Mucin from the porcine stomach

Manufactured by Merck Group

Sourced in United States, China

Mucin from the porcine stomach is a naturally occurring glycoprotein extracted from the stomach lining of pigs. It serves as a key component in various research and laboratory applications. The product provides a source of purified mucin that can be utilized for diverse experimental purposes.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Mucin (95 citations) Porcine mucin (53 citations) Mucin from porcine stomach (40 citations) Porcine stomach mucin (16 citations) Porcine stomach (9 citations) Mucin from (6 citations) Type II mucin from the porcine stomach (2 citations)

4 protocols using mucin from the porcine stomach

Chitosan-based Formulation for Improved Oral Bioavailability

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chitosan was obtained from Tokyo Chemical Industry, while WPI and MCTs were provided by Shanghai Yuanye Bio-Technology Co., Ltd. EO was purchased from J&K Scientific Ltd. CO was purchased from Innochem Co., Ltd. Apigenin, glycerol, and mucin from the porcine stomach, pepsin from porcine gastric mucosa (250 units/mg solid), bile salts and lipase from porcine pancreas (100–650 units/mg protein) were purchased from Sigma-Aldrich (Sigma Chemical Co. St. USA). All the solvents and reagents were of analytical grade. The double-distilled water used in this study was obtained from a water purification system (Direct-Pure-EDI 15UV).

Ge R., Zhu H., Zhong J., Wang H, & Tao N. (2022). Storage stability and in vitro digestion of apigenin encapsulated in Pickering emulsions stabilized by whey protein isolate–chitosan complexes. Frontiers in Nutrition, 9, 997706.

+ Open protocol

+ Expand

Artificial Saliva Preparation for Viral Surrogates

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used B. atrophaeus spores (ATCC 9372; Crosstex, USA) as a surrogate or validation for coronavirus. Sterile techniques were used throughout. Spore strip, suspension handling and recovery procedures were carried out in a BSL-2 laboratory (Center for Applied NanoBioscience and Medicine, The University of Arizona). Human coronavirus, HCoV-NL63, and SARS-CoV-2 handling and recovery procedures were carried out in a BSL-3 laboratory (ZeptoMetrix Corporation Buffalo, NY; in collaboration with iFyber, LLC, Ithaca, NY). The artificial saliva was prepared using the ingredients listed in Table 1(Woo et al., 2010 (link)). Saliva is an extracellular fluid in the mouth consisting of 98⁠% water plus electrolytes, mucosal proteins, other proteins, and enzymes (Diaz-Arnold and Marek, 2002 (link)) which maintain osmotic balance (Dodds et al., 2005 (link)). Mucin from the porcine stomach (Sigma–Aldrich, St. Louis, MO) was used for mimicking mucus stimulant. All other artificial saliva components were analytical grade from Sigma–Aldrich.Table 1

Artificial saliva composition.

Table 1

S.No	Chemical species	Amount for 97.9 mL DI water
1	MgCl₂ . 7H₂O	4 mg
2	CaCl₂ . H₂O	13 mg
3	(NH₂)₂CO	12 mg
4	NaHCO₃	42 mg
5	NaCl	80.8 mg
6	KCl	104 mg
7	Dulbecco's modified Eagle's medium (DMEM)	0.1 mL
8	Mucin	300 mg
9	NH₄Cl	11 mg
10	0.2 M KH₂PO₄	0.770 mL

Bennet D., Harris A.F., Lacombe J., Brooks C., Bionda N., Strickland A.D., Eisenhut T, & Zenhausern F. (2021). Evaluation of supercritical CO2 sterilization efficacy for sanitizing personal protective equipment from the coronavirus SARS-CoV-2. The Science of the Total Environment, 780, 146519.

+ Open protocol

+ Expand

Analytical Characterization of Tea Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

(-)-Epicatechin gallate (ECG, chromatographically pure) and (-)-epigallocatechin gallate (EGCG, chromatographically pure) were purchased from Taiyo Greenpower Co., Ltd. (Wuxi, China). Mucin (from the porcine stomach), (-)-epigallocatechin (EGC), (-)-epicatechin (EC), caffeic acid, chlorogenic acid, gallic acid and rutin were purchased from Sigma-Aldrich (Shanghai, China). Potassium chloride (KCl), calcium chloride (CaCl₂), magnesium chloride (MgCl₂) and aluminum chloride (AlCl₃) were all analytically pure and were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). We used a Hach TL2300 Turbidimeter (Shanghai, China). Tea samples were obtained from the Tea Research Institute, Chinese Academy of Agricultural Sciences (Hangzhou, China). The brewing water used was pure water obtained from Hangzhou Wahaha Group Co., Ltd. (Hangzhou, China).

Xu L., Ye Q., Cao Q., Liu Y., Li X., Liu Z., Gong Y., Zhang S., Yin J, & Xu Y. (2024). Effects of the Taste Substances and Metal Cations in Green Tea Infusion on the Turbidity of EGCG–Mucin Mixtures. Foods, 13(8), 1172.

+ Open protocol

+ Expand

Antimicrobial Activity Evaluation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gold(III) chloride trihydrate and Lam (CAS number 9008–22-4), mucin from the porcine stomach (CAS number 84082–64-4), diethylene triamine penta-acetic acid (DTPA), salmon sperm DNA, and Congo red were received from Sigma-Aldrich Co. (St. Louis, MO, USA). The casamino acid and egg yolk emulsion (Cat. No. MB-E1864) were purchased from MBcell (Kisan Bio Co., Ltd., South Korea). S. aureus (KCTC1916), P. aeruginosa PAO1 (KCTC1637), Listeria monocytogenes (KCTC3569), and Escherichia coli (KCTC1682) were bought from the Korean Collection for Type Cultures (KCTC, Daejeon, South Korea). The Korean Culture Center of Microorganisms (KCCM; Seodaemun-gu, Seoul) provided the Streptococcus mutans (KCCM40105) and Candida albicans (KCCM11282), while the American Type Culture Collection supplied the K. pneumoniae (ATCC4352). All the bacterial cells were cultured in tryptic soy broth (TSB), whereas C. albicans was cultivated in potato dextrose broth (PDB). The growth temperature for these microbes was 37 °C.

Tabassum N., Khan F., Jeong G.J., Oh D, & Kim Y.M. (2024). Antibiofilm and antivirulence activities of laminarin-gold nanoparticles in standard and host-mimicking media. Applied Microbiology and Biotechnology, 108(1), 203.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!