Anhydrous dimethylformamide

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anhydrous dimethylformamide is a clear, colorless liquid commonly used as a solvent in various laboratory applications. It is primarily utilized for its high solvency power and ability to dissolve a wide range of organic and inorganic compounds.

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Lab products found in correlation

8 protocols using anhydrous dimethylformamide

Perovskite Solar Cell Fabrication

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The following materials were used: formamidinium
iodide (Sigma, >99%), PbI₂ (Sigma, 99%), phenylethylammonium
iodide (Greatcell Solar Materials), anhydrous dimethylformamide (99.8%,
Acros), dimethylsulfoxide (99.7%, Acros), acetone (99.6%, Acros),
chlorobenzene (99.8%, Acros), isopropanol (99.5%, Acros), and d₅-FAI (Cortecnet, 85% CD deuterated, 90% ND₂ deuterated). d₄-FAI and d₃-PEAI were prepared by dissolving in heavy
water (1:40 mol/mol ratio), followed by evaporation. This yielded
∼50 and ∼70% deuterium on the FAI and PEAI, respectively.

Mishra A., Hope M.A., Almalki M., Pfeifer L., Zakeeruddin S.M., Grätzel M, & Emsley L. (2022). Dynamic Nuclear Polarization Enables NMR of Surface Passivating Agents on Hybrid Perovskite Thin Films. Journal of the American Chemical Society, 144(33), 15175-15184.

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Sonogashira Coupling Characterization

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The ¹H and ¹³C NMR spectra were recorded on Bruker instruments at 400 MHz. Chemical shifts are reported in ppm and are referenced to residual DMSO solvent; 2.50 and 39.51 ppm for ¹H and ¹³C respectively. The high-resolution mass spectrometry was provided by University of Connecticut Mass Spectrometry Laboratory using AccuTOF mass spectrometer with a DART source. Optical rotation was measured on a Jasco P-2000 polarimeter at 589nm. TLC analyses were performed on Sorbent Technologies silica gel HL TLC plates. All glassware was oven-dried and allowed to cool under an argon atmosphere. Anhydrous dichloromethane, ether, and tetrahydrofuran were used directly from Baker Cycle-Tainers. Anhydrous dimethylformamide was purchased from Acros and degassed by purging with argon. All reagents were used directly from commercial sources unless otherwise stated. A premixed heterogeneous mixture of CuI (10%/w) in Pd(PPh₃)₂Cl₂–(Pd/Cu) was used for the Sonogashira coupling.

Reeve S.M., Scocchera E.W., G-Dayanadan N., Keshipeddy S., Krucinska J., Hajian B., Ferreira J., Nailor M., Aeschlimann J., Wright D.L, & Anderson A.C. (2016). MRSA isolates from the United States hospitals carry dfrG and dfrK resistance genes and succumb to propargyl-linked antifolates. Cell chemical biology, 23(12), 1458-1467.

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HPLC Purity Determination and Radiolabeling

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The purity of all final compounds was determined by HPLC analysis and showed purity >95%. Compound 1 (CF53) was purchased form MedChemExpress; anhydrous dimethylformamide (DMF) was purchased from Acros Organics. The analytical separation was conducted on an Agilent 1100 series HPLC fitted with a diode-array detector, quaternary pump, vacuum degasser, and autosampler. Mass spectrometry data were recorded on an Agilent 6310 ion trap mass spectrometer (ESI source) connected to an Agilent 1200 series HPLC with a quaternary pump, vacuum degasser, diode-array detector, and autosampler.
[¹¹C]CO₂ was produced via the ¹⁴N (p, α) ¹¹C reaction on nitrogen with 2.5% oxygen, with 11 MeV protons (Siemens Eclipse cyclotron), and trapped on molecular sieves in a TRACERlab FX-MeI synthesizer (General Electric). [¹¹C]CH₄ was obtained by the reduction of [¹¹C]CO₂ in the presence of Ni/hydrogen at 350°C and recirculated through an oven containing I₂ to produce ¹¹CH₃I via a radical reaction.
All animal studies were carried out at Massachusetts General Hospital (PHS Assurance of Compliance No. A3596-01). The Subcommittee on Research Animal Care (SRAC) serves as the Institutional Animal Care and Use Committee (IACUC) for the Massachusetts General Hospital (MGH). SRAC reviewed and approved all procedures detailed in this paper.

