Mouse anti rabbit igg antibody

Zoledronate, galangin, icariside II, kaempferol, quercetin, Zoledronate, galangin, icariside II, kaempferol, quercetin (Figure 1), and 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyl tetrazolium bromide reagents were purchased from Sigma (St. Louis, MO), and 3‐methyladenine (3‐MA; a type‐III phosphatidylinositol 3‐kinase [PI‐3 K] inhibitor) and rapamycin reagents were obtained from Calbiochem (La Jolla, CA). The antibodies against beclin‐1 and Autophagy protein 5 (ATG5) were purchased from Cell Signaling Technology (Beverly, MA). The antibodies against collagen I, osteocalcin, bone morphogenetic protein 2 (BMP‐2), Osterix, and RUNX2 were purchased from ABCam (Cambridge, MA). The antibodies against LC3 were purchased from Sigma. SQSTM1/p62, β‐actin antibody, mouse anti‐rabbit IgG antibody, and rabbit anti‐mouse IgG antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). All other chemicals and reagents were purchased from Sigma unless otherwise specified.

Alpha-MEM, EGF, bFGF, propidium iodide (PI), and RNAase A were purchased from Invitrogen (Hong Kong, China). HOE140, 5-Bromo-2′-deoxyuridine (BrdU), and mouse monoclonal anti-BrdU antibody were from Sigma-Aldrich (St. Louis, MO USA). Rabbit polyclonal anti-Akt, anti-pAkt(Ser473), anti-ERK1/2 and anti-pERK1/2 antibodies were from Cell Signaling (Danvers, MA, USA). Mouse monoclonal anti-BDKRB2 antibody was from Millipore (Merck Millipore, Germany). B2R siRNA, mouse monoclonal anti-cyclin D1, mouse monoclonal anti-cyclin E, HRP-conjugated goat anti-rabbit or rabbit anti-mouse IgG antibody were products of Santa-Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Transwell permeable support polycarbonate membrane and other cell culture flasks and plates were purchased from Corning Inc. (NY, USA).

The following reagents were obtained commercially:
3‐[4,5‐dimethylthiazol‐2‐yl]2,5‐diphenyl tetrazolium bromide (MTT),
monodansylcadaverine (MDC), acridine orange were purchased from Sigma (St. Louis,
Missouri). 3‐Methyladenine (3‐MA, class III PI3K inhibitor) was obtained from
Calbiochem (La Jolla, California). Antibodies against the cleaved form of caspase‐3,
caspase‐8, Beclin‐1, and PARP were purchased from Cell Signaling Technology (Beverly,
MA). Antibodies against LC3 (Sigma) were also used. The p62/SQSTM1, caspase‐9,
ATG5‐ATG12 complex, GAPDH, mouse antiactin antibody, mouse antirabbit IgG antibody,
and rabbit antimouse IgG antibodies were purchased from Santa Cruz Biotechnology
(Santa Cruz, California). All other chemicals and reagents were purchased from Sigma
unless otherwise specified.