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Aicar a9978

Manufactured by Merck Group
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AICAR (A9978) is a laboratory research chemical produced by Merck Group. It is a synthetic compound with the chemical formula 5-Aminoimidazole-4-carboxamide ribonucleotide. AICAR is primarily used as a research tool in scientific studies, particularly in the field of cellular metabolism and signaling pathways.

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Lab products found in correlation

Cycloheximide c4859, by Merck Group (1 mentions) Phenformin p7045, by Merck Group (1 mentions) Anti lamin a c sc 6215, by Santa Cruz Biotechnology (1 mentions) Rapamycin r 5000, by LC Laboratories (1 mentions) Anti ha antibody mms 101r, by Fortrea (1 mentions) Mg132 m 1157, by AG Scientific (1 mentions) Necrosulfonamide, by Merck Group (1 mentions) Z vad fmk, by Bio-Techne (1 mentions) Oligomycin a 75351, by Merck Group (1 mentions) Anti c myc, by Cell Signaling Technology (1 mentions) Anti p ampkα thr172, by Cell Signaling Technology (1 mentions) Anti ubc9, by Cell Signaling Technology (1 mentions) Anti mcp 1, by Cell Signaling Technology (1 mentions) Anti ampkα, by Cell Signaling Technology (1 mentions) Anti ampkα1, by Cell Signaling Technology (1 mentions) Anti α smooth muscle actin sma, by Cell Signaling Technology (1 mentions) Anti sumo2 3, by Cell Signaling Technology (1 mentions) Anti ampkα2, by Cell Signaling Technology (1 mentions) Anti il 6, by Cell Signaling Technology (1 mentions) Non targeting sirna 1, by Horizon Discovery (1 mentions)

Spelling variants of this product

AICAR (157 citations) A9978 (3 citations)

4 protocols using aicar a9978

1

Analysis of AMPK and autophagy regulation

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The following commercially available antibodies were used: anti-AMPKα1 (ab110036), anti-AMPKα2 (ab3760), anti-ATG14 (ab173943), anti-FOXO3a (ab12162), anti-histone H3 (ab1791), anti-H3R17me2 (ab8284), anti-H3K4me3 (ab8580), anti-H3K9me3 (ab8898), anti-H3K36me3 (ab9050), anti-PI3K class 3 (ab124905), and anti-TFEB (ab2636) antibodies were purchased from Abcam. Anti-AMPK (2532), anti-ATG12 (4180), anti-CARM1 (3379 for immunblotting, 12495 for immunoprecipitation and ChIP), anti-LC3 (2775), anti-phospho-AMPKαT172 (2535), anti-phospho-FOXO3a S413 (8174), anti-SQSTM1/p62 (5114), and anti-TFE3 (14779) antibodies were from Cell Signaling Technology. Anti-SKP2 (sc-7164), anti-CUL1 (sc-17775), anti-tubulin (sc-8035), and anti-Lamin A/C (sc-6215) were from Santa Cruz Biotechnology. Anti-Flag (F3165), anti-ULK1 (A7481) and anti-β-actin (A1978) antibodies were from Sigma, anti-HA antibody (MMS-101R) from Covance, and anti-tubulin antibody (LF-PA0146A) from Abfrontier. The following chemicals were used in this study: rapamycin (R-5000) was purchased from LC laboratories, cycloheximide (C4859), AICAR (A9978) and phenformin (P7045) from Sigma, bafilomycin A1 (11038) and ellagic acid from Cayman (10569), compound C from Calbiochem (171260), and MG132 (M-1157) from A.G. Scientific.
Shin H.J., Kim H., Oh S., Lee J.G., Kee M., Ko H.J., Kweon M.N., Won K.J, & Baek S.H. (2016). AMPK–SKP2–CARM1 signalling cascade in transcriptional regulation of autophagy. Nature, 534(7608), 553-557.
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2

