Serum samples were obtained as described previously. Serum levels of troponin I was measured the by Immulite 2500 (Siemens Medical Solutions, Holliston, Massachusetts), with enzyme-labeled chemiluminescent immunometric assay method (reference interval 0-0.04 ng/mL). Serum BNP concentrations were determined using a chemiluminescent immunoassay method (Immulite 2500; Siemens Medical Solutions, with a reference level of 0-100 pg/mL).
Immulite 2500
Manufactured by Siemens
Sourced in Germany
The Immulite 2500 is an automated immunoassay analyzer designed for in vitro diagnostic testing. It is capable of performing a variety of immunoassays to detect and measure different analytes in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Modular analytics p800, by Roche (2 mentions)
Human igf 1 quantikine elisa kit, by R&D Systems (2 mentions)
Immulite 200 xpi, by Siemens (2 mentions)
Xn 3000, by Sysmex (2 mentions)
Modular p800 analyzer, by Roche (1 mentions)
Variant 2 hemoglobin testing system, by Bio-Rad (1 mentions)
Dxc600i, by Beckman Coulter (1 mentions)
Modular p800, by Roche (1 mentions)
Au2700, by Olympus (1 mentions)
Bn 2 analyzer, by Siemens (1 mentions)
Cobas 6000 analyzer, by Roche (1 mentions)
Modular analytics p800 system, by Roche (1 mentions)
Ids isys, by Immunodiagnostic Systems (1 mentions)
Architect c8000 system, by Abbott (1 mentions)
Variant 2 analyzer, by Bio-Rad (1 mentions)
34 protocols using immulite 2500
1
Serum Biomarkers for Cardiac Assessment
2
Glucose, Insulin, and C-Peptide Assay Methodology
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Glucose concentration was determined by the glucose oxidase method (Yellow Springs Instrument, Yellow Springs, OH). For the first 36 participants, insulin was measured with a solid-phase, two-site, chemoluminescent sandwich immunometric assay (Immulite 2500; Siemens) (the mean [SD] coefficient of variation [CV] was 11.5% for low concentration [6.2] and 8.9% for high concentration [29]), and C-peptide was measured with a solid-phase, two-site competitive sandwich immunometric assay linked to a chemiluminescent marker (Immulite 2500; Siemens) (CV 7.7% for low concentration [1.12] and 8.3% for high concentration [5.94]). For the remaining 65 participants, insulin and C-peptide levels were measured by electrochemiluminescence immune assay (cobas 6000 analyzer; Roche Diagnostics). For insulin, the CV was 6.0% for low concentration (23) and 2.5% for high concentration (82), and for C-peptide the CV was 2.6% for low concentration (1.83) and 2.8% for high concentration (9.51).
3
Assessing Insulin Resistance and Secretion
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Fasting plasma glucose concentrations and glycated hemoglobin levels were determined by enzymatic and colorimetric methods (Roche Modular Analytics P800, Roche Diagnostics, Mannheim, Germany; CV < 5%) and serum insulin concentrations were determined by an immunometric method (Siemens Immulite 2500, Siemens Healthcare Diagnostics, Breda, The Netherlands; CV < 5%). All analyses were performed in the central clinical chemical laboratory of the Leiden University Medical Center. Fasting glucose and insulin levels were used to calculate the Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index as a marker for hepatic insulin resistance. The HOMA-IR was calculated using (fasting insulin * fasting glucose)/22.5. The Insulinogenic Index was calculated as (insulin concentration30min − fasting insulin concentration)/(glucose concentration30min − fasting glucose concentration) and the Matsuda Index as 1000/(squared root (fasting glucose * fasting insulin) * (average glucose * average insulin)) (Singh and Saxena, 2010 (link)).
4
Cardiometabolic Factors and Physical Activity
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Self-reported ethnicity was categorized into white and other, education into low and high (higher vocational school, university and postgraduate education) and tobacco smoking into never (reference), former and current smoker. Physical activity was expressed in metabolic equivalents of task (MET)-hours per week. Data on the frequency, duration and intensity of physical activity during leisure time was collected using the Short Questionnaire to Assess Health-enhancing physical activity (SQUASH). In women, the use of hormones (contraceptives or hormone replacement therapy) was grouped into current and no use of estrogens and menopausal state into postmenopausal (reference), peri-and premenopausal. Data on anthropometry and blood pressure and blood samples were obtained in the morning after an overnight fast of at least 10 h. Hypertension was defined as systolic blood pressure !140 mmHg and/or diastolic blood pressure !90 mmHg, and/or use of antihypertensive drugs [35] . Fasting glucose and insulin were examined using an enzymatic colorimetric assay (Roche Modular P800 Analyzer, Roche Diagnostics, Mannheim, Germany) and a two-site chemiluminescent immunometric assay (Siemens Immulite 2500, Siemens Healthcare Diagnostics, Breda), respectively. More details on the data collection can be found elsewhere [34] .
5
Metabolic Response to Liquid Mixed Meal
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Fasting blood samples were drawn from the antecubal vein after 5 min rest of the participant, after an overnight fast of at least 10 h. Within 5 min after drawing a fasting blood sample, all participants consumed a liquid mixed meal (400 mL) that contained 2.5 megajoule (MJ), of which 16 percent of energy (En%) was derived from protein, 50 En% from carbohydrates and 34 En% from fat. Subsequently, blood samples were drawn after 30 and 150 min. Plasma glucose concentrations were determined by enzymatic and colorimetric methods (Roche Modular Analytics P800, Roche Diagnostics, Mannheim, Germany; CV < 5%) and serum insulin concentrations were determined by an immunometric method (Siemens Immulite 2500, Siemens Healthcare Diagnostics, Breda, The Netherlands; CV < 5%) at the Department of Clinical Chemistry and Laboratory Medicine of the LUMC [21 (link)].
