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Premix wst 1 cell proliferation assay system

Manufactured by Roche
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The Premix WST-1 Cell Proliferation Assay System is a colorimetric assay for the quantitative measurement of cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. The amount of formazan produced is directly proportional to the number of metabolically active cells in the culture.

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WST-1 cell proliferation assay WST-1 proliferation assay WST-1 assay WST assays WST-1

3 protocols using premix wst 1 cell proliferation assay system

1

Cell Proliferation Assay of MDA-MB-231 Cells

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The cell proliferation assay was performed using the Premix WST-1 Cell Proliferation Assay System (Roche Holding AG, Basel, Switzerland) in accordance with the manufacturer's protocol. Briefly, MDA-MB-231 cells containing shNT or shGPR81 RNA were plated in 24-well plates (40,000 cells/well) and incubated at 37 °C in a 5% CO2 atmosphere. On days 1 and 2, cell proliferation reagent was added to each well and incubated for 1 h. The absorbance was measured using the Model 550 micro-plate reader described above.
Ishihara S., Hata K., Hirose K., Okui T., Toyosawa S., Uzawa N., Nishimura R, & Yoneda T. (2022). The lactate sensor GPR81 regulates glycolysis and tumor growth of breast cancer. Scientific Reports, 12, 6261.
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2

Cell Proliferation Assay with WST-1 Reagent

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Cell proliferation assay was done using a premix WST-1 cell proliferation assay system (Roche Applied Science) following the manufacturer’s instructions. Cells were seeded on 96-well plates at a density of 2 × 103 per well in 100 μl culture medium. Cells were incubated for 1 to 4 days and subsequently exposed to 10 μl WST-1 reagent for 2 hours. The absorbance was measured at 450 nm as the detection wavelength and 670 nm as the reference wavelength for the assay.
Nagathihalli N.S., Beesetty Y., Lee W., Washington M.K., Chen X., Lockhart A.C, & Merchant N.B. (2014). Novel Mechanistic Insights into Ectodomain Shedding of EGFR Ligands Amphiregulin and TGF-α: Impact on Gastrointestinal Cancers Driven by Secondary Bile Acids. Cancer research, 74(7), 2062-2072.
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3

Cell Viability Assay via WST-1

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Cell viability was tested with WST-1 reagent, Premix WST-1 Cell Proliferation Assay System (Roche, Basel, Switzerland), according to the manufacturer’s instructions. Briefly, 4 × 10‍3 cells/well were seeded in a 96-well plate and treated with different drugs at various concentrations for the indicated times. After the addition of 10 μl Premix WST-1 solution to each well, cells were incubated at 37°C for another 1 ‍h, and the absorbance was determined at 440 nm using a microplate reader.
Koike T., Takenaka M., Suzuki N., Ueda Y., Mori M., Hirayama T., Nagasawa H, & Morishige K.I. (2022). Intracellular ferritin heavy chain plays the key role in artesunate-induced ferroptosis in ovarian serous carcinoma cells. Journal of Clinical Biochemistry and Nutrition, 71(1), 34-40.
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