Female mice of the c57Bl/6j strain (Janvier Labs, Paris) weighing 25–30 g were used in our study. A standard pellet diet and tap water were available ad libitum before and during the experiments. The experimental protocols approved by the local animal welfare committee, Lund, Sweden,
C57bl 6j strain
Manufactured by Janvier Labs
Sourced in France
The C57BL/6j strain is a widely used mouse model in biomedical research. It is an inbred mouse strain that has been selectively bred for genetic consistency. The C57BL/6j strain is commonly used as a control or background strain in various experimental studies.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using c57bl 6j strain
1
Female C57BL/6J Mice Study
2
Conditional Deletion of Cox-2 in Myeloid Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult wild-type mice of both sexes of the C57BL/6J strain were obtained from Janvier labs. Mice with selective deletion of Cox-2 in myeloid cells were generated by crossing mice possessing loxP sites flanking exons 4–5 of the Ptgs2 gene [18 (link)] with mice expressing Cre recombinase under the LysM promoter [19 (link)]. The mice were housed under constant ambient temperature (21°C), on a 12 h/12 h light/dark cycle (lights on at 7 A.M.) with food and water available ad libitum. All animal experiments were approved by the Animal Care and Use Committee at Linköping University (#1854/2018).
3
Controlled Conditions for C57BL/6j Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male mice (n = 53 per treatment group) of the C57BL/6j strain (Janvier Labs, Le Genest-Saint-Isle, France) bred in our laboratory were housed in a conventional facility after weaning under a controlled photoperiod (12:12h light dark cycle-lights on at 1 p.m.), maintained at 22°C and relative humidity of 60 ± 10%, with free access to water and a standard diet (A03-10; Safe-diets, Augy, France). The mice were housed in nest-enriched polysulfone cages, with polysulfone bottles. Offspring were mixed at weaning to avoid potential litter effects, with no more than one male per litter per cage, and were allowed to grow to 8 weeks of age.
4
Murine BBB Permeability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Males of C57BL/6j strain (Janvier Labs, Le Genest-Saint-Isle, France) bred in our laboratory were housed in a conventional facility after weaning under controlled photoperiod (12:12h light dark cycle-lights on at 1 p.m.), maintained at 22°C and relative humidity (60% ± 10%), and had free access to water and a standard diet (A03-10; Safe-diets, Augy, France). The mice were housed in nest-enriched polysulfone cages, with polysulfone bottles. Offspring were mixed at the weaning to avoid potential litter effects with no more than one male per litter per cage, and were allowed to grow to 8 weeks of age. For these experiments, 9 cohorts each comprising 9 to 32 animals distributed equally between the 4 treatment groups, were used. Ultimately, 32 animals from two cohorts were used for BBB permeability assays, 96 animals from five cohorts were used for the capillary-enriched fraction procedure and Western blot analysis, and then 68 animals from three cohorts were used for the immunocytochemistry study.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!