Representative TEM images of samples were acquired using scanning transmission electron microscopy-bright field (STEM-BF) imaging with an accelerated voltage of 200 kV using a Tecnai G220S-TWIN electron microscope (FEI, Czech). The infrared spectra of all samples were collected according to a previously published method.40 (link) The magnetization properties and magnetophoretic mobility of all MNPs were measured according to a previously published method.40 (link)
Tecnai g2 20 s twin electron microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Tecnai G2 20 S-TWIN electron microscope is a high-performance transmission electron microscope (TEM) developed by Thermo Fisher Scientific. It is designed to provide advanced imaging and analytical capabilities for a wide range of applications in materials science, nanotechnology, and life sciences research. The Tecnai G2 20 S-TWIN features a dual-lens system, allowing for both low- and high-magnification imaging, and is equipped with a range of state-of-the-art detectors and analytical tools to enable comprehensive materials characterization.
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Lab products found in correlation
D8 advance, by Brucker (1 mentions)
Leo 1530 gemini, by Zeiss (1 mentions)
Sta449f3, by Netzsch (1 mentions)
Zetasizer nano series nano zs, by Malvern Panalytical (1 mentions)
Spectrum one, by PerkinElmer (1 mentions)
Ppms 9, by Quantum Design (1 mentions)
Filter paper, by Cytiva (1 mentions)
Pelco easiglow instrument, by Ted Pella (1 mentions)
4 protocols using tecnai g2 20 s twin electron microscope
1
Characterization of Magnetic Nanoparticles
2
Crystalline Phases and Mullite Synthesis
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Crystalline phases in the final products were identified by powder X-ray diffraction (XRD, D8 Advance, Brucker, Germany) equipped with a Lynx Eye 1D detector and using Co Kα radiation. The diffraction patterns were collected in a Bragg–Brentano geometry. Rietveld refinement was performed using the FULLPROF program.22 The morphology and elemental compositions of the samples were examined by scanning electron microscopy (SEM) in LEO Gemini 1530, Carl (Zeiss, Germany) coupled with an energy dispersive X-ray detector (Thermo Fisher Scientific, USA) on samples sputtered with a gold layer. Transmission electron microscopy (TEM) images of mullite whiskers were obtained on an FEI Tecnai G2 20 S-TWIN electron microscope equipped with an energy dispersive X-ray detector (EDX) operated at 200 kV (FEI, USA).
To address the formation mechanisms of mullite, thermogravimetric (TG) and differential thermal analysis (DTA) were performed under a mixture atmosphere of oxygen and argon atmosphere (20% O2 – 80% Ar) using STA 449F3 (Netzsch, Germany).
To address the formation mechanisms of mullite, thermogravimetric (TG) and differential thermal analysis (DTA) were performed under a mixture atmosphere of oxygen and argon atmosphere (20% O2 – 80% Ar) using STA 449F3 (Netzsch, Germany).
3
Characterization of Superparamagnetic Iron Oxide Nanoparticles
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The size and morphology were visualised using a FEI Tecnai G2-20S-TWIN electron microscope (Hillsboro, USA). The hydrodynamic size and zeta potential of materials were measured with a Zeta Sizer Nano series Nano-ZS (Malvern Instruments Ltd, Malvern, UK). The super paramagnetic properties of iron oxide nanoparticles were analyzed using PPMS-9 (Quantum Design Inc, San Diego, USA). The Fe elemental content was measured using ICP-OES (PE8000, PerkinElmer, Waltham, USA). FTIR spectra of nanoparticles were collected with a Fourier transform infrared spectrometer (Spectrum One, PerkinElmer, Waltham, USA).
4
Transmission Electron Microscopy of PLD Aggregation
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Samples containing 50 µM PLD and MetO PLD were incubated in turbidity buffer at 37 °C in an orbital shaker operated at 300 rpm. For TEM specimen preparation, 5 µl of 3, 5, 10, and 20 days aged PLD and MetO PLD samples were placed onto carbon-coated, formvar 300 mesh copper grids (Ted Pella) that were freshly glow discharged with a PELCO easiGlow instrument (Ted Pella). After 1 min incubation at room temperature, the solvent excess was blotted with filter paper (Whatman). The grids were next stained with 5 µl of 2% (w/v) uranyl acetate in water for 1 min. After blotting to remove the excess of stain, the grids were dried before imaging. Micrographs were acquired using a Gatan ORIUS SC600 (Model 831) digital camera on a FEI Tecnai G2 20 S-TWIN electron microscope that was operated at 200 kV. Images were replicated four times.
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