The pre-dosed amalgam capsules were mixed using a high-energy mixer (Silamat S5, Ivoclar Vivadent AG, Liechtenstein) for 5–7 seconds, in accordance with the manufacturers’ instructions, and then transported to the cell culture media according to the groups.19 (link) The weight of a pre-dosed amalgam capsule was 0.4 g.
Silamat s5
Manufactured by Ivoclar Vivadent
Sourced in Liechtenstein, Germany
The Silamat S5 is a mixing device used for mixing dental materials. It features an electronic mixing program and a timer function to ensure consistent mixing results.
Automatically generated - may contain errors
Lab products found in correlation
Dnase 1, by Thermo Fisher Scientific (3 mentions)
Cfx384 machine, by Bio-Rad (1 mentions)
Iscript reverse transcription supermix, by Bio-Rad (1 mentions)
Itaq universal sybr green supermix, by Bio-Rad (1 mentions)
Zirconia silica bead, by Biospec (1 mentions)
Rna aqueous phenol free total rna isolation kit, by Thermo Fisher Scientific (1 mentions)
Iq5 real time pcr detection system, by Bio-Rad (1 mentions)
5 protocols using silamat s5
1
Amalgam Capsule Mixing Protocol
2
Gene Expression Quantification in Arabidopsis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seedlings were frozen in liquid nitrogen and ground with glass beads using a Silamat S5 device (Ivoclar Vivadent) for 10 s. RNA was extracted using TRIzol reagent and treated with DNAse I according to manufacturer instructions (ThermoFisher Scientific). One µg of total RNA was used as template for reverse transcription using iScript Reverse Transcription Supermix as recommended by the manufacturer (BioRad). qPCR was performed with iTaq Universal SYBR®Green Supermix (BioRad) and primers listed in Supplementary Table 1 , in a CFX384 machine (BioRad). Gene expression levels were compared to the reference gene GAPC-2 (At1g13440), ACT2 (At3g18780) or IPP2 (At3g02780).
3
Efficient Protein Extraction from C. glutamicum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C. glutamicum cells were harvested by centrifugation (10,000 g, 4°C, 5 min) during the exponential growth phase and washed twice with 20 mM potassium phosphate buffer (PPB), pH 7.5. One ml of the cell suspension was mixed with 1 g zirconia/silica beads (0.1 mm diameter; Biospec, Bartlesville, USA) in a 2-ml Eppendorf tube and the cells were mechanically disrupted by three 30 s shakings in a Silamat S5 (Ivoclar Vivadent, Ellwangen, Germany). Cell debris and unbroken cells were separated by centrifugation for 5 min at 16,000 g and 4°C. The supernatant was then ultracentrifuged at 171,000 g for 60 min at 4°C. The resulting supernatant containing the soluble proteins and the resuspended sedimented membrane fraction (in 20 mM PPB pH 7.5) were used for the assays.
4
Quantitative Gene Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seeds from five siliques were collected for each cross and frozen together with 1.25- to 1.55-mm glass beads (Carl Roth) in liquid nitrogen. The samples were ground in a Silamat S5 (Ivoclar Vivadent) three times for 7 sec. RNA was extracted using the Ambion RNA aqueous phenol-free total RNA isolation kit according to the manufacturer's instructions. Residual DNA was removed using Invitrogen DNase I (Amplification Grade), and cDNA was synthesized using the Fermentas first strand cDNA synthesis kit according to the manufacturer's instructions. qPCR was performed with an iQ5 real-time PCR detection system (Bio-Rad) and Solis BioDyne-5x Hot FIREPol EvaGreen qPCR Mix Plus (ROX, Solis BioDyne) according to the manufacturer's instructions. qPCR was performed with three biological replicates using the primers indicated in Supplemental Table S3 , and the results were analyzed as described (Simon 2003 (link)) using PP2A as reference gene.
5
Quantification of Plant Anthocyanins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plant seedlings were grown 4 d in the same conditions as for hypocotyl length measurement, and 50 mg plant material was homogenized for 10 s using a Silamat S5 mixer (Ivoclar Vivadent). 250 μl of acidic methanol (1% HCl) was added to each sample and placed in an overhead shaker at 4 °C for 1 h. Samples were centrifuged for 1 min at 14,000 rpm and the supernatant was used to quantify anthocyanin content based on the absorption at 535 and 650 nm. Values were reported as A535 − 0.25 (A650) g−1 fresh weight.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!