The assay was performed using Wako Glucose Kit (Wako Diagnostics, Mountain View, CA, USA) according to the manufacturer’s protocol. In brief, 250 μL of reconstituted Wako glucose reagent was added to a 2 μL sample in a 96-well plate and incubated with shaking at 37 °C for 5 min. The absorbance was measured at 505 nm using a SpectraMax M5 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). The change in absorbance between samples with or without treatment indicated the glucose uptake of cells.
Wako glucose kit
Manufactured by Fujifilm
Sourced in United States, Japan
The Wako Glucose Kit is a laboratory reagent used for the quantitative determination of glucose in various sample types. It employs an enzymatic method to measure glucose levels.
Automatically generated - may contain errors
3 protocols using wako glucose kit
1
Glucose Uptake Assay Protocol
2
Sake Amino Acid Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thirteen premium Japanese sake samples brewed from Yamadanishiki rice cultivar were selected from Annual National New Sake Awards entries (National Research Institute of Brewing (NRIB) competition, 2011), as described previously [6 (link)]. For glucose quantification, a Wako glucose kit (Wako Pure Chemical Industries Ltd., Osaka, Japan) was used. For pyruvic acid quantification, F-kit pyruvate 8000J was purchased from J.K. International Inc. (Tokyo, Japan). Coomassie brilliant blue (CBB) R-250 was purchased from Nacalai Tesque Inc. (Kyoto, Japan). Other chemicals were purchased from Wako Pure Chemical Industries Ltd.
3
Glucose Quantification Using Wako Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glucose assay were done according to the instructions of assay kit (Wako Glucose kit, Wako Diagnostics, Mountain View, CA). In brief, a 250 μl of reconstituted Wako glucose reagent was added to a 96-well assay plate followed with 2 μl sample addition in each well. The plate was incubated at 37°C for 5 min. The change in absorbance, which indicates the amount of glucose present, was measured at 505 nm and 600 nm by using a spectrophotometer (SpectraMax M5; Molecular Devices, Sunnyvale, CA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!