α-32P dCTP (6,000 Ci/mmol) was purchased from PerkinElmer (Boston, MA). Antibodies used for Western, ChIP, Seq-ChIP, and immunohistochemistry (IHC) to PHB1, MATα1, c-MYC, MAFG, c-MAF, β-ACTIN, and IgG were purchased from Abcam (Cambridge, MA). Lipofectamine 2000 and RNAi-Max were purchased from ThermoFisher (Carlsbad, CA). siRNAs to PHB1 (Cat# 4392422), c-MYC (5′-CGAUUCCUUCUAACAGAAtt-3′), MAFG (5′-CGGACUAGAGAGAGUUGCGtt-3′), c-MAF (5′-GCAUCGUGUACUUACCAGUtt-3′) and MAT1A (5′-GCACAACGAAGACAUCACGtt-3′) were purchased from ThermoFisher. Bile acids and interleukin-6 (IL-6) were from Sigma (St. Louis, MS).
Interleukin 6 il 6
Manufactured by Merck Group
Sourced in United States
Interleukin-6 (IL-6) is a cytokine that plays a crucial role in the immune response and inflammation. It is a protein secreted by various cell types, including T cells, B cells, and macrophages. IL-6 is involved in the regulation of the acute phase response, the stimulation of immune cell growth and differentiation, and the modulation of inflammatory processes.
Automatically generated - may contain errors
Lab products found in correlation
Stat3, by Cell Signaling Technology (2 mentions)
Rnaimax, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Cycloheximide (chx), by Merck Group (2 mentions)
Bile acid, by Merck Group (1 mentions)
C maf, by Abcam (1 mentions)
C myc, by Thermo Fisher Scientific (1 mentions)
Seq chip, by Abcam (1 mentions)
Matα1, by Abcam (1 mentions)
α 32p dctp, by PerkinElmer (1 mentions)
β actin, by Abcam (1 mentions)
C myc, by Abcam (1 mentions)
Blood insulin, by R&D Systems (1 mentions)
Tnf α, by Merck Group (1 mentions)
Aspirin, by Merck Group (1 mentions)
Phosphor mtor, by Cell Signaling Technology (1 mentions)
Cox 2, by Cell Signaling Technology (1 mentions)
Phosphor acc, by Cell Signaling Technology (1 mentions)
Phosphor 4e bp1, by Cell Signaling Technology (1 mentions)
Phosphor ampkα, by Cell Signaling Technology (1 mentions)
6 protocols using interleukin 6 il 6
1
Western Blot and ChIP-Seq Workflow
2
Metabolic and Inflammatory Biomarkers
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Fasting blood glucose, total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-c) and low density lipoprotein cholesterol (LDL-c) were determined with a Hitachi 7100 auto-biochemical analyzer (Hitachi-Aloka Medical, Tokyo, Japan). Blood insulin (R&D Systems, Abnova, USA), tumor necrosis factor-α (TNF-α) (Sigma-Aldrich.com , St. Louis, MO, USA), and interleukin-6 (IL-6) (Sigma-Aldrich.com , St. Louis, MO, USA) were measured by enzyme linked immunosorbent assay methods.
3
Immunoblotting for Signaling Proteins
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Antibodies against phosphor-AMPKα, AMPKα, phosphor-ACC, ACC, phosphor-mTOR, mTOR, phosphor-p70S6K, p70S6K, phosphor-4E-BP1, 4E-BP1, phorphor-NFκB p65, NFκB p65, IκBα, COX2, phosphor-STAT3, STAT3, Mcl-1, Bcl-xL, Bim, Bak, Bax, Bid, Puma and Bad were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against Bcl-2 and Protein A/G Agarose beads were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Metformin and aspirin as well as interleukin-6 (IL-6) were purchased from Sigma-Aldrich (St Louis, MO, USA). AZD-8055 was purchase from Invitrogen (Carlsbad, CA, USA).
4
Molecular Mechanisms of Theaflavins in Liver Cancer
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HCCLM3, Huh-7, and LO2 cell lines were purchased from the Cell Resource Center of Shanghai Institute for Biological Sciences, Chinese Academy of Sciences (Shanghai, People’s Republic of China). The cells used in this paper were approved by the Committee of the Fifth Central Hospital of Tianjin. All the cells were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum. Theaflavins, interleukin-6 (IL-6), and benzyloxycarbonyl-valinyl-alaninyl-aspartyl fluoromethyl ketone were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). NSC 74859 was obtained from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Primary antibodies against Bcl-2, Survivin, cleaved-caspase-3, cleaved-caspase-9, cleaved-poly (ADP-ribose) polymerase (PARP), pro-caspase-3, pro-PARP, p-ERK, ERK, p-AKT, AKT, p-STAT3, and STAT3 were purchased from Cell Signaling Technology (Danvers, MA, USA). Glyceraldehyde 3-phosphate dehydrogenase and secondary antibodies against mouse IgG-horseradish peroxidase (HRP) and rabbit IgG-HRP were obtained from Santa Cruz Biotechnology. Antibodies against Ki-67, MMP-9, and MMP-2 were obtained from Abcam (Cambridge, UK).
5
Homoharringtonine and Docetaxel Cytotoxicity
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Homoharringtonine (HHT) with a purity of ≥98% was purchased from Aladdin Industrial Inc. (Shanghai, PR China). HHT was dissolved in phosphate buffered salineat (PBS) at a stock solution of 2.5 mM and kept at −20 °C. Docetaxel was provided by Beijing InnoChem Science & Technology Co., Ltd (Beijing, PR China). The 3-(4,5-dimethylthiahiazozy1)-3,5-di-phenytetrazoliumromide (MTT), Trypan Blue and Hoechst 33258 were purchased from Sigma-Aldrich. Interleukin 6 (IL-6) with a purity of ≥98% was purchased from EMD Millipore and dissolved in PBS at a stock solution of 100 μg/mL and stored at −20 °C. Cycloheximide (CHX) with a purity of ≥98% was also purchased from EMD Millipore and dissolved in ethanol at a stock solution of 10 mg/mL and stored at −20 °C. The JC-1 Apoptosis Detection Kit was obtained from KeyGEN Biotech Co., Ltd. (Nanjing, China). All other chemicals of the highest quality were commercially available and used as received.
6
Synthesis and Characterization of Tambjamine Analogue T21
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Tambjamine-21 analogue (T21) was synthesized as previously reported [6 (link)], dissolved at 10 mmol/L in dimethyl sulfoxide (DMSO) and stored at −20 °C. Cycloheximide (CHX; Cat#C7698-IG) from Sigma-Aldrich was dissolved in ethanol at a stock solution of 100 mg/mL and stored at −20 °C. Interleukin 6 (IL-6; Cat#IL006) was purchased from EMD Millipore and dissolved in 1× phosphate-buffered saline (PBS; Cat#02-020, Biological Industries, Beit Haemek, Israel) with calcium and magnesium supplemented with 0.1% bovine serum albumin (BSA; Cat#A7906, Sigma-Aldrich, St Louis MO, USA) at a stock solution of 100 µg/mL and stored at −80 °C. Hoechst 33,342 (Cat# B2261) was purchased from Sigma-Aldrich.
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