJL-Ptprc a Pepc b /BoyJ (B6.SJL) is a laboratory mouse strain that is widely used in immunological research. This strain is characterized by the expression of the Ptprc a allele, which encodes the CD45.1 isoform of the CD45 protein. This strain is often used as a tool to distinguish between different populations of immune cells in experimental settings.
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Lab products found in correlation
D mannose, by Merck Group (2 mentions)
B6 mrl fas lpr j b6 lpr mice, by Jackson ImmunoResearch (2 mentions)
C57bl 6j b6 mice, by Jackson ImmunoResearch (2 mentions)
Mir 155 mice, by Jackson ImmunoResearch (1 mentions)
129 svev mice, by Taconic Biosciences (1 mentions)
C57bl 6 mice, by Taconic Biosciences (1 mentions)
C57bl 6 wt, by Jackson ImmunoResearch (1 mentions)
C57bl 6 tg tcratcrb 1100mjb j ot 1, by Jackson ImmunoResearch (1 mentions)
5 protocols using b6 sjl ptprc a pepc b boyj b6 sjl
1
Mouse Strains Utilized for Research
2
Murine Experimental Models Acquisition
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WT 129SvEv mice were purchased from Taconic. WT C57BL/6, B6.SJL-PtprcaPepcb/BoyJ (B6.SJL), and C57BL/6-Tg(TcraTcrb)1100Mjb/J (OT-1) mice were purchased from The Jackson Laboratory. Mice designated as WT were from in-house breeding of C57BL/6 or 129SvEv strains. Experiments done with Rab43f/f mice used littermate controls. MHCI KO mice (Kb−/−Db−/−β2m−/−; TKO) were a gift from H.W. Virgin and T. Hansen (Washington University, St. Louis, MO; Lybarger et al., 2003 (link)). Experiments were performed with age- and sex-matched mice between 6 and 12 wk of age. All mice were bred and maintained in specific pathogen–free facilities according to institutional guideline protocols approved by the Animal Studies Committee of Washington University in St. Louis.
3
Generation of Conditional Dhx15 Knockout Mice
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Dhx15flox ES cell line in the C57Bl/6 background was obtained from EUCOMM, and injection of pseudo-pregnant females was performed by the Mouse Genetic Core at The Scripps Research Institute (La Jolla, CA). Briefly, a construct containing the Dhx15 exon2 sequence flanked by two flox sites was used for the generation of the targeted knock-in in JM8A3.N1 ES cell line. Neomycin resistant ES clones were selected and screened for locus-specific target insertion by PCR, and positive ES clones were selected for in vivo injections. Neomycin resistant gene was excised by crossing Dhx15flox mice to a B6.FLPo expressing strain. Dhx15flox/flox mice were maintained in house as either homozygous or crossed to Cd19cre and Cd4Cre. The B6.129P2(C)-Cd19tm1(cre)Cgn/J (Cd19cre) mouse was provided by Rickert et al. (18 (link)). B6.SJL-PtprcaPepcb/BoyJ (B6.SJL) and B6.Cg-Tg(Pgk1-flpo)10Sykr/J (B6.FLPo) (19 (link)) mice were purchased from Jackson Laboratory (Bar Harbor, ME). Validation of DHX15 knock-out is shown in Supplementary Figure 2 . All mice were backcrossed and housed in the Animal Facility at Sanford Burnham Prebys Medical Discovery Institute (La Jolla, CA), and experiments were conducted with the approval of the Institute's IACUC ethics committee.
4
Mannose Regulates Immune Response
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Mice C57BL/6J (B6) mice, B6(C)-H2-Ab1 bm12 /KhEgJ (bm12), B6.CgTg (TcraTcrb) 425Cbn/J (OT-II), B6.SJL-Ptprc a Pepc b /BoyJ (B6.SJL) and B6.MRL-Fas lpr /J (B6.lpr) mice were obtained from the Jackson Laboratory. All mice were bred and maintained at the University of Florida in speci c pathogen-free conditions. Both males and females were used between 2 and 6 months of age. Gender and age were matched for each experiment. D-mannose (Sigma) was dissolved at 1.1 M in drinking water and administered ad lib.
5
Mannose Regulates Immune Response
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Mice C57BL/6J (B6) mice, B6(C)-H2-Ab1 bm12 /KhEgJ (bm12), B6.CgTg (TcraTcrb) 425Cbn/J (OT-II), B6.SJL-Ptprc a Pepc b /BoyJ (B6.SJL) and B6.MRL-Fas lpr /J (B6.lpr) mice were obtained from the Jackson Laboratory. All mice were bred and maintained at the University of Florida in speci c pathogen-free conditions. Both males and females were used between 2 and 6 months of age. Gender and age were matched for each experiment. D-mannose (Sigma) was dissolved at 1.1 M in drinking water and administered ad lib.
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