In order to observe the internal microstructure via a scanning electron microscope (SEM) (Model Evo18 Carl Zeiss, Oberkochen, Germany) and an environmental scanning electron microscope (ESEM-FEG) (Model XL-30, FEI Company, Oregon, OR, USA), the corresponding samples were placed in liquid nitrogen. After freeze-drying in a freeze drying oven (LGJ-10C, Beijing Four Ring Scientific Instrument Factory Co., Ltd., Beijing, China) to remove water thoroughly, the hydrogels were sputtered with gold and observed.
Model evo 18
Manufactured by Zeiss
Sourced in Germany
The Zeiss Model EVO-18 is a scanning electron microscope (SEM) that provides high-resolution imaging of samples. It is equipped with a tungsten filament electron source and features automated control and data acquisition capabilities.
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Lab products found in correlation
7 protocols using model evo 18
1
Hydrogel Microstructure Analysis via SEM
2
Microscopic Analysis of Beetle Hind Wings
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A super depth-of-field microscope (VHX-6000, Keyence, Japan) was used to obtain images of the fully unfolded and folded hind wings of the three beetles. To obtain the macroscopic structures of the hind wings of the three beetles, the hind wings were first removed with a scalpel and rinsed with distilled water and then dried and pasted flat on a slide for observation.
Scanning electron microscopy (SEM) (Model EVO-18, Carl Zeiss Microimaging Inc., Germany) was used to obtain morphological images of cross sections of the hind wings of three beetles at the same locations of different wing veins.
Scanning electron microscopy (SEM) (Model EVO-18, Carl Zeiss Microimaging Inc., Germany) was used to obtain morphological images of cross sections of the hind wings of three beetles at the same locations of different wing veins.
3
Sorghum and Reed Tissue Analysis via SEM-EDS
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Microscopic research was carried out using a Zeiss scanning electron microscope (SEM, ModelEVO-18, Jena, Germany). The main parameters of SEM were an experimental magnification range of 13–50,000 times magnification, and a minimum resolution of 3.0 nm. Additionally, an energy-dispersive spectrometer (EDS) with a resolution ratio of 6 nm provided the chemical composition of fur samples inside SEM. The elemental composition and contents of the fiber bundles of sorghum, basic tissues of sorghum, mechanical tissues of reed and thin-walled tissues of reed were measured using an energy-dispersive spectrometer (EDS, JSM-5301, Munich, Germany) equipped with SEM.
4
Nano-silver Effects on Bacterial Ultrastructure
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Samples from bacterial cultures (Bacillus megaterium MTCC 7192 and Pseudomonas aeruginosa MTCC 741), mixed with 100 μg/ml of nano-silver, were collected at 3 h and pre-fixed with 2.5% glutaraldehyde for 30 min; these were then washed two times in the same buffer and post-fixed for 2 h in 1% osmium tetroxide. After washing with buffer, dehydration process was conducted with 30, 50, 70, 80, 90 and 100% of ethanol. Prior to analysis by SEM (Zeiss, Model EVO-18), the samples were dried at 40 °C and subjected to analysis with 15 kV accelerating voltage. Control experiment was conducted in absence of nano-silver [16 (link)].
5
Ultrasonication Morphological Analysis by SEM
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The morphological changes brought by ultrasonication were analyzed using SEM (ZEISS EVO18 MODEL). The samples were mounted on a stub using a double sticky tape and coated with the thick film of gold. The analysis was carried under low vacuum at an accelerating voltage of 20 KV.
6
Comprehensive Characterization of Nanodiamond
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The Structural morphology of the samples was determined by scanning electron microscopy (SEM, EVO 18 model from Carl Zeiss, Germany, Filament: Tungsten) and High-Resolution Transmission Electron Microscopy (HRTEM, 80 kV) from JEOL (JEM 2100, Japan). Selected area diffraction pattern (SAED) of ND was recorded using HRTEM. Other characterization techniques used in this study were XRD (Empyrean Malvern Panalytical diffractometer with MultiCore Optics, Cu Kα, λ = 1.54 Å), Fourier Transform Infra-Red Spectroscopy (FTIR 400–4000 cm−1 range, IR affinity series, Shimadzu, Japan), Particle Size Analyzer with zeta potential (Malvern Panalytical, Zetasizer Ver. 7.13), Rheometer (Anton Paar/Modular Compact Rheometer MCR 102, 37 ºC, Gap-1.025 mm, Measuring System- pp25) and Universal testing machine for compression test (INSTRON 3366, Speed-1 mm/min; dimensions of the cylindrical samples: 16 ± 0.1 mm diameter, 13 ± 0.2 mm height). ND (0.04 g) was dispersed in water with vortex shaking for 1 h and ultrasonic treatment of 15 min for particle size and zeta potential analysis. Dynamic Mechanical analysis (DMA) was carried out in the rheometer instrument in Torsional Mode (1% shear strain at 37 °C, frequency 1 – 10 Hz, cuboid samples: 10 mm × 5 mm × 3 mm).
7
Characterization of Optimized TAC-THQ-NLCs
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The shape and surface morphology of the optimized TAC-THQ-NLCs were characterized with the help of an electron microscope utilizing an instrument, transmission electron microscope (TEM), and scanning electron microscopy (SEM). For TEM analysis, one drop of 20 times diluted optimized formulation was mounted on a copper grid coated with copper. Thereafter, the formulation-bearing grid was stained negatively with phosphotungstic acid (2% w/v) to enhance the contrast. Finally, the sample was air-dried at room temperature before being examined under TEM (HR-TEM, Fei, Electron Optics, Netherlands) operated at 200 kV with point-to-point resolution. For SEM analysis, 20 µL of the formulation was placed on the aluminum stub-bearing double-sided tape. Thereafter, the formulation-bearing stub was subjected to sputter coating with a mixture of platinum and palladium until a thickness of 5 nm was achieved to improve overall conductivity. Finally, the sample was visualized under SEM (SEM, EVO 18 model, Zeiss, Germany) operated at 20 kV voltage.
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