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Pre transformed human kidney matchmaker cdna library

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The Pre-Transformed Human Kidney Matchmaker cDNA Library is a collection of genetically modified human kidney cell-derived complementary DNA (cDNA) sequences. This library is designed to facilitate the identification and study of protein-protein interactions in the human kidney.

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Matchmaker cDNA library (17 citations) Human kidney cDNA library (6 citations) Human cDNAs (2 citations)

2 protocols using pre transformed human kidney matchmaker cdna library

1

Yeast Two-Hybrid Screening for PRDX6 Interactors

Cited 1 time
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AE1(C)WT (residues 876-911) or AE1(C)Δ11 (residues 876-900) were used as bait protein to screen the Pre-Transformed Human Kidney Matchmaker cDNA Library (Clontech) as previously described.13 (link) Confirmation of potential interaction with PRDX6 was assessed by mating back to the original bait of either AE1(C)WT, AE1(C)Δ11 or a control vector (p53). Confirmed clones were sequenced and identified through BLAST searches (blast.ncbi.nlm.nih.gov).
Sorrell S.L., Golder Z.J., Johnstone D.B, & Frankl F.E. (2016). Renal peroxiredoxin 6 interacts with anion exchanger 1 and plays a novel role in pH homeostasis. Kidney international, 89(1), 105-112.
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2

Yeast Two-Hybrid Screening for PRDX6 Interactors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
AE1(C)WT (residues 876-911) or AE1(C)Δ11 (residues 876-900) were used as bait protein to screen the Pre-Transformed Human Kidney Matchmaker cDNA Library (Clontech) as previously described.13 (link) Confirmation of potential interaction with PRDX6 was assessed by mating back to the original bait of either AE1(C)WT, AE1(C)Δ11 or a control vector (p53). Confirmed clones were sequenced and identified through BLAST searches (blast.ncbi.nlm.nih.gov).
Sorrell S.L., Golder Z.J., Johnstone D.B, & Frankl F.E. (2016). Renal peroxiredoxin 6 interacts with anion exchanger 1 and plays a novel role in pH homeostasis. Kidney international, 89(1), 105-112.
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+ Expand

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