B6.129s1 stat3tm1xyfu j stat3fl fl

Animal work was carried out in accordance to regulations of the UK Home Office under the Animals (Scientific Procedures) Act 1986, with Local Ethical Review by the Imperial College London Animal Welfare and Ethical Review Body Standing Committee (AWERB). B6(Cg)-Ncf1^m1J/J (Ncf1^−/−, Jackson), B6.129S-Cybb^tm1Din/J (gp91phox^−/−/NOX2^−/−, Jackson), B6(Cg)-Cybbtm1.1Abk/J (NOX2^fl/fl,^{34 (link)}), B6.129S1-Stat3tm1Xyfu/J (STAT3^fl/fl, Jackson), LysM-Cre-NOX2^−/−, and corresponding WT littermates or C57Bl6/J (Harlan, UK) mice ranging from 4 to 8 weeks of age were used for all experiments. For generation of macrophage-specific NOX2^−/− mice, NOX2^fl/fl mice were crossed with LysM-Cre mice as previously described^{15 (link)}. For all surgeries, mice were anesthetized with isoflurane (5% induction 2% maintenance) and a mixture of buprenorphine (0.1 mg/kg) and carprophen (5 mg/kg) was administered peri-operatively as analgesic.

SV129/Bl6.ICER/CREM^−/−mice were originally cloned by Guenther Schuetz, DKFZ Heidelberg36 (link). Animals were crossed to C57BL/6J mice for over nine generations to transfer the ICER/CREM^−/− locus to the B6 background. Female B6.MRL-Faslpr/J (B6.lpr), C57BL/6-Il17atm1Bcgen/J (IL-17GFP), STOCK Tg(Cd4-cre)1Cwi/BfluJ (CD4^cre+), MRL/MpJ-Fas^lpr/J and B6.129S1-Stat3tm1Xyfu/J (STAT3^fl/fl) mice were purchased from The Jackson Laboratory. B6.lpr.ICER/CREM^−/− mice were made by crossing B6.ICER/CREM^−/− mice with B6.lpr mice. CD4^cre+. STAT3^fl/fl mice were made by crossing STAT3^fl/fl mice with CD4^cre+ mice. B6.ICER/CREM^−/−.IL-17GFP mice were made by crossing B6.ICER/CREM^−/− mice with IL-17GFP mice. Animals were killed at the end of their 8–12 weeks of life for in vitro culture experiments and indicated week for in vivo experiments. All mice were maintained in an SPF animal facility (Beth Israel Deaconess Medical Center). Experiments were approved by the Institutional Animal Care and Use Committee of BIDMC. All mice were genotyped to validate claimed strain.