Bai P., Lan Y., Wang H., Chen Z., Fiedler S., Striar R., Lu X, & Wang C. (2020). Development of a Novel Positron Emission Tomography (PET) Radiotracer Targeting Bromodomain and Extra-Terminal Domain (BET) Family Proteins. Frontiers in Molecular Biosciences, 7, 198.

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Dopamine-PEG Conjugation for Tissue Engineering

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Dopamine
HCl and N-methylmorpholine
were purchased from Acros Organics (Fair Lawn, New Jersey). The 4-arm
10k Da N-hydroxysuccinimide ester activated poly(ethylene
glycol) (PEG-NHS) was purchased from JenKem U.S.A., Inc. (Allen, TX).
Anhydrous dimethylformamide, sodium phosphate monobasic monohydrate,
sodium phosphate dibasic anhydrous, concentrated hydrochloric acid
(36.5–38%), and sodium periodate (NaIO₄) were purchased
from Acros Organics (Fair Lawn, New Jersey). Fresh bovine pericardium
was purchased from Sierra for Medical Science (Whittier, California).
Spectra/Por dialysis membrane (molecular weight cut off (MWCO): 3500
Da) was purchased from Spectrum Laboratories (Rancho Dominguez, California).
Dulbecco’s modified Eagle’s medium (DMEM; with 4.5 g/L
glucose and glutamine, without sodium pyruvate) and trypsin EDTA (0.05%
trypsin/0.53 mM EDTA in HBSS) were obtained from Corning Cellgro (Manassas,
VA). Fetal bovine serum and penicillin-streptomycin (10 units/mL)
were purchase from Thermo Scientific (Rockford, IL). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide 98% (MTT) was purchased from Alfa Aesar (Ward Hill, MA).

Cencer M., Liu Y., Winter A., Murley M., Meng H, & Lee B.P. (2014). Effect of pH on the Rate of Curing and Bioadhesive Properties of Dopamine Functionalized Poly(ethylene glycol) Hydrogels. Biomacromolecules, 15(8), 2861-2869.

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Synthesis of Metal-Organic Complexes

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Phenylenediamine and sodium hydride were purchased from Sigma-Aldrich. Urea was obtained from AnalaR. Ethyl bromoacetate and anhydrous dimethylformamide (DMF) were purchased from ACROS Organics. All metal nitrate salts were purchased from Alfa Aesar. Ethylene glycol, DMF and common laboratory solvents were purchased from Fisher Chemical. All reagents were used without any further purification.

Jones C.L., Marsden E.A., Nevin A.C., Kariuki B.M., Bhadbhade M.M., Martin A.D, & Easun T.L. (2017). Investigating the geometrical preferences of a flexible benzimidazolone-based linker in the synthesis of coordination polymers. Royal Society Open Science, 4(12), 171064.

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Synthesis and Purification of Polyamines

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D₂O, CD₃OD, and
CDCl₃ were purchased from Goss Scientific
(UK). Aqueous ammonia (32%), dichloromethane (DCM), dimethylformamide
(DMF), ethanol (EtOH), ethyl acetate, methanol (MeOH), and triethylamine
(TEA) were purchased from VWR (UK). Anhydrous pyridine, anhydrous
dimethylformamide (DMF), and chloroform were purchased from Fisher
Scientific (UK). Acrylonitrile, 1,12-diaminododecane, di-tert-butyl dicarbonate [(Boc)₂O], ethyl trifluoroacetate,
ninhydrin, norspermine (thermine), Raney-Nickel, spermidine, spermine,
sodium hydroxide (NaOH), succinic anhydride, N,N,N′,N′-tetramethyl-O-(1H-benzotriazol-1-yl)uronium hexafluorophosphate
(HBTu), trifluoroacetic acid (TFA), potassium bromide (KBr), and sodium
sulfate (Na₂SO₄) were purchased from Sigma-Aldrich
(UK).
Column chromatography was performed over silica gel 60–120
mesh (purchased from Sigma-Aldrich, UK) using different ratios of
MeOH, EtOH, ethyl acetate, DCM, and Aqueous ammonia (32%) as eluents.
Thin-layer chromatography (TLC) was performed over silica gel using
aluminum-backed sheets coated with Kieselgel 60 F₂₅₄ purchased
from Merck (UK). ninhydrin TLC spray reagent was used for detecting
amine functional groups [ninhydrin (0.2 g) in 100 mL EtOH].

