Life technologies

Golden gate cloning followed the principles outlined in Engler et al.^{28 (link),29 (link)}. Level 0 modules were synthesised by Life Technologies™ (ThermoFisher Scientific) and assembled as described in Supplementary Table 2.

A total of 30 mg of shredded material present in tea bags from each package was used for DNA extraction. Aiming at selecting the best DNA isolation procedure to obtain high yield and quality of extracted DNA [42 ], a preliminary analysis on three samples (green, black and admixture tea) was performed using four commercial kits {PowerPlant Pro DNA Isolation Kit (Mo Bio), Plant Genomic DNA Extract Mini Kit (Fisher Molecular Biology), ZR Plant/Seed DNA MicroPrep (Zymo Research), GeneAll Exgene Plant SV kit (GeneAll Biotechnology)} and two detergent protocols [43 , 44 (link)]. The isolated DNA was analyzed using both a spectrophotometer Nanodrop 2000 (Thermo Fisher Scientific) to quantify its purity grade (260/280 and 260/230) and a Qubit 3 Fluorometer to determine the precise DNA concentration (Life Technologies, Thermo Fisher Scientific). In addition, a visual estimate was obtained using 1% agarose electrophoresis with Gel Red strained (Biotium) band intensities and GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific). After preliminary analysis, all extracted genomic DNA were estimated using both fluorometric and electrophoresis analyses.
For fresh plant references from BGN, 100 mg of leaf tissue was used for the DNA extraction using GeneAll Exgene Plant SV kit (GeneAll Biotechnology) according to the manufacturer's instructions.

Cells were seeded in 24‐well transparent plates (Life Technologies; Thermo Fisher Scientific, Inc.) at a density of 5 × 10³ cells per well and were grown to achieve 80% confluency overnight. Then, cells were treated with alliin (25, 50, 100 μM) for 24 h, immunofluorescence staining was performed as previously described.²⁰

IgH and IgL DNA fragments coding for human MDE8 (41 (link)) antibody were prepared by PCR-amplification from codon-optimized synthetic genes (Life Technologies, Thermo Fisher Scientific). Purified digested DNA fragments were cloned into human Igγ1-and Igλ-expressing vectors (42 (link)), and human MDE8 IgG1 antibod-ies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described (43 (link)). Recombinant IgG1 antibodies were purified by batch/gravity-flow affinity chromatog-raphy using protein G sepharose 4 fast flow beads (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions, extensively dialyzed against PBS using Slide-A-Lyzer^® dial-ysis cassettes (Thermo Fisher Scientific) and quantified using NanoDrop 2000 instrument (Thermo Fisher Scientific) (43 (link)).

The liver and placental labyrinthine zone (50–100 mg) were reduced in 1 ml of TRizol^TM Reagent (15596; Thermo Fisher Scientific, CA, United States) with mixer Mill MM300 (Qiagen, Germantown, MD, United States) and one tungsten ball for 2 min at 20 Hz twice. Then, total RNA was extracted according to the instructions of the manufacturer (Life technologies, Thermo Fisher Scientific, CA, United States). After RNA precipitation with 500 μl of isopropanol and washing with 1 ml of 70% ethanol, total RNA was resuspended in RNAse free water and stored at −20°C. RNA concentration and purity (A260/A280 and A260/A230) were measured using a NanoDrop spectrophotometer (Nanodrop technology, Thermo Fisher Scientific, CA, United States). RNA degradation was checked by 1.5% agarose gel migration. RNA purification and DNase treatment were performed with ReliaPrep RNA tissue Miniprep Systems Kit (Z6110; Proméga, La Farlède, France). Another measure using the Nanodrop spectrophotometer was performed. Then, the quality of purified RNA was assessed using Bio-Analyzer Agilent 2100 (Agilent Biotechnologies). The RNA integrity number (RIN) of the samples was within the range of 7.3 and 10.