Log In Sign up
The largest database of trusted experimental protocols

Cd27 pe cy7 clone o323

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

CD27 PE-Cy7 (clone O323) is a fluorochrome-conjugated antibody that binds to the CD27 cell surface antigen. CD27 is a member of the tumor necrosis factor receptor family and is expressed on T cells, B cells, and natural killer cells. This antibody can be used for the detection and analysis of CD27-expressing cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Aqua vital dye, by Thermo Fisher Scientific (1 mentions) Igd pe clone ia6 2, by BD (1 mentions) Cd3 pe cy5 clone hit3a, by BD (1 mentions) Facsaria 2, by BD (1 mentions) Cd14 buv395 clone m5e2, by BD (1 mentions)

2 protocols using cd27 pe cy7 clone o323

1

ZIKV-Reactive B Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thawed PBMCs were stained with Aqua Vital Dye (Invitrogen), IgD-PE (clone IA6-2; BD Biosciences), CD10 PE-CF594 (clone HI10a; BD Biosciences; CD3 PE-Cy5 (clone HIT3a; BD Biosciences), CD14 BV605 (clone M5E2; BD Biolegend), CD16 BV570 (clone 3G8; Biolegend), CD27 PE-Cy7 (clone O323; Thermo Fisher Scientific), CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1; BD Biosciences) and 1 x 106 PFU of freshly thawed UV-inactivated ZIKV labelled with AF488. Additionally, 5 μM of Chk2 kinase inhibitor II (Calbiochem/EMD Chemicals) was added to prevent cell death. Unfractionated B cells were defined as CD14−/CD16−/CD3−/CD19+. For memory B cells an additional IgD−/CD27all gate was applied. The ZIKV-reactive AF488 gate was set using a fluorescence minus one condition. Cells were sorted on a Beckton Dickinson FACS Aria II and analysis was performed in FlowJo.
Singh T., Hwang K.K., Miller A.S., Jones R.L., Lopez C.A., Dulson S.J., Giuberti C., Gladden M.A., Miller I., Webster H.S., Eudailey J.A., Luo K., Von Holle T., Edwards R.J., Valencia S., Burgomaster K.E., Zhang S., Mangold J.F., Tu J.J., Dennis M., Alam S.M., Premkumar L., Dietze R., Pierson T.C., Eong Ooi E., Lazear H.M., Kuhn R.J., Permar S.R, & Bonsignori M. (2022). A Zika virus-specific IgM elicited in pregnancy exhibits ultrapotent neutralization. Cell, 185(25), 4826-4840.e17.
+ Open protocol
+ Expand
2

Quantifying S100A8/A9 Expression in SLE

Check if the same lab product or an alternative is used in the 5 most similar protocols
To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), CD3-BV421 (clone OKT3, BD Biosciences, Franklin Lakes, NJ, USA), CD14-BUV395 (clone M5E2, BD Biosciences, Franklin Lakes, NJ, USA), CD11c-V450 (clone B-ly6, BD Biosciences, Franklin Lakes, NJ, USA), CXCR5-APC (clone #51,505, R&D Systems, NE Minneapolis, MN, USA), CD27-PE-Cy7 (clone O323, Thermo Fisher Scientific, Waltham, MA, USA), IgD-Horizon V500 (clone IA6-2, BD Biosciences, Franklin Lakes, NJ, USA), S100A8/A9-FITC, and S100A8-FITC and analyzed using BD LSRFortessa™. Antibodies specific to the S100A8/A9 complex (clone 27E10, BMA Biomedicals, Rheinstrasse, Switzerland) and S100A8 (clone 3H2617, Life Span BioSciences, Huissen, Netherlands) were used. The relative expression levels of each molecule were compared by mean fluorescence intensity (MFI).
Kitagori K., Oku T., Wakabayashi M., Nakajima T., Nakashima R., Murakami K., Hirayama Y., Ishihama Y., Ohmura K., Morinobu A., Mimori T, & Yoshifuji H. (2023). Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus. Arthritis Research & Therapy, 25, 76.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!