We recorded baseline characteristics including age, sex, time since onset of Chikungunya symptoms, as well as presence or absence of the following features: fever, arthralgia (any of the following: knee, ankle, metacarpo-phalangeal joints, wrist, elbow, shoulder girdle, and pelvis), myalgia, digestive or neurological symptoms, and comorbidity burden (assessed using Charlson’s comorbidity index [17 (link)]). The Charlson’s comorbidity index measures patient comorbidity using the tenth International Classification of Diseases Diagnoses Codes. Each comorbidity has a weight (from 1 to 6) depending on its severity. The higher the score, the higher is the comorbidity burden. Biological testing included: white cells, neutrophils, lymphocytes, and RT-PCR. All patients included in this study had serum samples tested using RT-PCR with the RealStar® Chikungunya RT-PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany). We considered as confirmed CVI all suspected cases in whom biological confirmation was obtained by positive RT-PCR. The Mayotte tool and Reunion Island tool were calculated for all patients.
Realstar chikungunya rt pcr kit
Manufactured by Altona Diagnostics
Sourced in Germany
The RealStar® Chikungunya RT-PCR Kit is a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay designed for the detection of Chikungunya virus RNA.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using realstar chikungunya rt pcr kit
1
Chikungunya Virus Infection Baseline Characteristics
2
CHIKV and DENV Detection Assay Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Culture supernatants of CHIKV‐Asian and DENV 1 to 4 were serially diluted 10‐fold in EDTA‐plasma. Each concentration was tested in three replicates on cobas CHIKV/DENV. Each concentration was also tested in six replicates by the first generation RealStar Chikungunya RT‐PCR kit27 or second generation RealStar Dengue RT‐PCR kit 28 (Altona Diagnostics).
For the RealStar assays, three extractions were performed on each viral concentration using the QIAamp Viral RNA Mini Kit (QIAGEN), with sample input volume doubled from 140 to 280 μL to increase sensitivity. From each extraction 60 μL was eluted and two replicates of RealStar RT‐PCR testing were performed from each eluate using 10 μL of eluate per PCR, for a total of six PCR results for each viral concentration.
For the RealStar assays, three extractions were performed on each viral concentration using the QIAamp Viral RNA Mini Kit (QIAGEN), with sample input volume doubled from 140 to 280 μL to increase sensitivity. From each extraction 60 μL was eluted and two replicates of RealStar RT‐PCR testing were performed from each eluate using 10 μL of eluate per PCR, for a total of six PCR results for each viral concentration.
3
Chikungunya Virus Clinical Characteristics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We recorded baseline characteristics including age, sex, and presence or absence of the following features: fever, arthralgia (any of the following: knee, ankle, metacarpo-phalangeal joints, wrist, elbow, shoulder girdle, and pelvis), low back pain, myalgia, digestive symptoms (nausea, vomiting, diarrhoea…) or neurological symptoms (headaches, space-time disorientation, seizures, neurological deficit, unconsciousness…), and comorbidity burden, assessed using Charlson’s comorbidity index. Biological testing included neutrophils and lymphocyte count, and RT-PCR. Lymphopenia was defined as a count below 1000 lymphocytes per microliter. Neutrophil leukocytosis was considered to be absent if the neutrophil count was below 7500 per microliter. In the acute phase, RT-PCR is considered as the gold standard to identify subjects with or without CHIKV. All patients included in this study underwent serum sample testing using RT-PCR with the RealStar® Chikungunya RT-PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany).
4
Retrospective Analysis of Chikungunya Virus Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Data were retrospectively collected from medical files. The following baseline characteristics were recorded: age, sex, presence or absence of fever and/or polyarthralgia, and hospital stay. We also recorded any of the following unusual concurrent clinical manifestations at admission: infectious disease (other than Chikungunya virus infection), neurological troubles (including confusion or delirium, seizure, syncope, encephalitis, and sensorimotor deficit), cardiovascular disorders (including rhythm disorder, cardiac decompensation, and myocardial ischemia), decompensated diabetes (diabetes with negative impact on glycemic control), respiratory disorders (including dyspnea, respiratory infection based on clinical examination, or positive sputum gram stain, or culture for bacteria, or simply an infiltrate on chest X-Ray), renal failure, hepatic disorders, lymphopenia (count <1000/mm3), absence of neutrophilia (count <7500/mm3), thrombocytopenia (count <100,000/μL), hypernatremia (blood sodium level >145mmol/L). The comorbidity burden was assessed using Charlson’s comorbidity index.11 (link) All subjects included in this study underwent serum sample testing using RT-PCR with the RealStar® Chikungunya RT-PCR Kit (Altona Diagnostics GmbH, Hamburg, Germany), and were classified as Chik+ (for positive RT-PCR) and Chik- (for negative RT-PCR).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!