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Ccl11

Manufactured by Thermo Fisher Scientific
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Sourced in France, United States

The CCL11 is a laboratory equipment product designed for specific applications. It serves a core function without further interpretation or extrapolation of its intended use.

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Lab products found in correlation

Cxcl12, by Thermo Fisher Scientific (3 mentions) Vascular endothelial growth factor (vegf), by Thermo Fisher Scientific (2 mentions) Ccl17, by Thermo Fisher Scientific (2 mentions) Ccl20, by Thermo Fisher Scientific (2 mentions) Cxcl8, by Thermo Fisher Scientific (2 mentions) Cxcl11, by Thermo Fisher Scientific (2 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (2 mentions) Cxcl9, by R&D Systems (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions) Cxcl1, by Thermo Fisher Scientific (1 mentions) Il 33, by R&D Systems (1 mentions) Il 10, by BioLegend (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Tnf α, by R&D Systems (1 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions) Ccl22, by Thermo Fisher Scientific (1 mentions) Ccl21, by Thermo Fisher Scientific (1 mentions) Cell culture media and supplements, by Thermo Fisher Scientific (1 mentions) Jetprime, by Polyplus Transfection (1 mentions)

7 protocols using ccl11

1

Cytokine Profiling in Murine Lung Fluids

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Pleural wash (diluted 1:4) or bronchoalveolar (diluted 1:4) fluids collected from individual mice were assayed for cytokine content by enzyme-linked immunosorbent assay (ELISA) in duplicate. These assays were performed according to the manufacturers’ recommendations, using the following kits, IFN-γ, CCL2, IL-4, IL-6 (eBiosciences SAS, France), CCL11 (Peprotech, France) and CXCL9 (R&D, UK). Results are shown as pg/mL. Detection limits were 4 pg/ml for IL-4 and IL-6, 15 pg/ml for INF-γ, CCL2 and CXCL9 and 30 pg/ml for CCL11.
Fercoq F., Remion E., Frohberger S.J., Vallarino-Lhermitte N., Hoerauf A., Le Quesne J., Landmann F., Hübner M.P., Carlin L.M, & Martin C. (2019). IL-4 receptor dependent expansion of lung CD169+ macrophages in microfilaria-driven inflammation. PLoS Neglected Tropical Diseases, 13(8), e0007691.
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2

Cytokine and Chemokine Quantification

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Cytokines and sCD300b were measured by ELISA according to manufacturer’s instructions using kits: IL-6, TNF-α, and IL-33 (R&D Systems, Minneapolis, MN), IL-10 (Biolegend San Diego, CA), CCL2, CXCL1, CCL11, EGF, and VEGF (peprotech, Rehovot, IL), sCD300b (OriGene Technologies Inc., Rockville, MD).
Avlas S., Kassis H., Itan M., Reichman H., Dolitzky A., Hazut I., Grisaru-Tal S., Gordon Y., Tsarfaty I., Karo-Atar D., Rozenberg P., Bitton A, & Munitz A. (2023). CD300b regulates intestinal inflammation and promotes repair in colitis. Frontiers in Immunology, 14, 1050245.
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3

Cytokine Ligand Procurement Protocol

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CCL2, CCL5, CCL11, CCL17, CCL20, CCL21, CCL22, CXCL8, CXCL11 and CXCL12 were purchased from PeproTech.
Zhang Y., Wu Y., Qi H., Xiao J., Gong H., Zhang Y., Xu E., Li S., Ma D., Wang Y., Li W, & Shen H. (2017). A new antagonist for CCR4 attenuates allergic lung inflammation in a mouse model of asthma. Scientific Reports, 7, 15038.
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4

Cell Culture Media Optimization

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Cell culture media and supplements were purchased from Life Technologies Invitrogen and Sigma. JetPrime® was from Polyplus. CXCL12 and CCL11 were purchased from Peprotech.
Lessel D., Muhammad T., Casar Tena T., Moepps B., Burkhalter M.D., Hitz M.P., Toka O., Rentzsch A., Schubert S., Schalinski A., Bauer U.M., Kubisch C., Ware S.M, & Philipp M. (2016). The analysis of heterotaxy patients reveals new loss-of-function variants of GRK5. Scientific Reports, 6, 33231.
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5

Reconstitution of Chemokine Ligands

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The human chemokines CCL1, CCL5, CCL7, CCL11, CCL17, CCL19, CCL20, CCL25, CCL27, CXCL8, CXCL11, CXCL12, CXCL13, CXCL16, and CX3CL1 were purchased from PeproTech, whereas XCL1 was purchased from R&D Systems. All chemokines were reconstituted in a buffer containing 0.1% (w/v) bovine serum albumin (BSA) and 1 mM acetic acid.
Berg C., Wedemeyer M.J., Melynis M., Schlimgen R.R., Hansen L.H., Våbenø J., Peterson F.C., Volkman B.F., Rosenkilde M.M, & Lüttichau H.R. (2022). The non-ELR CXC chemokine encoded by human cytomegalovirus UL146 genotype 5 contains a C-terminal β-hairpin and induces neutrophil migration as a selective CXCR2 agonist. PLoS Pathogens, 18(3), e1010355.
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6

Cytokine and Chemokine Profiling in BALF

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The levels of cytokines, including IL-4 (PeproTech, Rocky Hill, NJ, USA), IL-13 (PeproTech), and IL-10 (PeproTech); chemokines, including CCL11 (PeproTech), CXCL1 (R&D Systems, Minneapolis, MN, USA), CCL5 (R&D Systems), and CCL17 (R&D Systems); and growth factors, including VEGF (PeproTech) and TGF-β (BioLegend, San Diego, CA, USA), in BALF from all animal groups were quantified by enzyme-linked immunosorbent assay (ELISA), as per the manufacturer’s protocol.
da Silva A.L., de Oliveira G.P., Kim N., Cruz F.F., Kitoko J.Z., Blanco N.G., Martini S.V., Hanes J., Rocco P.R., Suk J.S, & Morales M.M. (2020). Nanoparticle-based thymulin gene therapy therapeutically reverses key pathology of experimental allergic asthma. Science Advances, 6(24), eaay7973.
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7

Cytokine Quantification Protocols

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ELISAs were performed to measure the supernatant concentrations of human CCL4 (R&D Systems, Minneapolis, Minn), CXCL10 (BD PharMingen), and CCL11 (PeproTech, Rocky Hills, NJ). The CCL5 ELISA used a rabbit anti-human CCL5 antibody (Endogen, Woburn, Mass) with a goat anti-human secondary antibody (R&D Systems). LTC 4 levels were measured according to a modification of the protocol from Volland et al. 46 A commercial high-sensitivity amplification system (Invitrogen) was used for all ELISAs. For detection of IFN-α2 and IFN-β in supernatants and selected CXCL10 measurements, Luminex assays were used (eBioscience and Bio-Rad Laboratories, Hercules, Calif).
Al-Afif A., Alyazidi R., Oldford S.A., Huang Y.Y., King C.A., Marr N., Haidl I.D., Anderson R, & Marshall J.S. (2015). Respiratory syncytial virus infection of primary human mast cells induces the selective production of type I interferons, CXCL10, and CCL4. The Journal of allergy and clinical immunology, 136(5).
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