From fasting glucose and insulin concentrations, we calculated the Homeostasis Model Assessment for Insulin Resistance (HOMA-IR), a marker of hepatic insulin resistance [22 (link)]. HOMA-IR was calculated as fasting insulin (μU/mL) × fasting glucose (mmol/L)/22.5 [22 (link),23 (link)]. Matsuda Insulin Sensitivity Index (ISI) was calculated as 10,000/square root (fasting glucose (mg/dL) × fasting insulin (μU/mL)) × (meanglucose0-150 × meaninsulin0-150) [24 (link),25 (link),26 (link)].
From fasting glucose and insulin concentrations, we calculated the Homeostasis Model Assessment for Insulin Resistance (HOMA-IR), a marker of hepatic insulin resistance [22 (link)]. HOMA-IR was calculated as fasting insulin (μU/mL) × fasting glucose (mmol/L)/22.5 [22 (link),23 (link)]. Matsuda Insulin Sensitivity Index (ISI) was calculated as 10,000/square root (fasting glucose (mg/dL) × fasting insulin (μU/mL)) × (meanglucose0-150 × meaninsulin0-150) [24 (link),25 (link),26 (link)].
6
Serum BDNF and IGF-1 Levels in MDD Patients
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In MDD patients and HC, BDNF, and IGF-1 concentrations were evaluated in the morning. Blood was taken by a venous puncture between 8:00 and 11:00 a.m. within the first 2 days after clinical assessments in 73 participants at baseline and 30 patients after 8 weeks of vortioxetine (10‑20 mg per day) treatment. Blood was centrifuged at 3000 g for 10 min, and the serum was stored at -20° C until further processing. IGF-1 was assessed with the chemiluminescence immunoassay Immulite 2500 (Siemens AG, Germany) and Human IGF-1 Quantikine ELISA Kit (R&D Systems Inc., Minneapolis, United States). The measurement range for IGF-1 was from 20 to 1600 ng/ml. BDNF was measured using the chemiluminescence immunoassay «Sunrise» (TEKAN, Austria, GmbH) and BDNF Sandwich ELISA Kit («Millipore-ChemiKineTM»). The measurement range was from 15 to 1000 pg/ml.
7
Pediatric Eczema: Blood Biomarkers
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All thirty children with eczema were offered a complete blood count (CBC), total and food specific IgE (FSIgE) levels for egg white, cow’s milk protein, and wheat. Twenty-two consented, and 40 age and sex matched controls were likewise tested.
Blood samples were collected in Natuashish. CBC’s were run at the Labrador Health Center in Goose Bay. Serum was frozen and transported to the immunology laboratory at the Health Sciences Center, St. John’s for total IgE and FSIgE levels (IMMULITE® 2500 -Siemens).
As per standard we accepted FSIgE levels of ≥0.35 kIU/L to indicate sensitization. This cutoff is far below that required to make a diagnosis of food allergy (6 kIU/L-eggs, 32 kIU/L-milk, and > 100 kIU/L-wheat) [36 (link),37 (link)].
Blood samples were collected in Natuashish. CBC’s were run at the Labrador Health Center in Goose Bay. Serum was frozen and transported to the immunology laboratory at the Health Sciences Center, St. John’s for total IgE and FSIgE levels (IMMULITE® 2500 -Siemens).
As per standard we accepted FSIgE levels of ≥0.35 kIU/L to indicate sensitization. This cutoff is far below that required to make a diagnosis of food allergy (6 kIU/L-eggs, 32 kIU/L-milk, and > 100 kIU/L-wheat) [36 (link),37 (link)].
8
Thyroid Function and TRAb Measurement
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Serum total T3 (reference range: Aalborg 1.1–2.5 nmol/L, Copenhagen 1.0–2.6 nmol/L), and TSH (reference range: Aalborg 0.30–4.5mU/L, Copenhagen 0.40–4.0 mU/L) were measured by automatic routine laboratory immunoassays (Roche Diagnostics Elecsys; Immulite 2500, Siemens). TRAb was measured using a manual, competitive, second-generation RIA (DYNOtest TRAK, Thermo Fisher). TRAb ≥ 1.0 IU/L was considered as TRAb positivity (15 (link)).
9
Troponin I Measurement Protocol
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Venous blood samples were obtained from all patients from an antecubital vein after at least 15 min of semi-supine rest. All blood samples were collected into tubes containing ethylenediaminetetraacetic acid (EDTA) to prevent coagulation. Troponin I concentrations were measured by chemiluminescent immunoassay using an Immulite 2500 (Siemens Medical Solutions, Erlangen, Germany).
10
Blood Sample Collection and Analysis
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Blood samples were obtained using standard techniques, following at least 20 minutes of rest in the supine position. Samples were immediately centrifuged and stored at -80°C before batch analysis. Fasting glucose was measured using a Vitros 5600 Clinical Chemistry analyzer (Ortho Clinical Diagnostics, Raritan, NJ), and fasting insulin was determined using a chemiluminescent immunoassay (Immulite 2500; Siemens Diagnostics).
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