Alkhzem A.H., Li S., Wonfor T., Woodman T.J., Laabei M, & Blagbrough I.S. (2022). Practical Synthesis of Antimicrobial Long Linear Polyamine Succinamides. ACS Bio & Med Chem Au, 2(6), 607-616.

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PEG-Hyaluronic Acid Hydrogel Synthesis

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The 4 kDa poly(ethylene glycol) (PEG) was purchased from Fluka (Buchs, Switzerland). Sodium hyaluronate (1.5 MDa) was purchased from Lifecore Biomedical (Chaska, MN, USA). Dexamethasone (DX), chloroacetyl chloride, 1,1′-carbonyldiimidazole (CDI), sodium acrylate, triethylamine (TEA), 4-methoxyphenol, anhydrous sodium sulfate, sodium bicarbonate, and sodium chloride were purchased from Sigma-Aldrich (Milwaukee, WI, USA). Dowex 50WX8 200-400 (H) was purchased from Alfa Aeser (Ward Hill, MA, USA). Anhydrous dichloromethane, anhydrous dimethylformamide, HPLC-grade acetonitrile, chloroform, ethyl ether, and dimethyl sulfoxide (DMSO) were purchased from Fisher Chemical (Fair Lawn, NJ, USA). The 2-hydroxy-1-[4-(hydroxyethoxy) phenol]-2-methyl-1-propanone (Irgacure 2959, I-2959) was donated by BASF (Florham Park, NJ, USA).

Macks C., Jeong D., Bae S., Webb K, & Lee J.S. (2022). Dexamethasone-Loaded Hydrogels Improve Motor and Cognitive Functions in a Rat Mild Traumatic Brain Injury Model. International Journal of Molecular Sciences, 23(19), 11153.

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Radiosynthesis of [18F]Tetramethylammonium Fluoride

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HPLC grade acetonitrile, potassium trifluoromethanesulfonate, and anhydrous dimethylformamide were purchased from Fisher Scientific. Sodium bicarbonate, kryptofix^® 2.2.2 (K_2.2.2), and anhydrous acetonitrile were purchased from Sigma-Aldrich. Tetramethylammonium bicarbonate (Me₄NHCO₃) was purchased from Enamine. Tetramethylammonium tetrafluoroborate (Me₄NBF₄) was purchased from TCI America. Sterile product vials (10 mL) were purchased from Hollister-Stier. QMA-light and C18 Lite Sep-Paks were purchased from Waters Corporation. QMA-light Sep-Paks were flushed with isopropanol (10 mL), 0.5 M aqueous Sodium bicarbonate (10 mL), and MilliQ water (10 mL) prior to use for the generation of K¹⁸F and in situ Me₄N¹⁸F. C18 Sep-Paks were flushed with ethanol (10 mL) and sterile water for injection (10 mL) prior to use. High performance liquid chromatography (HPLC) was performed using a Shimadzu LC-2010A HT system equipped with a Bioscan B-FC-1000 radiation detector. Radio-TLC analyses were performed using a Bioscan AR 2000 Radio-TLC scanner with EMD Millipore TLC silica gel 60 plates (3.0 cm wide x 6.5 cm long). Residual TMA levels were determined using TLC spot tests as previously described.^{32 (link)} Chromatography was performed using the following conditions:

Lee S.J., Morales-Colón M.T., Brooks A.F., Wright J.S., Makaravage K.J., Scott P.J, & Sanford M.S. (2021). SNAr Radiofluorination with In Situ-Generated [18F]Tetramethylammonium Fluoride. The Journal of organic chemistry, 86(20), 14121-14130.

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