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Cd4 fitc rm4 4

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The CD4 FITC (RM4-4) is a fluorescently labeled antibody that binds to the CD4 receptor on the surface of T helper cells. It is a research-use-only product designed for flow cytometry applications.

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Lab products found in correlation

Anti cd45.2 fitc, by BD (2 mentions) Anti cd45.2 apc, by BioLegend (2 mentions) Anti cd90.2 apc, by BioLegend (2 mentions) Cd40 apc, by BioLegend (1 mentions) Percp streptavidin, by BioLegend (1 mentions) Cd11b fitc, by BioLegend (1 mentions) Cd206 fitc, by BioLegend (1 mentions) Ly6c fitc, by BioLegend (1 mentions) Fix perm kit, by Thermo Fisher Scientific (1 mentions) B220 apc ra3 6b2, by BioLegend (1 mentions) Accuri c6 cytometer, by BD (1 mentions) Cd11c pe hl 3, by BD (1 mentions) F4 80 fitc bm8, by BioLegend (1 mentions) Ccr2 fitc, by BioLegend (1 mentions) Ter119 biotin ter 119, by BioLegend (1 mentions) Cd107b fitc, by BioLegend (1 mentions)

Spelling variants of this product

CD4-FITC (139 citations) RM4-4 (10 citations) CD4-FITC (clone RM4-4; (2 citations)

3 protocols using cd4 fitc rm4 4

1

Comprehensive Immune Cell Profiling by Flow Cytometry

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Mononuclear cells were stained with fluorescently labeled antibodies to CD11c-PE (HL3, BD Biosciences), CD4-FITC (RM4-4, Biolegend, San Diego, CA, USA), B220-biotin (RA3-6B2, Biolegend), B220-APC (RA3-6B2, Biolegend), I-A/I-E-APC (MHC class II, M5/114.15.2, Biolegend), CD40-APC (3/23, Biolegend), CD80-FITC (16-10A1, Biolegend), CD86-FITC (GL-1, Biolegend), CD3ε-biotin (145-2C11), Gr-1-biotin (RB6-8C5, Biolegend), TER119-biotin (TER-119, Biolegend), F4/80-FITC (BM8, Biolegend), CD11b-FITC (M1/70, Biolegend), CD107b-FITC (M3/84, Biolegend), FcεR1α-FITC (MAR-1, Biolegend), CD206-FITC (C068C2, Biolegend), Ly6c-FITC (HK1.4, Biolegend), CCR2-FITC (SA203G11, Biolegend), and CCR7-AlexaFluor488 (4B12, Biolegend). PerCP Streptavidin (Biolegend) was also used to stain the cells treated with biotinylated mAbs. For intracellular staining experiment, FIX & PERM Kit (Thermo Fisher Scientific) was used to stain CD4 T cells with mAb to FoxP3-PE (MF-14, Biolegend). After washing twice with buffer (PBS buffer containing 2% FBS, 2 mM EDTA, and 0.02% sodium azide), the expression level was analyzed by flow cytometry using a BD Accuri C6 cytometer (BD Biosciences). Data analysis was done by using BD Accuri C6 software.
Darkwah S., Nago N., Appiah M.G., Myint P.K., Kawamoto E., Shimaoka M, & Park E.J. (2019). Differential Roles of Dendritic Cells in Expanding CD4 T Cells in Sepsis. Biomedicines, 7(3), 52.
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2

Lung Cell Trafficking Discrimination

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Anti-CD45.2 FITC (BD Biosciences), 2.5 μg/mouse was given intravenously 3 min. prior to lung harvest to distinguished parenchymal-localized (IV−) and vasculature-associated (IV+) cells in CB6F1 mice. In C57BL/6 mice, anti-CD90.2 APC (BioLegend, San Diego, CA, USA), anti-CD45.2 APC (BioLegend) or CD4 FITC (RM4-4; BioLegend) was injected intravenously at 1 μg/mouse, 1 μg/mouse, and 2.5 μg/mouse, respectively, 10 min prior to lung harvest.
Woodworth J.S., Cohen S.B., Moguche A.O., Plumlee C.R., Agger E.M., Urdahl K.B, & Andersen P. (2016). Subunit vaccine H56/CAF01 induces a population of circulating CD4 T cells that traffic into the Mycobacterium tuberculosis-infected lung. Mucosal immunology, 10(2), 555-564.
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3

Lung Cell Trafficking Discrimination

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Anti-CD45.2 FITC (BD Biosciences), 2.5 μg/mouse was given intravenously 3 min. prior to lung harvest to distinguished parenchymal-localized (IV−) and vasculature-associated (IV+) cells in CB6F1 mice. In C57BL/6 mice, anti-CD90.2 APC (BioLegend, San Diego, CA, USA), anti-CD45.2 APC (BioLegend) or CD4 FITC (RM4-4; BioLegend) was injected intravenously at 1 μg/mouse, 1 μg/mouse, and 2.5 μg/mouse, respectively, 10 min prior to lung harvest.
Woodworth J.S., Cohen S.B., Moguche A.O., Plumlee C.R., Agger E.M., Urdahl K.B, & Andersen P. (2016). Subunit vaccine H56/CAF01 induces a population of circulating CD4 T cells that traffic into the Mycobacterium tuberculosis-infected lung. Mucosal immunology, 10(2), 555-564.
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