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Methocult h4034 optimum

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MethoCult™ H4034 optimum is a methylcellulose-based semisolid culture medium used for the clonal growth and enumeration of human hematopoietic progenitor cells from bone marrow, peripheral blood, or cord blood samples.

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Lab products found in correlation

Mtt reagent, by Thermo Fisher Scientific (2 mentions) 35 mm petri dishes, by BD (2 mentions)

2 protocols using methocult h4034 optimum

1

Colony Forming Assay in Methocult

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1,500 cells were plated in MethoCult™ H4034 optimum medium in 35 mm Petri dishes (Falcon, Cockeysville, MD, USA) in duplicate. Plates were incubated at 37 °C with 5% CO2 and 95% humidity for 7 days. Colonies were scored using an inverted microscope. To visualize the colonies MTT reagent (Thermo Fisher Scientific) was added for 1 h at 37 °C with 5% CO2.
Nachmani D., Bothmer A.H., Grisendi S., Mele A., Bothmer D., Lee J.D., Monteleone E., Cheng K., Zhang Y., Bester A.C., Guzzetti A., Mitchell C.A., Mendez L.M., Pozdnyakova O., Sportoletti P., Martelli M.P., Vulliamy T.J., Safra M., Schwartz S., Luzzatto L., Bluteau O., Soulier J., Darnell R.B., Falini B., Dokal I., Ito K., Clohessy J.G, & Pandolfi P.P. (2019). Germline NPM1 mutations lead to altered rRNA 2’-O-methylation and cause dyskeratosis congenita. Nature genetics, 51(10), 1518-1529.
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2

Colony Forming Assay in Methocult

Check if the same lab product or an alternative is used in the 5 most similar protocols
1,500 cells were plated in MethoCult™ H4034 optimum medium in 35 mm Petri dishes (Falcon, Cockeysville, MD, USA) in duplicate. Plates were incubated at 37 °C with 5% CO2 and 95% humidity for 7 days. Colonies were scored using an inverted microscope. To visualize the colonies MTT reagent (Thermo Fisher Scientific) was added for 1 h at 37 °C with 5% CO2.
Nachmani D., Bothmer A.H., Grisendi S., Mele A., Bothmer D., Lee J.D., Monteleone E., Cheng K., Zhang Y., Bester A.C., Guzzetti A., Mitchell C.A., Mendez L.M., Pozdnyakova O., Sportoletti P., Martelli M.P., Vulliamy T.J., Safra M., Schwartz S., Luzzatto L., Bluteau O., Soulier J., Darnell R.B., Falini B., Dokal I., Ito K., Clohessy J.G, & Pandolfi P.P. (2019). Germline NPM1 mutations lead to altered rRNA 2’-O-methylation and cause dyskeratosis congenita. Nature genetics, 51(10), 1518-1529.
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