Cellular Metabolic Regulation Assay

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Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) (C2759), Oligomycin A (75351) and AICAR (A9978) were from Sigma–Aldrich (St Louis, MO, USA). Recombinant Human TNF-α (210-TA-020, R&D systems), 5-Aminoindole for Smac mimetic (A59654, Sigma–Aldrich), Z-VAD-FMK (2163, Tocris Bioscience) and Necrosulfonamide (480073, EMD Millipore Corporation, Billerica, MA, USA) were used. The following antibodies were used: polyclonal rabbit anti-phospho MLKL antibody (Thr-357) (ABC234, EMD Millipore Corporation, Billerica, MA, USA), polyclonal rabbit anti-MLKL antibody (M6697, Sigma–Aldrich), AMPK and ACC antibody sampler kit for phospho-AMPKα and AMPKα (#9957, Cell Signaling Technology), Total OXPHOS Rodent WB antibody cocktail for mitochondrial electron transport chains (ab110413, Abcam), polyclonal rabbit anti-Tom20 Antibody (sc-11415, Santa Cruz Biotechnology), and monoclonal mouse anti-β-actin (A5316, Sigma–Aldrich).
Koo M.J., Rooney K.T., Choi M.E., Ryter S.W., Choi A.M, & Moon J.S. (2015). Impaired oxidative phosphorylation regulates necroptosis in human lung epithelial cells. Biochemical and biophysical research communications, 464(3), 875-880.
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3

AMPK and GPR120 Signaling Pathway

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Anti-tGFP (TA150039) was purchased from OriGene (Rockville, MD). Anti-AMPKα (#5831), anti-pAMPKα-Thr172 (#2535), Anti-AMPKα1 (#2795), Anti-AMPKα2 (#2757), anti-c-myc (#5605), anti-pc-myc-Ser62 (#13748), anti-UBC9 (#4786), anti-IL-6 (#13797), anti-NaK ATPase (#7074), anti-α-smooth muscle actin (SMA; #56856), anti-SUMO2/3 (#4971), and anti-MCP-1 (#12838) were purchased from Cell Signaling Technology Inc. (Danvers, MA). Anti-β-arrestin 2 (C16D9) rabbit mAb (Cell Signaling Technology) for the western blotting analysis, and sc-365445 (Santa Cruz Biotechnology) for the IP assay. Anti-β-arrestin 1 (D8O3J) rabbit mAb (Cell Signaling Technology). Anti-α-actin (sc-47778) and anti-SUMO1 (sc-5380) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). Anti-GPR120 (sc-390752) and anti-SUMO2/3 immunoprecipitation (IP) beads (#BK-162) were purchased from Cellskeleton, Inc and antibody (sc-50331) were purchased from Santa Cruz Biotechnology, Inc. GW9508 (G9797) and AICAR (A9978) were purchased from Sigma-Aldrich (St. Louis, MO). DHA (CAS6217-54-5) was purchased from Cayman Chemical Company (Ann Arbor, MI). Anti-CD68 (14-0681-80) was purchased from Affymetrix-ebioscience.
Yan C.H., Liu H.W., Tian X.X., Li J., Ding Y., Li Y., Mei Z., Zou M.H, & Han Y.L. (2022). AMPKα2 controls the anti-atherosclerotic effects of fish oils by modulating the SUMOylation of GPR120. Nature Communications, 13, 7721.
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4

Molecular Pathways Regulation in Cancer

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Antibodies to TBP (ab51841), c-Fos (4384), GAPDH (2118), p65 (4764), N-cadherin (4061), E-cadherin (3195), AKT (4685), p-AKT (4058), Phospho-AMPKα (Thr172) (2535), AMPKα (2603), TWIST (SC-15393), FBXL10 (ab5199, ab64920), c-FLIPL (06-864), cleaved Caspase 3 (9661) were purchased from Abcam, Cell Signaling and Santa Cruz. Metformin (D150959) and Doxorubicin hydrochloride (D1515), Bay11-7085 (B5681), AICAR (A9978) and Monoclonal Anti-N-Cadherin, clone GC-4 (C3865) were purchased from Sigma. shRNA AMPKα1 (TRCN0000000858), shRNA N-cadherin (TRCN0000053978), siRNA AMPK α1 (M-005027-02-0005), siRNA N-cadherin (M-011605-01-0005), Non-Targeting siRNA #1 (D-001210-01-05) and siRNA TWIST1 (M-006434-02-0005) were purchased from Dharmacon. siRNA II NF-κB p65 (6534) was from Cell Signaling. Nuclear extraction was prepared according to the manufacturer's instruction (Pierce Biotechnology).
Ge R., Wang Z., Wu S., Zhuo Y., Otsetov A.G., Cai C., Zhong W., Wu C.L, & Olumi A.F. (2015). Metformin represses cancer cells via alternate pathways in N-cadherin expressing vs. N-cadherin deficient cells. Oncotarget, 6(30), 28973-28987.
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